Effect of Zingiber Officinale extract on Tissue Changes in BALB/c Mice with Systemic Candidiasis

Document Type : Original Article

Authors

1 Infectious and Tropical Diseases Research Center, Health Research Institute and Department of Mycology, school of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahwaz, Iran

2 Department of Mycology, school of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahwaz, Iran

3 Cellular and Molecular research center, Department of Anatomy , Faculty of Medicine, Jundishapur University of Medical Sciences, Ahwaz, Iran

Abstract

Background and Aim: Candida species are the fourth agent of blood infections in patients hospitalized, in which cases of treatment failure and resistance to antifungal drugs have been reported. Ginger is. In this study, the effect of alcoholic extract of ginger, which was used as an immune system stimulant, antimicrobial and antifungal, was surveyed on systemic candidiasis in Balb / c mice.
Materials and Methods: In the experimental study, 49 female mice of Balb / c were randomly divided into seven group, seven mice in each group. After intravenously injection by the standard strain of C. albicans (ATCC 10231), mice were treated by ginger extract with doses of 50, 100 and 200 mg / ml and fluconazole for 7 days. Then the mice were euthanized and evaluated for microbial load of the kidneys, liver, peritoneum liquid and perforated macrophages, and the pathology of kidney and liver tissue was stained with specific staining of GMS.
Results: Ginger extract of 100 mg / ml was able to reduce the microbial load of the kidneys, liver, peritoneum and increase the count of peritoneal macrophages compared to the positive control group. The results of histopathology also confirmed the changes in liver and kidney pathology in the ginger group compared to fluconazole and positive control group in the form of mild, moderate and severe.
Conclusion: The results indicate that ginger extract can act as an effective antifungal agent with good efficacy against invasive candidiasis.

