The Effect of Deprivation of Food and Water on Formation of Urinary Crystals in Rat’s Kidney as an Experimental Model

Document Type : Original Article


1 Assistant Professor of Urology.Department of Urology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Resident of Urology,Department of Urology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Clinical Pathologist.Department of Immunology, Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.


Backgroundand Objective:During the holy month of Ramadan, the number of admissions of patients with renal colic increases. This study was conducted to investigate the effects deprivation of food and water on the urinary crystals formation in the kidneys of rats as an experimental model of Ramadan-type fasting (RTF).
Subjects and Methods: In this experimental study, 32 male Wistar rats were randomly allocated into four groups. The first (Ct) and the third (EG) groups of the rats received standard pellet diet and water ad libitum. The second (RTF) and the fourth (RTF+EG) groups of the animals were fasted (12 h) and then re-fed (12 h) daily. Drinking water containing 0.75% ethylene glycol were given to EG and RTF+EG groups. The existence and the frequency of crystal deposition of calcium oxalate in the renal tissue stained by Hematoxylin and Eosin (HE) were observed in each group by light microscopy. Frequency of crystal deposition on 10 fields (×20) was calculated for each group. The results are expressed as Mean±SEM and also was graded semi quantitatively in five categories as (-), (+), (++), (+++), and (++++), respectively.
Results: Oxalate calcium crystals were not observed in Ct and RTF groups. The Mean±SEM of crystal deposition in the EG and RTF+EG groups were 4.9±0.29 and 4.2±0.24, respectively. There was no statistically significant difference between the two groups.
Conclusions: The results of this study showed that there are not significant associations between deprivation of food and water with formation as well as amount of calcium oxalate deposition in kidney.


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