Effect of High Interval Intensity Training and Aerobic Exercise on the Content of the SERCA2a and Phospholamban Proteins in Slow-Twitch and Fast Twitch Muscles of Wistar Rat

Document Type : Original Article

Authors

1 PHD Student of Exercise Physiology.Department of Exercise Physiology, Faculty of Sport Science, Esfahan University, Esfahan, Iran.

2 Professor of Exercise Physiology.Department of Exercise Physiology, Faculty of Sport Science, Esfahan University, Esfahan, Iran.

Abstract

Background and Objective: The control of cytosolic calcium is precisely due to its major role in the muscle contraction. So, we investigated the changes in FULL NAME FIRST (SERCA2a), the most important calcium transmitter, and its inhibitory, (Phospholamban Proteins (PLN), with exercise.
Subjects and Methods: Eighteen adult male Wistar rats were placed in three groups, aerobic exercise training (AET), high intensity interval training (HIIT), and control group (CO) .The AET and HIIT groups ran on treadmill at speeds of 12-15 (m/min), and 25 to 30 (m/min), respectively, 5 days a week for 8 weeks. At the end, the extensor digitorum longus (EDL) and soleus (sol) muscles were extracted and the concentration of SERCA2a and PLN proteins were measured using ELISA kits. One way ANOVA, independent t-test, and two-way analyze of variance at the significance level (α = 0.05) were used.
Results: In terms of SERCA2a, there was a significant difference between the EDL and SOL muscles in the control group (P=0.001) and the AET group (P=0.030). Also, there was a significant difference in PLN between EDL and SOL muscles only in the AET group (P=0.007). Also, there was a significant difference between the control groups, AET and HIIT in terms of SERCA2a (P=0.046) and PLN (P=0.006). In addition, an interactive effect was observed between exercise intensity and muscle fiber type on the SERCA2a (P =0.042) and also PLN (P = 0.008).
Conclusion: To achieve optimal muscle function, especially factors affecting calcium transmission, it is necessary to consider simultaneously the type of muscle fiber and the intensity of exercise appropriate to it.

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 17, Issue 3 - Serial Number 114
September and October 2018
Pages 315-328

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History

  • Receive Date: 14 May 2018
  • Revise Date: 25 June 2018
  • Accept Date: 03 July 2018

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