Effects of Forced Exercise on Withdrawal Syndrome, Brain Hippocampus Neurons Count and Level of Serum Corticosterone in Morphine Addicted Male Rats

Authors

1 Professor of Physiology.Physiology Research Center & Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 M.Sc. Student in Physiology.Department of Physiology & Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Associate Professor of Anatomy. Department of Anatomy & Physiology Research Center & Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Assistant Professor of Physiology. Department of Physiology & Faculty of Medicine, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

5 M.Sc. of Bioanformatic.Physiology Research Center & Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background and Objective: addiction to morphine impairs the behavioral and cognitive performances. The aim of the present study was to evaluate the effects of forced exercise (treadmill) on withdrawal signs after morphine deprivation, serum corticostrone level,and hippocampusneurons count in brain hemisphers in rats addicted to morphine.
Subjects and Methods: Adult male Wistar rats were divided into five groups with 10 in each: 1) exercised control (C+E), 2) sham exercised control (C+Sh.E), 3) addicted (A), 4) exercised addicted (A+E), and 5) sham-exercised addicted (A+Sh.E). Withdrawal signs such as number of jumping, teeth chattering, wet-dog shaking, defecation, body scratching, and standingas number of were counted during 30 minutes after naloxone administration. Animals in exercised groups ran on treadmill one hour daily from 9-10 Am in the morning for ten consecutive  days. Sham-exercised groups passed same times on turned off treadmill while its shock delivered system was turned on. At the end of experiments serum corticostrone level and hippocampus neurons count were done after decapitation the animals in all groups. The data were analyzed with one-way ANOVA that followed by LSD post hoc test. The differences between groups were accepted as significant with P value less than 0.05.
Results: Addiction to morphine increased withdrawal signs and corticostrone secretion significantly and reduced hipocampal neurons in brain, All off which were significant. Forced exercise could inhibit certain withdrawal signs induced by morphine deprivation in addicted rats while could not reverse increased corticostrone level and decreased hippocampus neurons.
Conclusion: Despite of useful effects of forced exercise on health conditions and especially cognition during aging, it would cause impair severely some neurobehavioral and hormonal disorders in addicted rats to morphine.

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 11, Issue 1 - Serial Number 76
July and August 2002
Pages 11-25

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History

  • Receive Date: 27 July 2011
  • Revise Date: 29 November 2011
  • Accept Date: 21 December 2011

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