Phenolic Compounds and Hydroxyl Radical Scavenging Activities of Aqueous and Methanolic Extracts of Phoenix Dactylifera L Cultivar Dayri Fruits

Document Type : Original Article

Authors

1 Assistant Professor of Pharmacognosy.Deparment of Pharmacognosy, Faculty of Pharmacy, Herbal Medicine & Natural Product Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Pharmacist. Deparment of Pharmacognosy, Faculty of Pharmacy, Herbal Medicine Research Center

3 Pharmacist.Deparment of Pharmacognosy, Faculty of Pharmacy, Herbal Medicine Research Center.

Abstract

Background and Objective: Nutrition and dietary patterns have been shown to have direct impact on health of the population and of selected patient groups. The antioxidant nutrition play an important role in protection against degenerative disease. The fruits of the date palm (Phoenix dactylifera) are consumed throughout the world and are an important part of the diet in Iran. The hydroxyl radical is known to cause significant damage to cellular DNA, proteins and cell wall, leading to cell death and polyphenols showed strong scavenging activity on hydroxyl radicals.
Subjects and Methods: Methanol and aqueous extracts were prepared from fruits of phoenix dactilyfera by maceration method.  deoxyribose method were used to evaluated hydroxyl radical scavenging capacity of herbal extract. Total phenolic content was determined by the Folin–Ciocalteu method. The flavonoids and proanthocyanidins were determined by UV spectrophotometry methods.
Results: The total phenolic, flavonoids and proanthocyanidins content of methanolic and aqueous extracts were 3.77 and 17.42 mg tannic acid equivalents per g dry extract, 0.20 and 0.26 mg rutin equivalents per g dry extract and 0.12, 0.11 mg Cyanidin chloride per g dry extract, respectively. The IC50 value for methanol and aqueous extract in deoxyribose method were determined 0.76 and 0.99 mg/ml.
Conclusion: Methanol and aqueous extracts exhibited hydroxyl radical scavenging activity. However, methanol extracts showed better results, but total phenolic content of aqueous extract was  greater. The two extracts showed almost no differences in flavonoids and proanthocyanidins contents.

Keywords

References

1-Gutteridge JMC, Halliwell B. Antioxidants in nutrition, health, and disease. Oxford: Oxford University Press; 1994.
2-Graf E, Mahoney JR, Bryant RG, Eaton JW. Iron-catalyzed hydroxyl radical formation. Stringent requirement for free iron coordination site. J Biol Chem 1984;259:3620-4.
3-Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr 1993 ;57:715S-24S.
4-Halliwell B. Antioxidants and human disease: a general introduction. Nutr Rev 1997;55:S44-S9.
5-Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157-84.
6-Pedrielli P, Pedulli GF, Skibsted LH. Antioxidant mechanism of flavonoids. Solvent effect on rate constant for chain-breaking reaction of quercetin and epicatechin in autoxidation of methyl linoleate. J Agric Food Chem 2001;49:3034-40.
7-Hashempoori M, Sanei Shariat Panahi M, Daneshvar MH. Identification of date palm cultivars in khozestan province (Shadegan). Iranian J Agric Sci 2003;34:749-55.
8-Boudries H, Kefalas P, Hornero-Mqndez D. Carotenoid composition of Algerian date varieties (Phoenix dactylifera) at different edible maturation stages. Food Chemistry 2007;101:1372-7.
9-Hong YJ, Tomas-Barberan FA, Kader AA, Mitchell AE. The flavonoid glycosides and procyanidin composition of Deglet Noor dates (Phoenix dactylifera). J Agric Food Chem 2006;54:2405-11.
10-Salib JY. Flavonoid glycosides from fruit shells of Phoenix dactylifera L. Asian Journal of Chemistry 2008;20:1593-8.
11-Al-Qarawi AA, Abdel-Rahman H, Ali BH, Mousa HM, El-Mougy SA. The ameliorative effect of dates (Phoenix dactylifera L.) on ethanol-induced gastric ulcer in rats. J Ethnopharmacol 2005;98:313-7.
12-Ghasemi N, Moatar F, Mohagheghzadeh A. Introduction Iranian Herbal pharmacopoeia vol (1). Tehran: Ministry of Health Publication ;2003. p. 1-33.
13-Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165:215-9.
14-Singleton VL, Rossi Jr, JA. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am J Enol Vitic 1965;16:144-58.
15-Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 2000;72:35-42.
16-Hernández I, Alegre L, Van Breusegem F, Munné-Bosch S. How relevant are flavonoids as antioxidants in plants? Trends Plant Sci 2009;14:125-32.
17-Huang D, Ou B, Prior RL. The Chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
18-Stevanato R, Fabris S, Momo F. New enzymatic method for the determination of total phenolic content in tea and wine. J Agric Food Chem 2004;52:6287-93.
19-Al-Shahib W, Marshall RJ. The fruit of the date palm: Its possible use as the best food for the future? Int J Food Sci Nutr 2003;54:247-59.
20-Di Mascio P, Murphy ME, Sies H. Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr 1991;53:194S-200.
21-Paiva SA, Russell RM. Beta-carotene and other carotenoids as antioxidants. J Am Coll Nutr 1999;18:426-33.
22-Vayalil PK. Antioxidant and antimutagenic properties of aqueous extract of date fruit (Phoenix dactylifera L. Arecaceae). J Agric Food Chem 2002;50:610-7.
Jundishapur Scientific Medical Journal
Volume 11, Issue 2 - Serial Number 77
July and August 2017
Pages 185-192

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History

  • Receive Date: 24 October 2009
  • Revise Date: 17 June 2010
  • Accept Date: 17 January 2012

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