A Study of the Effect of Apium graveolens Hydroalcoholic Extract on Formalin-induced Inflammation in Male Rat Hind Paw

Document Type : Original Article


1 Department of Pharmacology, Toxicology, School of Pharmacy, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Deparment of


Background and Objectives: The aim of this study was to compare the inflammatory effect of hydroalcoholic extract of Apium graveolens with aspirin.
Subject and Methods:  The plant was collected from Dezful gardens. Hydroalcoholic extract of Apium graveolens powder was macetrated in ethanol 70% (v/v). Animals were divided into 6 groups (10 for each). Negative group received 5ml/kg normal saline, the positive group received 300 mg/kg aspirin and extract-treated groups received 100, 200, 400, 600 mg/kg of hydroalcoholic extract of Apium graveolens (IP) respectively. Thirty minutes later all groups received 100 µl formalin 2.5% (SC, in hind paw). Hid paw vulome was measured for five hours in intervals of one hour using plethysmometer.
Results: All extract-treated groups showed dose-dependent anti-inflammatory effect. There were no significant differences between the groups that received 400 and 600 mg/kg extract with those received aspirin.
Conclusion: The hydroalcoholic extract of Apium graveolensshowed anti-inflammatory effect that was dose-dependent.


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