Evaluation of Co-aggregation and Anti-Adhesive Effects of Selenium Nanoparticles and Selected Probiotic Strains on Clinical and Standard Strains of Index Pathogens

Document Type : Original Article


1 PhD Student of Animal Science.Department of Animal Science, University of Tabriz, Tabriz, Iran.

2 Department of Animal Science, University of Tabriz, Tabriz, Iran.

3 Professor of Animal Science.Department of Animal Science, University of Tabriz, Tabriz, Iran.

4 Assistant Professor of Animal Science.Department of Animal Science, University of Tabriz, Tabriz, Iran.


Background and Objectives: One of the most important factors in pathogenic disease is the attachment of pathogenic bacteria to the host cell surface. The present study was conducted to introduce an alternative bio-drug that prevents the attachment of microorganisms and the onset of disease.
Subjects and Methods: This study was carried out during the three experimental periods. Clinical and standard strains of E. coli and C. albicans yeast were collected from the Khalat-Pushan Research Station of Tabriz University and Iran Research Organization respectively. Co-aggregation activity of treatments with both E. coli and C. albicans were investigated by Collado method and expressed as percentage. Anti-adhesive activity was also determined by microtitre plate method and calculated by reduction in attachments percentage.
Results: The highest co-aggregation effect with 74.68 % was related to “probiotic+ nano-selenium” treatment with standard strain of C. albicans and “nano-selenium”. While treatment with 39.32% had the lowest co-aggregation activity with clinical strain of E. coli (P<0.05). In determination of anti-adhesive activity of experimental treatments, except for the “probiotic” treatment on clinical strain of C. albicans, there was no significant difference among treatments for both species of E. coli and C. albicans. In both strains of E .coli and C. albicans, the standard strains showed significantly higher sensitivity .
Conclusion: All the experimental treatments had a good co-aggregation and anti-adhesive ability with pathogenic species. “Probiotic + nano-selenium” treatment had relatively high ability during three experimental protocols Therefore, it can be used as an appropriate antibiotic alternative for the prevention or control of disease.


