Impact of Consumption of a Single Course of Probiotic Supplementation on Malondialdehyde, Total Antioxidant Capacity and Lactic Acid in Futsalist Women

Document Type : Original Article


1 abadan islamic azad university

2 Assistant Professor of Physical Education.Department of Physical Education, Islamic Azad University, Abadan Unit, Abadan, Iran.

3 Ph.D Student of Motor Behavior.Department of Motor Control and Learning, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran.

4 Msc Student Healh Care Mangment.Faculty of Health, Ahvaz Jundishapur University Medical Sciences, Iran.


Background and Objective: Nowadays, the use of probiotic supplements to prevent the production of free radicals has become more common. The aim of the present study was to assess the impact of taking a single course of probiotic supplementation on MDA, total antioxidant capacity and lactic acid in futsalist women.
Subjects and Methods: In a semi experimental study, women futsalists of Ahwaz city were selected through available sampling method and divided into two experimental and control groups of 15. Based on the findings of this study, probiotic supplements can be used to reduce the oxidative damage of intense exercise. Subjects with a range of 23 ± 0/60 years, height 167.4 ± 16, weight 87.50 ± 7.50 kg, and body mass index of 27.1 ± 4.9, were exercises for eight weeks, three sessions per week and each training session was 75 minutes. Long Supplements and placebo were given to the subjects in the placebo capsule. Blood samples were taken from the subjects in the post-test phase. For statistical analysis, ANOVA and independent t-test were used (P ≤ 0.05).
Results: The results showed that there was a significant decrease in MDA (P ≤ 0.05) and significant increase in antioxidant capacity (P ≤ 0.05) among the probiotic supplementation group compared to control group. However, there was no significant difference in reduction lactic acid between both groups (P ≤ 0.05).
Conclusion: According to the findings of this study, the use of probiotic supplementation can improve the antioxidant capacity in athletes.


1-Ghadamli L, Salarkia N, Zayeri F , Sabaghian Rad L.  Effects of probiotic yogurt on performance, respiratory infections, and digestive disorders of endurance young adult women-swimmers.Iranian Journal of Nutrition Sciences & Food Technology2010:9(18).
2-Cox AJ, Gleeson M, Pyne DB, Callister R, Hopkins WG, Fricker PA. Clinical and laboratory evaluation of upper respiratory symptoms in elite athletes. Clin J Sport Med 2008; 18(5):438-45.
3-Mackinnon LT. Chronic exercise training effects on immune function. Med Sci Sports Exerc 2000 Jul; 32(7 Suppl):76-362
4-Pyne DB, Gleeson M. Effects of intensive exercise training on immunity in athletes. J Sports Med 1998 Jul; 19 Suppl3:S83-91.
5-Gleeson M. Mucosal immune responses and risk of respiratory illness in elite athletes. Exerc Immunol Rev 2000;6(42).
6-Szajewska H, Kotowska M, Mrukowicz JZ, Armańska M, Mikołajczyk W. Efficacy of Lactobacillus GG in prevention of nosocomial diarrhea in infants. J Pediatr 2001 Mar; 138(3):361-5.
7-Rodas G, Ventura JL, Cadefau JA, Cussó R, Parra J. A short training programme for the rapid improvement of both aerobic and anaerobic metabolism. Eur App Physiol 2000;82(5-6):480-6.
8-Jahani Ghaeh Ghashlagh  Gh  , Abcar A,  Haydari A. he Effect of High-Intensity Intermittent Training (HIIT) and Consumption of Probiotic Supplement on Immune Cells, C – reactive Protein, and IgA in Young Football Player. Qom University of Medical Sciences Journal2016;8(36-46).
9-Hatakka KSavilahti EPönkä AMeurman JHPoussa TNäse LSaxelin MKorpela R. Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind, randomised trial. BMJ. 2001 Jun 2;322(7298):1327.
10-Ghadamali L, Salarkia N, Zayeri F, Sabaghian Rad L. Effects of probiotic yogurt on performance, respiratory infections, and digestive disorders of endurance young adult women-swimmers. Iranian J Nutri Sci Food Technol 2010;5(2):9-18.
11-Sadeqhi S, Rahimi R. GH and IGF-1 hormone response to resistance two different high volume of rest between sets. Olympic 2009;1:57-68.
12-Dabidiroshan V, Mahmoudi, AA, Joulazadeh T. Comparison of the Effects of aerobicinterval training sessions 3 and 5 of on HS-CRP Wistar rats. J Olympic 2008;17:105-19.
13-Gharakhanlou R, Afzalpour ME, Gaeini, AA, Rahnama, N. Effects of aerobic exercises on the serum Paraoxonase 1/Arylesterase activity and lipid profile in non active healthy men.J Sports Sci Engin 2007;2:105-12.
14-Ugras AF. Effect of high intensity interval training on elite athletes’ antioxidant status. SciSport 2013;28:253-9.
15-Lamprecht M1, Bogner S, Schippinger G, Steinbauer K, Fankhauser F, Hallstroem S, Schuetz B, Greilberger JF. Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial. J Int Soc Sports Nutr. 2012 Sep 20;9(1):45.
16-Koksal H, Kurban S. Total oxidant status, totals antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics ,2014.65(3): 285-290
17-Homayouni-Rad A, Mohtadi-Nia J, Niafar M, Ejtahed H. Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition journal 2012: 28 539–543.
18-Shahsavari Gh, Tootabi A, Raoufi A. The assessment of serum levels of malondialdehyde and total antioxidant capacity after the use of atorvastatin in patients with coronary artery stenosis. Quarterly Yafteh Journal2014: 4(18-26).
19-Jafari A, Zekri R , Dehghan G ,  Malekirad AA. Effect of short-term garlic extract supplementation on oxidative stress and inflammatory indices in non-athlete men after an aerobic exercise. Journal of Cell &Tissue2011:2(1)   25-33.
20-Alessio HM, Hagerman AE, Fulkerson BK. "Generation of reactive oxygen species after exhaustive aerobic and isometric exercise". Med Sci Sport Exercise.2014. 33:PP: 1575-1581.
21-Martarelli D, Scuri S, Cocchioni M , Silvi S, Cecchini C,  Pompei P. Effect of a Probiotic Intake on Oxidant and Antioxidant Parameters in Plasma of Athletes During Intense Exercise Training. Journal of Current Microbiology2011 62(6) 1689–1696.
22-Vaile J, Halson S, Gill N. Dawson. Effect of cold water immersion on repeat cycling performance and thermoregulation in the heat. Journal of Sport Sciences 2008; 26: 431- 440.
23-Gaieni  A, Darianoush F. Sports Physiology, Combining Science and Opinion.  Hatmi Publisher.Tehran,Iran.
24-Fabian E, Majchrzak D, Dieminger B, Meyer E, Elmadfa I (2008) Influence of probiotic and conventional yoghurt on the status of vitamins B1, B2 and B6 in young healthy women. Ann Nutr Metab 52:29–36.
25-Aebi H. Catalase in vitro. Methods in Enzymology 1984; 105 121–6.