Effect of Low-calorie Diet Supplemented with Genistein on Lipid Profile, Blood Glucose and Proinflammatory Biomarkers in Diet-induced Obese Rats

Authors

1 M.Sc. of Nutrition. Department of Nutrition, Faculty of Paramedicine, Nutrition Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Assistant Professor of Nutrition.Department of Nutrition, Faculty of Paramedicine, Nutrition Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Associate Professor of Nutrition. Department of Nutrition, Faculty of Paramedicine, Diabetes Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background and Objective: Obesity is a worldwide public health problem that results in comorbidities including diabetes, dyslipidemia, coronary artery disease and some types of cancer. It seems that soy isoflavones can improve obesity and reverse subsequent metabolic disorders. In this study, we assessed the effect of restriction of calorie supplemented with genistein on diet-induced obese rats.
Subjects and Methods: Thirty rats obesed with high fat diet were divided randomly into 3 experimental groups (n=10) as follows: group 1: low calorie diet supplemented with 50mg/kgbw genistein, group2: low calorie diet supplemented with Dimethyl Sulphoxide (DMSO) (as vehicle) and group3: obese control rats with ad libitum access to standard food. After 4 weeks, fasting blood samples were collected and analyzed for biochemical analysis.
Results: The results showed that administration of genistein in conjunction with low calorie diet can synergistically improve triglyceride (p=0.005), VLDL (p=0.005), total cholesterol(p=0.002) and LDL-C (P=0.003) and increase HDL-C (p=0.001) but has no effect on body weight and proinflammatory biomarkers (resistin and CRP). Restriction of calorie also resulted in the decrease of glucose level, TG, TC, LDL, proinflammatory biomarker (CRP) and increase in HDL-C (p<0.05) but has no effect on resistin level.
Conclusion: It seems that administration of genistein with restriction of calorie is useful for improvement of hyperlipidemia in obese hyperlipidemic patients.
Sci Med J 2012;11(1):57-67

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 11, Issue 1 - Serial Number 76
July and August 2002
Pages 57-67

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History

  • Receive Date: 15 October 2011
  • Revise Date: 25 January 2012
  • Accept Date: 04 February 2012

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