1-Cheraghi Saray S, Hosseinkhani A, Janmohammadi H, Zare P, Daghighkia H. Thermal and probiotic treatment effects on restaurant waste for incorporation into poultry diet. Int J Recycl Org Waste Agricult. 2014; 3: 1-7.
2-Chung YC, Su YP, Chen CC, Jia G, Wang Hl, Wu JG, Lin JG. Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacol Sin. 2004; 25: 932-6.
3-Soleimani NA, Kermanshahi RK, Yakhchali B, Sattari TN. Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis. Afr J Microbiol Res. 2010; 4: 2169-73.
4-Reid G, McGroarty JA, Angotti R, Cook RL. Lactobacillus inhibitor production against Escherichia coli and co aggregation ability with uropathogens. Can J Microbiol. 1988; 34: 344-51.
5-Goh YJ, Klaenhammer TR. Functional roles of aggregation-promoting-like factor in stress tolerance and adherence of Lactobacillus acidophilus NCFM. Appl Environ Microbiol. 2010; 76: 5005-12.
6-Huh AJ, Kwon YJ. "Nanoantibiotics": a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J Controlled Release. 2011; 2: 128–45.
7-Kamada H, Nonaka I, Ueda Y, Murai M. Selenium addition to colostrum increase immunoglobulin G absorption by newborn calves. J Dairy Sci. 2007; 90: 5665-70.
8-Zawrah MF, Abd El-moez SI. Antimicrobial activities of gold nanoparticles against major foodborne pathogens. Life Sci J. 2011; 8: 37-44.
9-Shokohi T, Hashemi Soteh MB, Saltanat Pouri Z, Hedayati MT, Mayahi S. Identification of Candida species using PCR-RFLP in cancer patients in Iran. Indian J Med microbial. 2010; 28: 147-51.
10-Masuoka J. Surface glycans of Candida albicans and other pathogenic fungi: physiological roles, clinical uses, and experimental challenges. Clin Micro Rev. 2004; 17: 281-310.
11-Otang WM, Grierson DS, Ndip RN. Antifungal activity of Arctotis arctotoides (L.f.) O. Hoffm and Gasteria bicolor Haw against opportunistic fungi associated with human immunodeficiency virus/acquired immunodeficiency syndrome. Pharmacogn Mag. 2012; 8: 135-40.
12-Stabnikova O, Ivanov V, Larionova I, Lewis J. Ukrainian dietary bakery product with selenium-enriched yeast. LWT-Food Sci Technol. 2008; 41: 890-5.
13-Hongfei Y, Gongjian F, Zhenxin G. Optimization of culture parameters of selenium-enriched yeast (Saccharomyces cerevisiae) by response surface methodology (RSM). LWT-Food Sci Technol. 2010; 43: 666-9.
14-Collado MC, Meriluoto J, Salminen S. Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Technol. 2008; 226: 1065-73.
15-Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn S, Versalovic J. Human- derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. 2008; 14: 166-71.
16-Santos CMA, Pires MCV, Leao TL, Hernandez ZP, Rodriguez ML, Martins AKS, Miranda LS, Martins FS, Nicoli JR. Selection of Lactobacillus strains as potential probiotics for vaginitis treatment. Microbiol. 2016; 162: 1195-207.
17-Jorgensena MR, Kragelunda C, Jensenb PO, Kellera MK, Twetman S. Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro. J Oral Microbiol. 2017; 9: 1274582.
18-Do-Carmo MS, Noronha FMF, Arruda MO, Costa EPS, Bomfim MRQ, Monteiro AS, Ferro TAF, Fernandes ES, Giron JA, Monteiro-Neto V. Lactobacillus fermentum ATCC 23271 displays in vitro inhibitory activities against Candida spp. Front Microbiol. 2016; 7:1722.
19-Kos B, Susković J, Vukovic S, Simpraga M, Frece J, Matosic S. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol. 2003; 94: 981-7.
20-Angmo K, Savitri AK, Bhalla TC. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT Food Sci Technol. 2016; 66: 428-35.
21-Zhang Y, Zhang L, Du M, Yi H, Guo C, Tuo Y, Han X, Li J, Zhang L, Yang L. Antimicrobial activity against Shigella sonnei and probiotic properties of wild lactobacilli from fermented food. Microbiol Res. 2011; 167: 27– 31.
22-Nikaido H. Prevention of drug access to bacterial targets: Permeability barriers and active efflux. Sci. 1994; 264: 382–8.
23-Danese PN, Pratt LA, Kolter R. Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture. J Bacteriol. 2000; 182: 3593–6.
24-Ingrassia I, Leplingard A, Darfeuille-Michaud A. Lactobacillus casei DN-114 001 inhibits the ability of adherent-invasive Escherichia coli isolated from Crohn's disease patients to adhere to and to invade intestinal epithelial cells. Appl Environ Microbiol. 2005; 71: 2880-7.
25-Abedi D, Feizizadeh S, Akbari V, Jafarian-Dehkordi A. In vitro anti-bacterial and anti-adherence effects of Lactobacillus delbrueckii subsp bulgaricus on Escherichia coli. Res Pharm Sci. 2013; 8: 260-8.
26-Kaur R, Kaudal T, Sharma A. Probiotic mediated synthesis of selenium particles: characterization and biofilm scavenging analysis. Res J Life Sci Bioinf Pharm Chem Sci. 2018; 4: 291-304.
27-Guisbiers G, Lara HH, Mendoza-Cruz R, Naranjo G, Vincent BA, Peralta XG, Nash KL. Inhibition of Candida albicans biofilm by pure selenium nanoparticles synthesized by pulsed laser ablation in liquids. Nanomed Nanotechnol. 2017; 13: 1095-103.
28-Dwivedi P, Thompson A, Xie Z, Kashleva H, Ganguly S, Mitchell AP, Dongari-Bagtzoglou A. Role of Bcr1-activated genes Hwp1 and Hyr1 in Candida albicans oral mucosal biofilms and neutrophil evasion. PLoS One. 2011; 6: e16218.