Isolation and Determination of Hypericins from Different Parts of Hypericum triquetrifolium Turra Grown in Khozestan by UV/Visible Spectrophotometry

Document Type : Original Article

Authors

1 Associate Professor of Pharmacognosy.Department of Pharmacognosy, Medicinal Plant Research Center,School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Assistant Professor of Medicinal Chemistry.Department of Medicinal Chemistry, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Pharmacist.Pharmacist.

Abstract

Background & Objective:Hypericin is a naphtodianthrone with multiple pharmacologic actions. Hypericum triquetrifolium Turra grows in different part of Iran. The aim of the present study was to isolate and determine the quantity of hypericins in different parts (leaf, flower and stem) of this plant grown in Khozestan province.
Matrials & Methods: Differentparts of Hypericum triquetrifolium Turra (leaf, flower and stem) were collected and extracted with methanol 50% using soxhlet apparatus. Hypericin was determined using UV/Visible spectrophotometer at 590 nm.
Results: The percents of hypericin in leaves, flowers and stems were 310, 210 and 150 mg/100g, respectively.
Conclusion: Leaves and flowers of Hypericum triquetrifolium Turra have considerable amounts of hypericin.
 

Keywords

References

 
1-Ghahraman A. Color Atlas of Iranian Flora. Research Institute of Forest and Rangelands publishing; 1996. p. 3071.
2-Mozaffarian V. A dictionary of Iranian plant names. Tehran: Farhang Moaser; 1996. p. 137.
3-Xu Y,Lu C. Raman spectroscopic study on structure of human immunodeficiency virus (HIV) and hypericin-induced photosensitive damage of HIV. Sci China C Life Sci 2005;48:117-32.
4-Miskorsty P. Hypericin--a new antiviral and antitumor photosensitizer: mechanism of action and interaction with biological macromolecules. Curr Drug Targets 2002;3:55-84.
5-Blank M, Lavie G, Mandel M, Hazan S, Orenstein A, Meruelo D, et al. Antimetastatic activity of the photodynamic agent hypericin in the dark. Int J Cancer 2004; 111:596-603.
6-Reichling J, Weseler A, Saller R. A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry2001;34:116-8.
7-Carpenter S, Fehr MJ, Kraus GA, Petrich JW.Chemiluminescent activation of the antiviral activity of hypericin: a molecular flashlight. Proc Natl Acad Sci U S A 1994;91: 12273-7.
8-Jacobson JM, Feinman L, Liebes L, Ostrow N, Koslowski V, Tobia A, et al. Pharmacokinetics, safety, and antiviral effects of hypericin, a derivative of St. John's wort plant, in patients with chronic hepatitis C virus infection. Antimicrob Agents Chemoter 2001;45:517-24.
9-Sarris J, Kavanagh DJ. Kava and St. John's Wort: current evidence for use in mood and anxiety disorders. J Altern Complement Med 2009;15:827-36.
10-Couladis M, Baziou P, Verykokidou E, Loukis A. Antioxidant activity of polyphenols from Hypericum triquetrifolium Turra. Phytother Res 2002;16:769–70.
11-Apaydin S, Zeybek U, Ince I, Elgin G, Karamenderes C, Ozturk B, et al. Hypericum triquetrifolium Turra. extract exhibits antinociceptive activity in the mouse. J Ethnopharmacol 1999;67:307–12.
12-Bruni R, Sacchetti G. Factors affecting polyphenol biosynthesis in wild and field grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae). Molecules 2009;14:682-725.
13-Knox JP, Dodge AD. Isolation and activity of the photodynamic pigment hypericin. Plant Cell Environment 1985;8:19-25.
14-Ayan AK, Cirak C. Variation of hypericins in Hypericum triquetrifolium Turra growing in different locations of Turkey during plant growth. Nat Prod Res 2008;22:1597-1604.
15-Xenophontos M, Stavropoulos E, Avramakis E, Navakoudis E, Dornemann D, Kotzabasis K. Influence of the developmental stage on the (proto)–hypericin and (proto) pseudohypericin levels of Hypericum plants from Crete. Planta Med 2007;73:1309-15.
16-Alali F, Tawaha K, Al-Eleimat T. Determination of hypericin content in Hypericum triquetrifolium Turra (Hypericaceae) growing wild in Jorden . Nat Prod Res 2004;18:147-51.
Jundishapur Scientific Medical Journal
Volume 11, Issue 3 - Serial Number 78
July and August 2017
Pages 285-294

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History

  • Receive Date: 28 April 2010
  • Revise Date: 04 May 2011
  • Accept Date: 21 January 2012

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