Identification and Quantification of Gliclazide in Human Plasma by HPLC-PDA

Document Type : Original Article


1 Farmacy Student, Faculty of Farmacy.Faculty of Pharmacy, Ph.D. Student of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 ahvaz jundishapour university medical science, pharmacy factuly

3 Associate Professor of Genetics.Department of Medical Genetic, Factuly of Medicine, Ahvaz Jundishapur Universy Medical Sciences, Ahvaz, Iran.

4 Associate Professor of Internal Medicine, Diabetic Research Center.Diabetes Research Center,Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran


Background and Objective: In this study, a high-performance liquid chromatography method was developed to determine the concentration of gliclazide in plasma. The Photodiode array (PDA) detector allows for the determination of purity.
Subjects and Methods: The mobile phase used in this study was phosphate buffer with pH = 3.5 and acetonitrile (HPLC grade) at a ratio of 1: 1 was run isocraticaly through a C18 analytical column. The UV detection was done at 253 nm. Analytical run time was less than 10 min.
Results: In the optimum conditions, the calibration curve in the area of ​50 to 400 ng/ml, linear with the equation y = 28.029x + 212.74 and 0.998 = R2. Gliclazide was detected within 3.6 minutes by photodiode detector. The limit of detection (LOD) and the limit of quantitative (LOQ) in this method are calculated as 23 ng/ml and 71 ng/ml, respectively.
Conclusion: The evaluation of the accuracy and precision of method showed that this method has the ability to determine the amount of gliclazide in plasma, and the method is reliable and fast.


1-Bressler R, Johnson DG. Pharmacological Regulation of Blood Glucose Levels in Non—Insulin-Department Diabetes Mellitus. Archives of internal medicine. 1997;157(8):836-48.
2-Palmer KJ, Brogden RN. Gliclazide. Drugs. 1993;46(1):92-125.
3-Matsuda R, Anguizola J, Joseph K, Hage DS. High-performance affinity chromatography and the analysis of drug interactions with modified proteins: binding of gliclazide with glycated human serum albumin. Analytical and bioanalytical chemistry. 2011;401(9):2811.
4-Sarkar A, Tiwari A, Bhasin PS, Mitra M. Pharmacological and Pharmaceutical Profile of Gliclazide: A Review. 2011.
5-Musshoff F, Madea B. Review of biologic matrices (urine, blood, hair) as indicators of recent or ongoing cannabis use. Therapeutic drug monitoring. 2006;28(2):155-63.
6-Agrawal Y, Gogoi P, Manna K, Bhatt H, Jain V. A supercritical fluid chromatography/tandem mass spectrometry method for the simultaneous quantification of metformin and gliclazide in human plasma. Indian journal of pharmaceutical sciences. 2010;72(1):50.
7-Główka FK, Łada MK, Grund G, Wachowiak J. Determination of treosulfan in plasma and urine by HPLC with refractometric detection; pharmacokinetic studies in children undergoing myeloablative treatment prior to haematopoietic stem cell transplantation. Journal of Chromatography B. 2007;850(1-2):569-74.
8-Nekrassova O, Lawrence NS, Compton RG. Analytical determination of homocysteine: a review. Talanta. 2003;60(6):1085-95.
9-Ranetti M-C, Ionescu M, Hinescu L, Ionica E, Anuta V, Ranetti AE, et al. Validation of a HPLC method for the simultaneous analysis of metformin and gliclazide in human plasma. Farmacia. 2009;57(6):728-35.
10-Venkatesh P, Harisudhan T, Choudhury H, Mullangi R, Srinivas NR. Simultaneous estimation of six anti‐diabetic drugs—glibenclamide, gliclazide, glipizide, pioglitazone, repaglinide and rosiglitazone: development of a novel HPLC method for use in the analysis of pharmaceutical formulations and its application to human plasma assay. Biomedical Chromatography. 2006;20(10):1043-8.
11-Jamadar SA, Mulye SP, Karekar PS, Pore YV, Burade KB. Development and validation of UV spectrophotometric method for the determination of Gliclazide in tablet dosage form. Der Pharma Chemica. 2011;3(4):338-43.
12-Bhaskar R, Sagar M, Saini V, Bhat K. UV-spectrophotometric-assisted chemometric methods for the simultaneous determination of metformin hydrochloride and gliclazide in pharmaceutical formulations. Pharmaceut Anal Acta. 2012;3(4):1-5.