Comparison of Antibiotic Susceptibility Testing of Escherichia Coli and Klebsiella Pneumonia Isolated from Urinary Tract infections against Five Antibiotics by Disc Diffusion and Microdilution Methods

Document Type : Original Article


1 of Microbiology. Department of Microbiology (, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran.

2 Assistant Professor, Department of Nursing, College of Nursing, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran.

3 Assistant Professor of Nursing. Department of Nursing, College of Nursing, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran.


Background and Objective: Different microorganisms can cause urinary tract infections, but Enterobacteriaceae family, like Escherichia coli and Klebsiella, are the most causative agents of urinary tract infections. So, in this study drug susceptibility testing of Klebsiella pneumonia and Escherichia coli (E.coli) was done to five common antibiotics.
Subjects and Method:This cross – sectional study was done on 100 samples (50 samples of Klebsiella, 50 samples of E.coli) isolated from patients with urinary infections (81 female, 19 male) with mean range 43.3±18.15 y on which biochemical separating tests and Gram staining was done for accurate identification of these  strains. Antibiotic susceptibility test was done by disk diffusion (Kirby Bauer) and microdilution method in accordance with CLSI M7 guideline. Obtained data were analyzed by SPSS software 17 and values of PResults: From 50 strains of E.coli, 45 strains to imipenem, 38 strains to ceftriaxone and ciprofloxacin, 18 strains to co- amoxiclauv and 19 strains to co-trimoxazole were susceptible but from 50 strains of Klebsiella, 45 strains were susceptible to imipenem, 35 strains to ceftriaxone, 37 strains to ciprofloxacin, 20 strains to co- amoxiclauv and 21 strains to co-trimoxazole.
Conclusion:The type of method used in antibiotic susceptibility testing has no effect on results but spontaneous and irregular use of antibiotics should be controlled.


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