Keywords

References

1-Pfaller M, Diekema D. Epidemiology of invasive candidiasis: a persistent public health problem. Clinical microbiology reviews. 2007;20(1):133-63.
2-Morrell M, Fraser VJ, Kollef MH. Delaying the empiric treatment of Candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrobial agents and chemotherapy. 2005;49(9):3640-5.
3-Looi CY, D'Silva EC, Seow HF, Rosli R, Ng KP, Chong PP. Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients. FEMS microbiology letters. 2005;249(2):283-9.
4-Pemán J, Cantón E, Espinel-Ingroff A. Antifungal drug resistance mechanisms. Expert review of anti-infective therapy. 2009;7(4):453-60.
5-Garvey E, Hoekstra W, Schotzinger R, Sobel J, Lilly E, Fidel P. Efficacy of the clinical agent VT-1161 against fluconazole-sensitive and-resistant Candida albicans in a murine model of vaginal candidiasis. Antimicrobial agents and chemotherapy. 2015;59(9):5567-73.
6-Aghel N, Mahmoudabadi AZ, Darvishi L. Volatile constituents and anti candida activity of the aerial parts essential oil of Dittrichia graveolens (L.) Greuter grown in Iran. African Journal of Pharmacy and Pharmacology. 2011;5(6):772-5.
7-Ficker C, Smith M, Akpagana K, Gbeassor M, Zhang J, Durst T, et al. Bioassay‐guided isolation and identification of antifungal compounds from ginger. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2003;17(8):897-902.
8-Sanwal SK, Rai N, Singh J, Buragohain J. Antioxidant phytochemicals and gingerol content in diploid and tetraploid clones of ginger (Zingiber officinale Roscoe). Scientia Horticulturae. 2010;124(2):280-5.
9-Singh G, Kapoor I, Singh P, de Heluani CS, de Lampasona MP, Catalan CA. Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food and Chemical Toxicology. 2008;46(10):3295-302.
10-Talpur AD, Ikhwanuddin M, Bolong A-MA. Nutritional effects of ginger (Zingiber officinale Roscoe) on immune response of Asian sea bass, Lates calcarifer (Bloch) and disease resistance against Vibrio harveyi. Aquaculture. 2013;400:46-52.
11-Agarwal M, Walia S, Dhingra S, Khambay BPS. Insect growth inhibition, antifeedant and antifungal activity of compounds isolated/derived from Zingiber officinale Roscoe (ginger) rhizomes. Pest Management Science: formerly Pesticide Science. 2001;57(3):289-300.
12- Noshirvani N, Ghanbarzadeh B, Gardrat C, Rezaei MR, Hashemi M, Le Coz C, et al. Cinnamon and ginger essential oils to improve antifungal, physical and mechanical properties of chitosan-carboxymethyl cellulose films. Food Hydrocolloids. 2017;70:36-45.
13-Wang H, Ng TB. An antifungal protein from ginger rhizomes. Biochemical and Biophysical Research Communications. 2005;336(1):100-4.
14- Chrubasik S, Pittler M, Roufogalis B. Zingiberis rhizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomedicine. 2005;12(9):684-701.
15-Doyle TC, Nawotka KA, Kawahara CB, Francis KP, Contag PR. Visualizing fungal infections in living mice using bioluminescent pathogenic Candida albicans strains transformed with the firefly luciferase gene. Microbial pathogenesis. 2006;40(2):82-90.
16-Green CB, Zhao X, Hoyer LL. Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis. Infection and immunity. 2005;73(3):1852-5.
17-Pappas PG, Kauffman CA, Andes DR, Clancy CJ, Marr KA, Ostrosky-Zeichner L, et al. Clinical practice guideline for the management of candidiasis: 2016 update by the Infectious Diseases Society of America. Clinical Infectious Diseases. 2015;62(4):e1-e50.
18-Khodavandi A, Alizadeh F, Harmal NS, Sidik SM, Othman F, Sekawi Z, et al. Comparison between efficacy of allicin and fluconazole against Candida albicans in vitro and in a systemic candidiasis mouse model. FEMS microbiology letters. 2011;315(2):87-93.
19-Nikaein D, Khosravi AR, Moosavi Z, Shokri H, Erfanmanesh A, Ghorbani-Choboghlo H, et al. Effect of honey as an immunomodulator against invasive aspergillosis in BALB/c mice. Journal of Apicultural Research. 2014;53(1):84-90.
20-Atai Z, Atapour M, Mohseni M. Inhibitory effect of ginger extract on Candida albicans. American Journal of Applied Sciences. 2009;6(6):1067-9.
21-Nguefack J, Leth V, Zollo PA, Mathur S. Evaluation of five essential oils from aromatic plants of Cameroon for controlling food spoilage and mycotoxin producing fungi. International Journal of Food Microbiology. 2004;94(3):329-34.
22-Ficker CE, Arnason J, Vindas P, Alvarez L, Akpagana K, Gbeassor M, et al. Inhibition of human pathogenic fungi by ethnobotanically selected plant extracts. Mycoses. 2003;46(1‐2):29-37.
23-Mohammadi R, Moattar F. Antifungal activity of Zingiber officinale Rosc. essential oil against fluconazole resistant vaginal isolates of Candida albicans. Journal of Medicinal Plants. 2007;4(24):22-7.
24-Shabanian S, Khalili S, Lorigooini Z, Malekpour A, Heidari-Soureshjani S. The effect of vaginal cream containing ginger in users of clotrimazole vaginal cream on vaginal candidiasis. Journal of advanced pharmaceutical technology & research. 2017;8(2):80.
25-Ghasemzadeh A, Jaafar HZ, Rahmat AJM. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). 2010;15(6):4324-33.
26- Manohar V, Ingram C, Gray J, Talpur NA, Echard BW, Bagchi D, et al. Antifungal activities of origanum oil against Candida albicans. Molecular and cellular biochemistry. 2001;228(1-2):111-7.
27-Odds FC, Van Nuffel L, Gow NA. Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host. Microbiology. 2000;146(8):1881-9.
28-Yaraee R, Ghazanfari T, Eghtedardoost M, Rajabi M, Naseri M. The effect of MS14 on innate and cellular immune responses in BALB/c mice. Immunopharmacology and immunotoxicology. 2011;33(3):509-14.
Jundishapur Scientific Medical Journal
Volume 18, Issue 2 - Serial Number 119
July and August 2019
Pages 143-151

Files

History

  • Receive Date: 20 May 2019
  • Revise Date: 03 July 2019
  • Accept Date: 13 July 2019

Share

How to cite

Statistics