The Effect of Two Weight Loss Protocols on Plasma Concentration of Agouti Related Peptide (AgRP) in Elite Wrestlers

Document Type : Original Article

Authors

1 Department of Exercise Physiology, Faculty of Physical education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Exercise Physiology, Faculty of Physical education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

3 Faculty of Sport Sciences, Shahid Beheshti University, Tehran, Iran.

Abstract

Background and Objective: AgRP is a powerful orexigenic peptide which is secreted by the arcuate nucleus of the hypothalamus, and plays an important role in weight control.  The aim of the present study was to compare the effect of two weight loss protocols (1.a researcher recommended weight loss protocol, 2.Traditional weight loss protocol) on resting plasma concentration of AgRP in elit wrestlers.
Subjects and Methods: Thirty elite wrestlers (age 24.33± 3.33 years, BMI 24.90±2.38 Kg.m2 and body fat percentage 12.92±3.01) were randomly divided into three groups (two experimental groups and one control group). Experimental group I: used a twelve-day diet protocol which was suggested by researchers, along with three wrestling exercise sessions on a week. Experimental group ІІ: used traditional diet protocol and sauna, and control group was without diet protocol. Blood samples were collected before and 12-hours after the protocols. Subject’s percent of body fat was measured using the 3-point method.
Results: Both the experimental groups had a significant weight reduction (P>0.001). The experimental groupIshowedsignificantlyreductionin percent of body fat (P>0.001). The levels of plasma AgRP increased in the experimental group II (P>0.01).
Conclusion: The twelve-day weight loss protocol is more effective  than the traditional weight loss protocol. Not only it did not have a significant effect on AgRP level, it also decreased body fat percentage.

Keywords


1-Schwartz MW. Brain pathways controlling food intake and body weight. Exp Biol Med (Maywood) 2001;226(11):978–81.
2-Shutter JR, Graham M, Kinsey AC, Scully SS, Lüthy R, Stark KL. Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice. Genes Dev 1997;11(5):593–602.
3- Pritchard LE, White A. Agouti-related protein: more than a melanocortin-4 receptor antagonist? Peptides 2005;26(10):1759–70
4-Inui A. Transgenic approach to the study of body weight regulation. Pharmacol Rev 2000;52(1):35-61.
5- Oh KW, Lee WY, Rhee EJ, Baek KH, Yoon KH, Kang MI, et al. The relationship between serum resistin, leptin, adiponectin, ghrelin levels and bone mineral density in middle-aged men. Clin Endocrinol (Oxf) 2005;63(2):131–8.
6- Speakman JR. Obesity: the integrated roles of environment and genetics. J Nutr 2004;134(8):2090s-105.
7-Schwartz MW, Woods SC, Porte D Jr, Seeley RJ,  Baskin DG. Central nervous system control of food intake. Nature 2000;404(6778):661-71.
8-Levin BE, Dunn-Meynell AA. Chronic exercise lowers the defended body weight gain and adiposity in diet-induced obese rats. Am J Physiol Regul Integr Comp Physiol 2004;286(4):R771-8.
9-Ghanbari-Niaki A, Nabatchian S, Hedayati M. Plasma agouti-related protein (AGRP), growth hormone, insulin responses to a single circuit-resistance exercise in male college students. Peptides 2007;28(5):1035-9.
10-Ghanbari-Niaki A, Saghebjoo M, Rashid-Lamir A, Fathi R, Kraeme RR. Acute circuit-resistance exercise increases expression of lymphocyte agouti-related protein in young women. Exp Biol Med (Maywood) 2010;235(3):326-34.
11-Delphan M, Rashidlamir A, Delphan F. Resting plasma AgRP levels response to exercise-conjugated diet and only diet in overweight and obese sedentary females. Biol Sport 2012;29(1):45-50.
12-Rashidlamir A, Ghanbari-niaki A. Effect of 8-week circuit training on lymphocyte AGRP gene expression in well-trained wrestlers. Daneshvar Med 2010;17(89):62-77. [In Persian]
13-Hosseini Kakhak SA, Niaki A, Ghanbari Rahbarizade F, Mohagheghi MA, Khabazian MB, Fathi R, et al. [The effect of treadmill running on plasma and muscle agouti-related protein (AgRP) concentration in male rats]. Iran J Endocrinol Metab 2009;11(4):455-61. [In Persian]
14- Rodriguez NR, Di Marco NM, Langley S: American College of Sports Medicine position stand. Nutrition and athletic performance. Med Sci Sports Exerc 2009;41(3):709-31.
15-Loucks AB. Energy balance and body composition in sports and exercise. J Sports Sci 2004;22(1):1–14.
16-Katsuki A, Sumida Y, Gabazza EC, Murashima S, Tanaka T, Furuta M, et al. Plasma levels of agout-irelated protein are increased in obese men. J Clin Endocrinol Metab 2001;86(5):1921–4.
17-Rashidlamir A, Goodarzi M, Ravasi A. The comparison of acute and gradual weight loss methods in well-trained wrestlers. World J Sport Sci 2009;2(4):236-40.
18-Wanger DR. Body composition assessment and minimal weight recommendations for high school wrestlers. J Athl Train 1996;31(3):262-5.
19-Dill DB, Costill DL. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 1974;37(2):247-248.
 20-Leibowitz SF, Wortley KE. Hypothalamic control of energy balance: different peptides, different functions. Peptides 2004;25(3):473–504.
21-de Rijke CE, Hillebrand JJ, Verhagen LA, Roeling TA, Adan RA. Hypothalamic neuropeptide expression following chronic food restriction in sedentary and wheel-running rats. J Mol Endocrinol 2005;35(2):381-90.
22-Yu Y, Deng C, Huang XF. Obese reversal by a chronic energy restricted diet leaves an increased Arc NPY/AgRP, but no alteration in POMC/CART, mRNA expression in diet-induced obese mice. Behav Brain Res 2009;205(1):50–6.
23-Remmers F, Delemarre-van de Waal HA. Developmental programming of energy balance and its hypothalamic regulation. Endocr Rev 2011;32(2):272-311.
24-Katsuki A, Sumida Y, Gabazza EC, Murashima S, Tanaka T, Furuta M, et al. Plasam levels of agouti-related protein are increased in obese men. J Clin Endocrinol Metab 2001;86(5):1921-4.
25-Timpmann S, Ööpik V, Pääsuke M, Medijainen L, Ereline J. Acute effects of self-selected regimen of rapid body mass loss in combat sports athletes. J Sports Sci Med 2008;7(2):210-7.
26-Petrie HJ, Stover EA, Horswill CA. Nutritional concerns for the child and adolescent competitor. Nutrition 2004;20(7-8):620–31. .c resincluded 30 remaidedمتوسط ، 2/0 تا 4/0 به معنای توافق ضعیف و کمتر از 2/0 به معنای توافق ناچیز در نظر گرفته شد.