Protective Effect of Hydroalcoholic Extract of Hawthorn Fruit on CaCl2-Induced Arrhythmias in Rat

Document Type : Original Article


Department of Physiology, Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.


Background and Objective: Antioxidant agents play an important role in prevention and protection in heart diseases, therefore, are used as medicinal plants in traditional medicine. Hawthorn fruit extract, with strong antioxidant properties, has been used effecively in treatment of many diseases. The aim of this study was to investigatetheprotective effect of hydroalcoholic extract ofHawthorn fruit on CaCI2-induced arrhythmias in rat.
Subjects and Methods: Male Sprague Dawley rats divided in control, amiodarone (10 mg/kg, iv) and Hawthorn-extract groups receiving either saline solution (1ml/kg/day, by gavage) or hawthorn extract (100 and 200 mg/kg/day, by gavage) respectively for 10 days. Both groups before experiments and 10 days later lead II electrocardiogram was recorded for calculating electrophysiological parameters such as QRS complex. CaCI2-induced arrhythmia was used for evaluation of possible antiarrhythmic calcium antagonistic effect. The arrhythmia was produced by i.v. injection of a CaCl2 2.5% solution (140 mg/kg) and percentage of ventricular premature beats (VPB), ventricular fibrillation (VF)  and ventricular tachycardia (VT) were recorded in hawthorn groups and was compared to control and amiodarone (10 mg/kg, iv) groups. Results were analyzed by using one-way ANOVA, Fisher and student t-test. P<0.05 was considered as significant level.
Results: The inotropic and chronotropic effect was not significant with hawthorn extract after 10 days administration. Hawthorn extract displayed antidysrhythmic effects on CaCl2-induced arrhythmia compared to control group by significant reduction of VPB, VF and V.tac, comparable to that of amiodarone.
Conclusion: The results of present study such as significant decrease in VPB, VF and V.tac in hawthorn groups showed antiarrhythmic properties of hawthorn fruit extract as a preconditioning agent and suggest the protective effect of hawthorn extract in heart disease comparable to amiodarone.


1-Resnekov L. Cardiac Arryhthmia. Postgrad Med J 1964;40 (465):381-92.
2-Fogoros   RN. Antiarrhythmic drugs: a practical guide. 2nd ed. Malden: Blackwell Futura; 2007. P. 36-51.
3-Khori V, Nayebpour M. [Effect of artemisia absinthium on electrophysiological properties of isolated heart of rats]. Physiol Pharmacol 2007; 10 (4):303-11. [In Persian]
4-Veldkamp MW, Verkerk AO, van Ginneken AC, Baartscheer A, Schumacher C, de Jonge N, et al. Norepinephrine induces action potential prolongation and early afterdepolarizations in ventricular myocytes isolated from human end-stage failing hearts. Eur Heart J 2001; 22 (11):955-63.
5-Trevore AJ, Katzung BG, Masters SB. Katzung & Trevor's Pharmacology Examination & Board Review. 9th ed. New York: Mc Graw-Hill; 2010.
6-Evangelista S, Maggi CA, Meli A. The role of the local anaesthetic properties of beta-adrenoceptor blocking agents in antagonizing CaCl2-induced arrhythmias in the rat. Br J Pharmacol 1981; 73 (3):725–7.
7-Khori V, Naebpour M, Mir-Abbasi A. Ouabaine like effects of ethanolic extract of aerial parts of urtica dioica l. on isolated rabbit atrioventricular node: potential role as an anti – arrhythmic. J Med Plants 2008; 7 (26):24-32. [In Persian]
8-Roughani M, Baluchnejadmojarad T, Roughani Dehkordi F. The effect of Crataegus SPP branchlet feeding on endotheliumdependent contractile and relaxatory response of thoracic aorta from diabetic rats. Modarres J Med Sci 2009;12(2):61-71. [In Persian]
9-Rigelsky JM, Sweet BV. Hawthorn: pharmacology and therapeutic uses. Am J Health Syst Pharm 2002;59(5):417-22.

10-Verma SK, Jain V, Verma D, Khamesra R. Crataegus Oxyacantha - A Cardioprotective Herb. J Herb Med Toxicol 2007;1(1):65-71.

11-Tadić VM, Dobrić S, Marković GM, Dordević SM, Arsić IA, Menković NR, et al. Anti-inflammatory, gastroprotective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract. J Agric Food Chem 2008;56(17):7700-9.

12-Cui T, Nakamura K, Tian S, Kayahara H, Tian YL. Polyphenolic content and physiological activities of Chinese hawthorn extracts. Biosci Biotechnol Biochem 2006;70(12):2948-56.
13-Zhang Z, Ho WK, Huang Y, James AE, Lam LW, Chen ZY. Hawthorn fruit is hypolipidemic in rabbits fed a high cholesterol diet. J Nutr 2002;132(1):5-10.
14-Chang WT, Dao J, Shao ZH. Hawthorn: potential roles in cardiovascular disease. Am J Chin Med 2005;33(1):1-10.
15-Khori V, Azadbakht M, Nayebpour M, Jamshidi AH, Pourabouk M, Mohammad Alizadeh A, et al. [Frequency-dependent anti arrhythmic effects of Crataegus monogyna on the extracellular field potential recordings in the rabbit atrioventricular node, an experimental model of AF]. Physiol Pharmacol 2009;15(1):36-46. [In Persian]

16-Somova LI, Shode FO, Mipando M. Cardiotonic and antidysrhythmic effects of oleanolic and ursolic acids, methyl maslinate and uvaol. Phytomedicine 2004;11(2-3):121-9.

17-Schüssler M, Hölzl J, Fricke U. Myocardial effects of flavonoids from Crataegus species. Arzneimittelforschung 1995;45 (8):842-5.
18-Habs M. Prospective, comparative cohort studies and their contribution to the benefit assessments of therapeutic options: heart failure treatment with and without Hawthorn special extract WS 1442. Forsch Komplementarmed Klass Naturheilkd 2004;11 Suppl 1:36-9.
20-Koch E, Malek FA. Standardized extracts from hawthorn leaves and flowers in the treatment of cardiovascular disorders--preclinical and clinical studies. Planta Med 2011;77(11):1123-8.
21-Nattel S, Davies M, Quantz M. The antiarrhythmic efficacy of amiodarone and desethylamiodarone, alone and in combination, in dogs with acute myocardial infarction. Circulation 1988;77(1):200-8.
Volume 12, Issue 6 - Serial Number 87
July and August 2013
Pages 693-703
  • Receive Date: 04 September 2013
  • Revise Date: 23 September 2013
  • Accept Date: 29 September 2013