Evaluation of SLC30A8 Gene Polymorphism (rs13266634) Distribution in Patients with Diabetes Type 2 in West Mazandaran – Iran

Document Type : Original Article

Authors

1 Graduate Student of Genetics , Sana non-profit institution, Sari, Iran.

2 Associate Professor of Toxicology and Pharmacology.Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

3 Assistant Professor of Toxicology and Pharmacology.Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

4 Assistant Professor of Plant Physiology.Department of Plant Physiology, University of Sana, Mazandaran, Sari, Iran.

5 Assistant Professor of Plant Physiology.Department of Graduate, Sana Non-Profit Institution, Sari, Iran.

6 Graduate Student of Genetics.Department of Graduate, Sana Non-Profit Institution, Sari, Iran.

Abstract

Background and Objective: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic disorders characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The SLC30A8 gene is considered as one of the most important candidate genes of  type 2 diabetes. The objective of this study was to evaluate the distributuion of this gene among our patients in Mazandarn, Iran.
Subjects and Mehtods: In this case-control study, 135 patients and 76 healthy subjects were recruited. Five ml of peripheral blood of diabetics and healthy subjects (controls) was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and genotype and allele frequencies of the rs13266634 polymorphism were investigated using PCR-RFLP method.
Results: Genotype and allele frequencies of the rs13266634 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0003 and P = 0.0664 , respectively). The frequency of C allele was 89% in type 2 diabetes group and 67% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 10.0, 95% CI  1.9124 – 591.3058 , 2.924 – 924.239).
Conclusion: Our results confirm the association between the rs13266634 polymorphism of the SLC30A8 gene and the increased risk of type 2 diabetes among the West Mazandaran population.

Keywords

References

 
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Jundishapur Scientific Medical Journal
Volume 17, Issue 1 - Serial Number 112
March and April 2018
Pages 63-70

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History

  • Receive Date: 20 November 2017
  • Revise Date: 17 January 2018
  • Accept Date: 16 January 2018

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