A Phytochemical Study and Comparison of the Effect of Pomegranate Extracts Spongy Tissue on Colon Cancer Cells Caco-2

Document Type : Original Article


1 Assistant Professor of Biology.Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Associated Professor of Chemistry.Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 M.Sc of Organic Chemistry.Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.


Background and Objective: The goal of this studying was evaluation of anticancer and anti-oxidant properties of four types of extracts from Pomegranate (Punica granatum L) spongy tissue on colon cancer cells Caco-2.
Subjects and Methods: The phytochemicals of spongy tissues of pomegranate were identified by phytochemical methods. In order to evaluate cytotoxic and anti-oxidant characteristics, ethanolic and n-hexane extracts from pomegranate spongy tissues were collected, using Soxhlet apparatus and maceration methods. The effects of these extracts on Caco-2 colon cancer cells were evaluated using MTT, NBT and TBA tests, as well as catalase enzyme levels.
Results: The photochemical analysis confirmed the presence of flavonoids, tannin, alkaloid, terpenoides, aldehyde, ketones and vitamin C compounds, but no steroids, saponins and protein were detected. The results of MTT showed the least cell survival value of treated cancerous cell was obtained with n-hexane-derived extract extract using Soxhlet method. Furthermore, NBT results demonstrated that the n-hexane extract using Soxhlet method exhibited peroxidant characteristics and droves the cancerous cells toward apoptosis. On the other hand, TBA results showed that the highest cytotoxicity belonged to n-hexane extract by Soxhlet and ethanolic extract by maceration method respectively. The highest catalase enzyme activity was observed in treatment by maceration ethanolic extract.
Conclusion: The existence of several compounds with pro-apoptotic properties in the extracts of spongy tissues of pomegranate propose the notion that using the compounds of these extracts alone or in combination with chemotherapy may be beneficial in treatment of colon cancer.


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