The Effect of Aqueous and Methanolic Extract of Cyperus Rotundus Rhizome on Insulin Secretion from Pancreatic Islets in Male Mouse

Document Type : Original Article

Authors

1 Department of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Department of Physiology, School of Medicine, Member of Student Research Committee of Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.

Abstract

Background and Objectives: According to traditional medicine as hypoglycemic Cyperus-Rotundus-rhizome, the aim of this study was to investigate the effect of this plant on insulin secretion from the islets of Langerhans isolated male mouse.
Subjects and Methods: This experimental study was carried out on 90 adult Nmari male mice (20-25gr). Pancreatic islets of Langerhans from intact mice were isolated by collagenase digestion method. Then aqueous and methanol extract of plant at 0.1, 0.5 and 1% concentrations and also glyburide at 1 and 10μM applied on isolated islets in culture medium containing different glucose concentrations (2.8,5.6 and 16.7mM). Insulin secretion from islets was evaluated in incubation system and insulin was measured using an ELISA kit. Data analyzed using spss14 and ANOVA test.
Result: Insulin secretion significantly increased at 16.7 mM glucose concentration compared with 2.8 mM concentration (p<0.05). In 2.8 and 5.6 mM glucose, glyburide 10μM could increase insulin secretion significantly (p<0.001). Aqueous extract (0.05 and 0.1 percent) in the presence of glucose 2.8 (p<0.001) and 5.6mM (p<0.05) caused the significant increase in insulin secretion, but in the presence of high glucose a significant reduction in insulin production was seen. The results showed that the methanolic extract couldn’t increase the amount of insulin secretion, significantly.
Conclusion: The results of this study showed beneficial effects of lower doses aqueous extract of Cyperus-Rotundus-rhizome are the increased secretion of insulin. Probably this plant through effects on insulin secretion islets and, of course, at low doses, can be effective in the treatment of diabetes.
 

Keywords

References

1-Ashok k, tiwari J Madhusudana Rao. Diabetes Mellitus therapeutic of phytochemical: present status and future prospects. Current science. 2002; 283: 30-38
2-Daily G. New strategies for basal insulin treatment in type 2 diabetes mellitus. Clin. Ther.2004; 6: 889–901.
3-Katzung BG, Basic and Clinhcal Pharmacology, In: Martha S. Nolte Kennedy. Pancreatic hormons and antidiabetic drugs. 12th New York: Lang Medical Books/McGraw-Hill; 2012: p. 743-768.
4-Galer BS, Gianas A, Jensen MP. Painful diabetic poly neuropathy: epidemiology pain description and quality of life. Diabetes Res Prac. 2000; 47: 123-128.
5-Badgujar SB, Bandivdekar AH Evaluation of a lactogenic activity of an aqueous extract of Cyperus rotundus Linn.J Ethnopharmacol. 2015; 163:39-42.
6-Ahangarpour A, Yahyavi H. Effect of Cyperus rotundus rhizomes on blood glucose, Lipid, insulin and hepatic enzymes in male rats model of insulin resistance. Qom University of medical sciences Journal. 2011; 5(2(18)):70-75. (Persian)
7-O’Dowd JF. The isolation and purification of rodent pancreatic islets of langerhans. Methods Mol Biol. 2009; 560: 37–42.
8-Ahangarpour A, Eskandari M, Vaezlari.A, Hashhemitabar.M. Effect of aqueous and hydroalcoholic extract of Berberis vulgaris on insulin secetion from islets of langerhans isolated from male mice. YUMSJ. 2012; 17(4): 289-98. (Persian)
9-Ahangarpour A, Heidari H, Mard SA, Hashemitabar M, Khodadadi A. Progesterone and cilostazol protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide. Iran J Pharm Res. 2014; 13(3): 937-44.
11-Itabashi N, Okada K, Muto S, Fujita N, Ohta T, Miyazaki Ji: A novel enhancer of insulinotrophic action by high glucose (JTT-608) stimulates insulin secretion from pancreatic beta-cells via a new cellular mechanism. J Pharmacol Exp Ther. 2001; 297:953-960.
12-Khayatian M, Larijani A, Farzami B, Boushehri H, Pournour Mohamadi SH: Effects of glibenclamide on insulin secretion of Pancreatic islets and glucokinase activity in normal and diabetic rat. Iranian Journal of Diabetes and Lipid Disorders. 2006; 6: 17-26.
13-Raut NA, Gaikwad NJ. Antidiabetic activity of hydro-ethanolic extract of Cyperus rotundus in alloxan induced diabetes in rats. Fitoterapia. 2006; 77: 585–588
14-Mojab F., Vahidi H., Nikavara B., Kamalinezhad M. Chemical components of essential oil and antimicrobial effects of rhizomes from cyperus rotundus L. journal of medicinal plants. 2009; 8(32):91-97.(Persian)
15-Zargari A. Medicinal plants. 7th ed. Tehran: Tehran university; 2002: p. 680-681.
Jundishapur Scientific Medical Journal
Volume 14, Issue 6 - Serial Number 99
January and February 2015
Pages 623-632

Files

History

  • Receive Date: 18 August 2015
  • Revise Date: 14 October 2015
  • Accept Date: 26 October 2015

Share

How to cite

Statistics