Association Study of the VDR rs731236 and rs7975232 Polymorphysms with MS in Population of Khuzestan Province

Document Type : Original Article

Authors

1 Master of Science in Genetics.Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 1Department of Genetics, Faculty of science, ShahidChamran University of Ahwaz, Ahwaz, Iran.

3 Molecular Geneticist.Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

4 Associate Professor of Epidemiology. Department of Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Background and Objecitve: Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system (CNS). MS is determined by interaction between genes and environment. In the past decade, much attention has been given to vitamin D and its role in MS. Recent data implicate vitamin D in modulation of the risk as well as clinical course of disease. Since vitamin D شزof the risk as well as clinical course of disease. Since vitamin De in MS. recent D acts through the vitamin D receptor (VDR) the aim of our study was to determine the association between the VDR rs731236 and rs7975232 polymorphisms and the MS risk in population of Khuzestan province.
Subjects and Methods: This study comprised 150 MS cases, plus 150 healthy individuals as a control group. For all subjects, level of vitamin D in serum was measured and association of the VDR rs731236 and rs7975232 polymorphisms and the MS risk was determined using PCR amplification and then RFLP. Data was confirmed by direct sequencing. Statistical analyses were performed using SPSS version 16.0.
Results: Level of vitamin D in serum of case and control groups were not differed. The rs731236 polymorphism odds ratios for AA and GG genotypes were 1.2 (P=0.46) and 1.51 (P=0.33) compared with the AG genotype, respectively. The rs7975232 (C/A) polymorphism odds ratios for CA and AA genotypes were 8.65 (P=<0.001) and 1.38 (P=<0.001) compared with the CC genotype, respectively.
Conclusion: The rs7975232 polymorphism in VDR gene did not show association with MS, but rs7975232 polymorphism showed good association with this disease.

Keywords

Full Text

Background and Objecitve: Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system (CNS). MS is determined by interaction between genes and environment. In the past decade, much attention has been given to vitamin D and its role in MS. Recent data implicate vitamin D in modulation of the risk as well as clinical course of disease. Since vitamin D شزof the risk as well as clinical course of disease. Since vitamin De in MS. recent D acts through the vitamin D receptor (VDR) the aim of our study was to determine the association between the VDR rs731236 and rs7975232 polymorphisms and the MS risk in population of Khuzestan province.

Subjects and Methods: This study comprised 150 MS cases, plus 150 healthy individuals as a control group. For all subjects, level of vitamin D in serum was measured and association of the VDR rs731236 and rs7975232 polymorphisms and the MS risk was determined using PCR amplification and then RFLP. Data was confirmed by direct sequencing. Statistical analyses were performed using SPSS version 16.0.

Results: Level of vitamin D in serum of case and control groups were not differed. The rs731236 polymorphism odds ratios for AA and GG genotypes were 1.2 (P=0.46) and 1.51 (P=0.33) compared with the AG genotype, respectively. The rs7975232 (C/A) polymorphism odds ratios for CA and AA genotypes were 8.65 (P=<0.001) and 1.38 (P=<0.001) compared with the CC genotype, respectively.

Conclusion: The rs7975232 polymorphism in VDR gene did not show association with MS, but rs7975232 polymorphism showed good association with this disease.

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Jundishapur Scientific Medical Journal
Volume 16, Issue 4 - Serial Number 109
September and October 2017
Pages 455-465

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History

  • Receive Date: 21 November 2016
  • Revise Date: 12 June 2017
  • Accept Date: 05 July 2017

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