Identification and Quantification of Gliclazide in Human Plasma by HPLC-PDA

Document Type : Original Article

Authors

1 Farmacy Student, Faculty of Farmacy.Faculty of Pharmacy, Ph.D. Student of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 ahvaz jundishapour university medical science, pharmacy factuly

3 Associate Professor of Genetics.Department of Medical Genetic, Factuly of Medicine, Ahvaz Jundishapur Universy Medical Sciences, Ahvaz, Iran.

4 Associate Professor of Internal Medicine, Diabetic Research Center.Diabetes Research Center,Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Background and Objective: In this study, a high-performance liquid chromatography method was developed to determine the concentration of gliclazide in plasma. The Photodiode array (PDA) detector allows for the determination of purity.
Subjects and Methods: The mobile phase used in this study was phosphate buffer with pH = 3.5 and acetonitrile (HPLC grade) at a ratio of 1: 1 was run isocraticaly through a C18 analytical column. The UV detection was done at 253 nm. Analytical run time was less than 10 min.
Results: In the optimum conditions, the calibration curve in the area of ​50 to 400 ng/ml, linear with the equation y = 28.029x + 212.74 and 0.998 = R2. Gliclazide was detected within 3.6 minutes by photodiode detector. The limit of detection (LOD) and the limit of quantitative (LOQ) in this method are calculated as 23 ng/ml and 71 ng/ml, respectively.
Conclusion: The evaluation of the accuracy and precision of method showed that this method has the ability to determine the amount of gliclazide in plasma, and the method is reliable and fast.

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 17, Issue 2 - Serial Number 113
July and August 2018
Pages 195-201

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History

  • Receive Date: 18 February 2018
  • Revise Date: 28 May 2018
  • Accept Date: 28 May 2018

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