The effect of six weeks aerobic training on serum and hepatic ascorbic acid levels and hepatic SVCT2 level in diabetic Wistar rats

Document Type : Original Article


1 Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

2 Department exercise physiology, School of Sport Sciences, Razi University, Kermanshah, Iran.

3 Department of physical education and sport science, Kermanshah branch, Islamic Azad University, Kermanshah, Iran.

4 Department exercise physiology, School of Sport Sciences, Razi University, Kermanshah, Iran


Introduction: Sodium-dependent vitamin C transporter 2 (SVCT2) plays an important role in the transport of ascorbic acid into the cell. The purpose of this study was to investigate the effect of diabetes induction and exercise training on hepatic ascorbic acid and SVCT2 levels in Wistar rats.

Methods: In this experimental study, 20 male Wistar rats were randomly divided into 4 groups: 1) healthy control) diabetes control, 3) diabetes training and 4) sham. After diabetes induction training program consisted of 6 weeks of running on the treadmill, 5 sessions per week and each session of 20 to 40 minutes at a speed of 10-20 m / min was applied. Liver and serum tissues were evaluated to investigate the effect of exercise training on ascorbic acid metabolism.

Results: The results showed that induction of diabetes significantly decreased serum and hepatic ascorbic acid levels in the diabetic and diabetic & exercise groups compared with the control and sham groups (P <0.001). Also, hepatic SVCT2 was significantly increased in the diabetic group compared to the control group (P = 0.041) and significantly increased in the diabetic and diabetic & exercise groups compared to the sham group (P <0.05).
Conclusion: According to the results of this study, induction of diabetes reduces hepatic ascorbic acid levels, this appears to be associated with a decrease in serum ascorbic acid levels, and increased levels of liver SVCT2 are an up regulation mechanism to compensate for lower levels of liver ascorbic acid.


1-Ghalavand A, Motamedi P, Rajabi H, Khaledi N. The effect of six weeks aerobic training on Serum and muscle levels of ascorbic acid and SVCT2 of soleus muscle tissue in Wistar rats. Jundishapur Scientific Medical Journal. 2019;17(5):481-90.
2-Kashiba M, Oka J, Ichikawa R, Kasahara E, Inayama T, Kageyama A, et al. Impaired ascorbic acid metabolism in streptozotocin-induced diabetic rats. Free Radical Biology and Medicine. 2002;33(9):1221-30.
3-Wu X, Iguchi T, Hirano J, Fujita I, Ueda H, Itoh N, et al. Upregulation of sodium-dependent vitamin C transporter 2 expression in adrenals increases norepinephrine production and aggravates hyperlipidemia in mice with streptozotocin-induced diabetes. Biochemical pharmacology. 2007;74(7):1020-8.
4-Amano A, Aigaki T, Maruyama N, Ishigami A. Ascorbic acid depletion enhances expression of the sodium-dependent vitamin C transporters, SVCT1 and SVCT2, and uptake of ascorbic acid in livers of SMP30/GNL knockout mice. Archives of biochemistry and biophysics. 2010;496(1)38-44.
5-Hierro C, Monte MJ, Lozano E, Gonzalez-Sanchez E, Marin JJ, Macias RI. Liver metabolic/oxidative stress induces hepatic and extrahepatic changes in the expression of the vitamin C transporters SVCT1 and SVCT2. European journal of nutrition. 201.53;4(2):401-12.
6-Søgaard D, Lindblad MM, Paidi MD, Hasselholt S, Lykkesfeldt J, Tveden-Nyborg P. In vivo vitamin C deficiency in guinea pigs increases ascorbate transporters in liver but not kidney and brain. Nutrition research. 2014;34(7):639-45.
7-Campos E, Jarrete A, Araujo H, Cayres S, Neto JC, Luciano E. Effect of swimming training on stress-related metabolic parameters of diabetic and non-diabetic rats. Revista Brasileira de Atividade Física & Saúde. 2014;19(2):195.
8-Tian H, Ye X, Hou X, Yang X, Yang J, Wu C. SVCT2, a potential therapeutic target, protects against oxidative stress during ethanol-induced neurotoxicity via JNK/p38 MAPKs, NF-κB and miRNA125a-5p. Free Radical Biology and Medicine. 2016;96:362-73.
9-Sandoval Silva DA. Estudio de la expresión y localización subcelular del transportador de vitamina C (SVCT2) en fenómenos de plasticidad del músculo esquelético. 2012.
10-Savini I, Rossi A, Catani MV, Ceci R, Avigliano L. Redox regulation of vitamin C transporter SVCT2 in C2C12 myotubes. Biochemical and biophysical research communications. 2007;361(2):385-90.
11-Sun L, Shen W, Liu Z, Guan S, Liu J, Ding S. Endurance exercise causes mitochondrial and oxidative stress in rat liver: effects of a combination of mitochondrial targeting nutrients. Life sciences. 2010;86(1-2):39-44.
12-Rodrigues B, Figueroa DM, Mostarda CT, Heeren MV, Irigoyen M-C, De Angelis K. Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovascular diabetology. 2007;6(1):38.
13-Amatyakul S, Chakraphan D, Chotpaibulpan S, Patumraj S. The effect of longā€term supplementation of vitamin C on pulpal blood flow in streptozotocinā€induced diabetic rats. Clinical hemorheology and microcirculation. 2003;29(3, 4):313-9.
14-Carr AC, Bozonet SM, Pullar JM, Simcock JW, Vissers MC. Human skeletal muscle ascorbate is highly responsive to changes in vitamin C intake and plasma concentrations–. The American journal of clinical nutrition. 2013; 97(4):800-7.
15-Contarteze RVL, Manchado FdB, Gobatto CA, Mello MARd. Biomarkers of stress in rats exercised in swimming at intensities equal and superior to the maximal estable lactate phase. Revista Brasileira de Medicina do Esporte. 2007;13(3):169-74.