Determination of Aflatoxin M1 in Fresh (Raw and Pasteurized) Cow Milk in Khuzestan Province

Document Type : Original Article


1 Department of Laboratory Sciences, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.

2 Department of Nursing and Midwifery ,College of Nursing and Midwifery, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran


Background and Objective: Aflatoxin M1 is a life-threatened toxin with high carcinogenicity potency which is secreted in milk by ingestion of contaminated feed in livestock. This study was done to evaluate the level of aflatoxin M1 in raw and pasteurized milk during summer and winter seasons in Khuzestan province.
Subjects and Methods: One hundred and twenty samples (60 pasteurized milk, 60 raw milk (were collected from milk collecting centers and supermarkets during summer and winter of 2011 and AFM1 was evaluated by HPLC method and (P< 0.05) was considered significant.
Results: All raw and pasteurized milk samples were contaminated in winter. The main ± SD of raw and pasteurized milk were 0.013±0.009ng/ml and 0.017±0.0015ng/ml in winter, respectively. The mean ± SD of raw and pasteurized milk were 0.007±0.0012 and 0.009±0.0011 ng/ml, in summer, respectively. The mean concentration of raw and pasteurized milk in summer showed significantly lower levels than in winter (P<0.05). AFM1 was greater than maximum acceptable limit in one pasteurized milk sample with 0.065ng/ml.
Conclusion: With regard to contamination of all samples in winter, fungal growth condition and AFB1 production should be eliminated


1-Celik TH, Sarmehmetoğlu B, Kǘplǘlǘ O. Aflatoxin M1 contamination in pasteurised milk. Veterinarski Arhiv 2005;75(1):57-65.
2-Elgebri AM, Aidoo KE, Candlish AA, Tester RF. Occurrence of aflatoxin M1 in randomly selected North African milk and  cheese samples. Food Addit Contam 2004;21(6):592-7.
3-Codex Alimentarius Publications. Code of Practice for the Reduction of Aflatoxin B1 in Raw Materials and Supplemental Feeding Staffs for Milk Producing Animals. CAC/RCP. Available From: URL: www.Codex - 045e.pdf. Accessed month 6, day 20, year 2003.
4-Zinedine A, Gonzalez-Osnaya L, Soriano JM, Molto JC, Idrissi L, Manes J.  Presence of aflatoxin M1 in pasteurized milk from Morocco. Int J Food Micro 2007;114(1):25-9.
5-Ghanem I, Orfi M. Aflatoxin M1 in raw, pasteurized and powdered milk available in the Syrian market. Food Control 2009;20(6):603- 5.
6-Di Natale F, Gallo M, Nigro R. Adsorbents selection for aflatoxins removal in bovine milks. J Food Eng 2009;95(1):186-91.
7-Kamkar A. A study on the occurrence of aflatoxin M1 in raw milk produced in Sarab city of Iran. Food control 2005;16(7):593-599.
8-Da Silva J B, Dilkin P, Fonseca H, Correa B. Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum. Braz J Microbiol 2004;35(3):182-186.
9-Tajkarimi M , Aliabadi Sh F, Salah Nejad A, Poursoltani H, Motallebi AA,  Mahdavi H. Aflatoxin M1 contamination in winter and summer milk in 14 states in Iran. Food Control 2008;19(11):1033-6.
10-PournoorMohammadi SH, Ansari M, Nezakati Alfati L, Kazemipour M, Hasibi M. [Determination of Aflatoxin M1 in pasteurized milk Consumed in Kerman province]. J Kerman Univ Med Sci 2010;16(3):271-80. [In Persian]
11-Sefidgar SAA, Mirzae M, Assmar M, Naddaf SR. Aflatoxin M1 in pasteurized milk in Babol city, Mazandaran Province, Iran. Iran J Public Health 2011;40(1):115-18.
12-Riazipour M, Tavakkoli HR, Razzaghi Abyaneh M, Rafati H, Sadr Momtaz SM. [Measuring the amount of M1Aflatoxin in pasteurized milks]. Kowsar Med J 2010;15(2):89-93. [In Persian]
13-Lopez CE, Ramos LL, Ramadan SS, Bulacio LC. Presence of aflatoxin M1 in milk for human consumption in Argentina. Food Control 2003;14(1):31-4.
14-Ghazani MH. Aflatoxin M1 contamination in pasteurized milk in Tabriz (northwest of Iran). Food Chem Toxicol 2009;47(7):1624-5.
15-Nazari A, Nourouzi H, Movahedi M, Khaksarian M. [ Measurement of aflatoxin m1 in raw and pasteurized cow milk samples by HPLC]. Yafteh 2007;9(3):49-57 [In Persian]
16-Roussi V, Govaris A, Varagouli A, Botsoglou NA. Occurrence of aflatoxin M1 in raw and market milk commercialized in Greece. Food Addit Contam 2002;19(9):863-8.
17-Alborzi S, Pourabbas B, Rashidi M, Astaneh B. Aflatoxin M1 contamination in pasteurized milk in Shiraz (South of Iran). Food Control 2006;17(7):582-4.
18-Gholampour Azizi I, Khoshnevis SH, Hashemi SJ. [Aflatoxin M1 level in pasteurized and sterilized milk of Babol city]. Tehran Univ Med Sci 2007;65(13):20-4. [In Persian]
19-O’keeffe M, Kennedy O, Farreell F, Dooley M, Grad PB, Nuget A. et .al. Food residue database. Ashtown: The National Food Centers Dunsinea; Dublin and johnstoun Castle Research Centre,Castlenock. wexford year2001. p. 14-16.