Effect of Essential Oil of Leaf and Aerial Part of Rosmarinus officinalis on Passive Avoidance Memory in Aged and Young Mice

Document Type : Original Article


1 Department of Pharmacology and Toxicologi , College of Pharmacy and Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Pharm D.

3 Department of Pharmacology and Toxicologi, College of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Department of of Pharmacogenosy, College of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran


Background and Objective: In this study, the effect of essential oil of aerial part of Rosmarinus officinalis, which is rich of antioxidants, was investigated on memory of young and aged mice with passive avoidance apparatus.
Materials and Methods: Five groups of each young and aged mice (n = 8) were selected for this study. All doses of essential oils of leafand aerial part of Rosmarinus officinalis (200, 400, 600, 800 mg/kg) were injected intraperitoneally once a day for 7 days to four groups of age and young mice and fifth group (control) received 10 ml/kg distilled water once daily for 7 days.
Results: Mean of step-down latency on day 4 in comparison with day 2 became significantly longer (P<0.05) in all young and aged groups of mice. On the other hand, the mean of step-down latency in all groups received different doses of essential oil to compare with control group showed significant (P< 0.05) improvement in memory test in day 4. Also in all aged group received different doses of essential oil step-down latency were significantly (P<0.05) longer than young groups.
Conclusion:The result showed that, the essential oil of Rosmarinus officinalis improves memory in all young and aged animal groups, but it was more effective on aged mice.


1-Karami Nouri R. Pharmacology of learning and memory. 1st ed. Tehran: Samt; 2003: P. 1081-2.
2-Guyton A, Hall J. Medical Physiology. Translated by Shadan F. 2nd ed. Tehran: Chehr; 2000 Vol. 2:  P. 226-32.
3-Zargari A. Medicinal plant. 4th ed. Tehran: Tehran Univercity Prss; 1969: P.71-76
4-Dermarderosian A. The Review of NaturalProducts. 1 st ed. Missouri: Wolters Kluwer Co; 2001, p. 512-13.
5-De Feo V, Senatore F. Medicinal plants and phytotherapy in the Amalfitan coast, Salerno province, Campania, Southern Italy. J Ethnopharmacol 1993 May; 39(1) : 39-51.
6-Martinez-Lirola MJ, Gonzalez-Tejero MR, Molero-Mesa J. Ethanobotanical resources in the province of Almeria, Spain: Campos De Nijar. Econ  Bot 1996 Jan-March; 50(1): 40-56.
7-Chandler F. Memory stimulat: herbal medicine. Can  Pharm  J 1995; 28: 40-53.
8-Ozcan M. Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. J Med Food 2003; 6: 267-70.
9-Kosaka K, Yokoi T. Carnosic acid, acomponent of rosemary (Rosmarinus officinalisL.), promotes synthesis of nerve growth factorin T98G human glioblastoma cells. Biol Pharm Bull 2003Nov; 26(11): 1620-2.
10-Hossein zadeh H, Nourbakhsh M. Effect of Rosmarinus officinalis L. aerial parts extract on morphine with drawal syndrome in mice. Phytother. Res. 2003; 17: 938-41.
11-Sotelo-Felix JI, Martinez-Fong D, Muriel P, Santillan RL, Castillo D, Yahuaca P. Evaluation of the effectiveness of Rosmarinus officinalis(Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat. J Ethnopharmacol 2002; 81: 145-54.
12-Sudha S, Lakshmana MK, Paradhan N. Chronic phenytoin induced impairment of learning and memory with associated changes in brain acetylcholine esterase activity and monoamine levels. Pharmacol  Biochem Behav 1995; 52: 119-24.
13-Chung YK, Heo HJ, Kim EK, Kim HK, Huh TL, Lim Y, Kim SK. Inhibitory effect ofursolic acid purified from Organium majoranalon the acetylcholinesterase. Mol Cells 2001 Apr; 11: 137-143.
14-Anonymous. Quick Access ProfessionalGuide to Conditions, Herbs and Supplements. 1 st ed. Newton: Integrative medicinecommunications 2000; 78.
15-Buccafusco JJ, Terry AV. Multiple centralnervous system targets for eliciting beneficialeffects on memory and cognition. J Pharmacol  Exp Ther 2000 Nov; 295: 438-446.
16-Perry EK. Medicinal plants and Alzheimer’sdisease: intergrating ethnobutanical andcontemporary scientific evidence. J Altern complement Med 1998; 4(4): 419-427.
17-Fawcett JR, Bordago EZ, Jackson K, Liu H, Peterson J, Suitak A, "et al" .Inactivation of the human brain muscarinic acetylcholinereceptor by oxidative damage. Catalyzed by a low molecular weight endogenous inhibitorfrom Alzheimer’s brain is prevented by pyrophosphate analogos, bioflavonoids andother antioxidant. Brain Res 2002; 950: 10-20.