Evaluation of Nanohydroxyapatite Powder Synthesized by Sol-gel Combustion Method and Bio Oss on Reconstruction of Parietal Bone Defects in Rat

Document Type : Original Article


1 Department of Physics, Faculty of Science, Shahid Chamran University, Ahvaz, Iran.

2 Department of Periodontology. Dental School, Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Department of Anatomy. Medical School, Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Department of Periodontology. Dental School, Jundishapur University of Medical Sciences, Ahvaz, Iran.


Background and Objective: The aim of this research is to investigate the possible use of hydroxyapatite nanoparticles in parietal bone regeneration.
Subjects and Methods: Ca10(PO4)6(OH)2 nanoparticles were synthesized by sol-gel combustion method using citric acid as combustion agent. The calcium nitrate tetrahydrate, citric acid and ammonium dihydrogen phosphate and deionized water with the proper stoichiometry were used. The  nanoparticles were studied by the TG/DTA, XRD, FT-IR, SEM and TEM techniques. XRD. Fourier transform infrared spectroscopy (FT-IR) was used to identify different bond groups in the structure of the samples. The hydroxyapatite nanopowders were used on of adult male Sprague Dawley rats for parietal bone regeneration.
Results: The product had a hexagonal structure. SEM images showed morphology and mean nanoparticles size.
Conclusion: The results showed that the bone repairing with HA nanoparticles occur similar to typical repairing by commercially available (Bio Oss) and even it takes place with minimal tissue inflammation.


1-Bogdanoviciene I, Beganskiene A, Tõnsuaadu K, Glaser J, Meyer H J, Kareiva A. Calcium hydroxyapatite, Ca10(PO4)6(OH)2 ceramics prepared by aqueous sol–gel processing. Mater Res Bull 2006; 41(9):1754–62.
2-Shojai MS. Hydroxyapatite. Tehran: Iranian Students Book Agency; 2010. p. 45-6. [In Persian]
3-Rhee SH. Synthesis of hydroxyapatite via mechanochemical treatment. Biomaterials 2002; 23(4):1147–52. 
4-Han Y, Li S, Wang X, Chen X. Synthesis and sintering of nanocrystalline hydroxyapatite powders by citric acid sol–gel combustion method. Mater Res Bull 2004; 39(1):25–32.
5-Hwang K, Lim Y. Chemical and structural changes of hydroxyapatite films by using a sol–gel method. Surf Coat Tech 1999;115(2):172–5.
6-Goloshchapov DL, Kashkarov VM, Rumyantseva NA, Seredin PV, Lenshin AS, Agapov BL, et al. Synthesis of nanocrystalline hydroxyapatite by precipitation using Hen's eggshell. Ceram Int 2013; 39(4):4539-49.
7-Yuan-yuan Z, Jie T, Ying-chun P, Wei W, Tao W. Electrochemical deposition of hydroxyapatite coatings on titanium. Trans Nonferrous Met SOC China 2006;16(1):633-7.
8-Nathanael AJ, Arul NS, Ponpandian N, Mangalaraj D, Chen PC. Nanostructured leaf like hydroxyapatite/TiO2 composite coatings by simple sol–gel method. Thin Solid Films 2010; 518(24):7333–8.
10-Saha SK, Banerjee A, Banerjee S, Bose S. Synthesis of nanocrystalline hydroxyapatite using surfactant template systems: Role of templates in controlling morphology. Mat Sci Eng 2009; 29(7):2294–301. 
11-Kumar GS, Girija EK, Thamizhavel A, Yokogawa Y, Kalkura SN. ­Synthesis and characterization of bioactive hydroxyapatite–calcite nanocomposite for biomedical applications. J Colloid Interface Sci 2010; 349(1):56–62.
12-Guo X, Xiao P. Effects of solvents on properties of nanocrystalline hydroxyapatite produced from hydrothermal process. J Eur Ceram Soc 2006; 26(15):3383–91.
13-Milev A, Kannangara GSK, Ben-Nissan­ B. Morphological stability of hydroxyapatite precursor. Mater Lett 2003;57(13-14):1960–5.
14-Islam M, Mishra PC, Patel R. ­Physicochemical characterization of hydroxyapatite and its application towards removal of nitrate from water. J Environ Manage 2010; 91(9):1883–91.