Detection of pap, fim, sfa and afa Genes in Eschreshia Coli Strains Isolated from Patients with Urinary Tract Infection by Multiplex-PCR in Kerman, Iran

Document Type : Original Article

Authors

1 MSc Department of Microbiology.Department of Microbiology, School of Basic Sciences, Sirjan Branch, Islamic Azad University, Sirjan, Iran.

2 Assistant Professor of Microbiology. Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Background and Objective: Urinary tract infection is the second most common infection in the human body. Among the causes of urinary tract infections, E. coli is one of the most common urinary tract pathogens in both genders.
Subjects and Methods: In this study, 200 urine samples to detect E. coli were cultured. Isolates were identified by standard biochemical tests and DNA extracted from isolates confirmed to determine the genes papEF, fimH, afaBC and sfa/focDE by Multiplex-PCR (Multiplex-polymerase chain reaction) method.
Results: In total, 110 E. coli strains from urine samples cultured were identified. The genotype virulence genes showed that 42.5 % of the isolates at least one of the four acuity type gene. PapEF gene with 21.81 % was the most frequent gene. While sfa and focDE genes had 4.54 and 2.77 % frequencies respectively. Thirty two samples (29.09%) had both fimH and afaBC genes.
Conclusion: Urinary tract infections are considered as a widespread health concern that is consistent with different geographic regions. The reason for the difference in results with other studies can be due to the difference in sample isolation regions.

Keywords

References

 
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Jundishapur Scientific Medical Journal
Volume 16, Issue 4 - Serial Number 109
September and October 2017
Pages 393-400

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History

  • Receive Date: 16 May 2016
  • Revise Date: 09 August 2017
  • Accept Date: 12 August 2017

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