The Effect of Intraperitoneal Injection of Ferulae Angulata Hydroalcoholic Extract on Pentylenetetrazole-Induced Epilepsy in Male Mice

Document Type : Original Article


1 Student of the Department of Biology.Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Izeh Unit, Izeh, Iran.

2 Department of Biology, Faculty of Sciences, Izeh Branch, Islamic Azad University, Izeh, Iran


Background and Objectives: Seizure is one of the most common symptoms of epilepsy caused by abnormal electrical activity of neurons; Because of the side effects of chemical drugs the use of herbal medicines can be a good alternative to seizure control. In this study the effectiveness of Ferulago angulata (Schlecht, Boisss) hydroalcoholic extract was investigated in pentylenetetrazole (PTZ)-induced seizure in male mice.
Subjects and Methods:In this experimental study, 48 male mice weighing 25-30g were divided into 6 groups': saline; positive control group (received diazepam as standard an anticonvulsant drug at 1 mg/Kg) and 4 experimental groups (100, 200, 400, 800mg/Kg of extract) respectively. All the animal groups received intraperitoneally pentylenetrazole (85 mg/Kg), 30 minutes after appropriate treatment protocol.
Results: Injection of Ferulago angulata hydroalcoholic extract delayed the onset of seizure at 400 dose (P<0.001), and reduced the duration of clonic seizure, which had the highest effect in doses of 200 and 400 mg/kg PFerulago angulata caused a decrease in the duration of tonic-clonic and decreased the total duration of seizure in animals. IN COMAPRISON WITH DIAZEPAM, WHAT WHERE THE RESULTS? PROVIDE DATA ON BOTH..
Conclusion: In general, intraperitoneal injection of Ferulago hydroalcoholic extract causes delay in attacks in various phases of seizure and it seems the future studies will be necessary to separate its ingredients and understand its mechanism of action.


1-Rahmati B, Khalili M, Roghani M, Ahghari P. Anti-epileptogenic and antioxidant effect of Lavandula officinalis aerial part extract against pentylenetetrazol-induced kindling in male mice. Journal of ethnopharmacology. 2013;148(1):152-7.
2-Amoateng P, Woode E, Kombian SB. Anticonvulsant and related neuropharmacological effects of the whole plant extract of Synedrella nodiflora (L.) Gaertn (Asteraceae). Journal of pharmacy & bioallied sciences. 2012;4(2):140-8.
3-Majores M, Schoch S, Lie A, Becker AJ. Molecular neuropathology of temporal lobe epilepsy: complementary approaches in animal models and human disease tissue. Epilepsia. 2007;48 Suppl 2:4-12.
4-Kiasalari Z, Khalili M, Roghani M, Heidari H, Azizi Y. Antiepileptic and Antioxidant Effect of Hydroalcoholic Extract of Ferula Assa Foetida Gum on Pentylentetrazole- induced Kindling in Male Mice. Basic and clinical neuroscience. 2013;4(4):299-306.
5-Mirzapour S, Rafieirad M, Rouhi L. Hydroalcoholic Extract of Ferulago angulata Improves Memory and Pain in Brain Hypoperfusion Ischemia in Rats. Jundishapur journal of natural pharmaceutical products. 2015;10(1):e17451.
6-Mahmoud Rafieian-kopaei, Najmeh Shahinfard, Hojjat Rouhi-Boroujeni, Mojgan Gharipour, Darvishzadeh-Boroujeni P. Effects of Ferulago angulata Extract on Serum Lipids and Lipid Peroxidation. Evidence-Based Complementary and Alternative Medicine. 2014:1-4.
7-Umukoro S, Omogbiya IA, Eduviere AT. Evaluation of the effect of jobelyn((R)) on chemoconvulsants-induced seizure in mice. Basic and clinical neuroscience. 2013;4(2):125-9.
8-Sierra-Paredes G, Sierra-Marcuno G. Extrasynaptic GABA and glutamate receptors in epilepsy. CNS & neurological disorders drug targets. 2007;6(4):288-300.
9-Pellow S, File SE. Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacology, biochemistry, and behavior. 1986;24(3):525-9.
10-Quintans Júnior LJ, Almeida JRGS, Lima JT, Nunes XP, Siqueira JS, Oliveira LEGd, et al. Plants with anticonvulsant properties: a review. Revista Brasileira de Farmacognosia. 2008;18:798-819.
11-Limberger RP, Aleixo AM, Fett-Neto AG, Henriques AT. Bioconversion of (+)- and (-)-alpha-pinene to (+)- and (-)-verbenone by plant cell cultures of Psychotria brachyceras and Rauvolfia sellowii2007.
12-Abdolhossein Rustaiyan SS, Kambiz Larijani, Morteza Khossravi, and Shiva Masoudi. Composition of the Essential Oil of Ferulago angulata (Schlecht.) Boiss. from Iran. Journal of Essential Oil Research. 2002;14(6):447-8.
13-Aoshima H, Hamamoto K. Potentiation of GABAA receptors expressed in Xenopus oocytes by perfume and phytoncid. Bioscience, biotechnology, and biochemistry. 1999;63(4):743-8.
14-Shahbazi Y, Shavisi N, Karami N, SH. K. Chemical Composition and in Vitro Antibacterial Activity of Ferulago angulata (Schlecht.) Boiss Essential Oil. Pharmaceutical Sciences. 2015;21:6-11.
15-Elisabetsky E, Brum LF, Souza DO. Anticonvulsant properties of linalool in glutamate-related seizure models. Phytomedicine : international journal of phytotherapy and phytopharmacology. 1999;6(2):107-13.
16-Buchbauer G, Jirovetz L, Jager W, Dietrich H, Plank C. Aromatherapy: evidence for sedative effects of the essential oil of lavender after inhalation. Zeitschrift fur Naturforschung C, Journal of biosciences. 1991;46(11-12):1067-72.
17-S, Elsharif SA, Janzen D, Buettner A, Villmann C. Metabolic Products of Linalool and Modulation of GABAA Receptors. Frontiers in chemistry. 2017;5:46.
18-Re L, Barocci S, Sonnino S, Mencarelli A, Vivani C, Paolucci G, et al. Linalool modifies the nicotinic receptor-ion channel kinetics at the mouse neuromuscular junction. Pharmacological research. 2000;42(2):177-82.