Evaluation of Salivary Melatonin in Patients with Oral Lichen Planus Referring to Dentistry Faculty of Jundishapur Ahvaz University at 2011-2012

Document Type : Original Article


1 Department of Oral and Dental Diseases, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Postgraduate Student of Pediatric Dentistry

3 Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Department of Orthodontics, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.

5 Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.


Background and Objective: Oral Lichen Planus (OLP) is a chronic and precancerous disease. The imbalances between   the levels of free radical and antioxidants may play an important role in the onset and development of several inflammatory oral pathologies such as Lichen Planus. Melatonin as an antioxidant can play a role in scavenging free radicals and prevent OLP conversion to cancer. The aim of this study was to evaluate melatonin level in patients with OLP.
Subjects and Methods: In this analytical studytwenty two patients diagnosed with OLP, were recruited as patients group, and twenty age- and sex-matched healthy volunteers were recruited as control group. Melatonin levels in whole unstimulate saliva (WUS) was determined by ELISA test. Statistically analyzed was carried out with LSD and Duncan test.
Result: The mean saliva melatonin levels in patients with OLP was lower than those of healthy control group, but it was not statistically significant (P=0.3). The mean saliva melatonin levels in patients with reticular type of OLP compared with control group was significantly decreased (P<0.05) but in  erythematose/ulcerative type was not statistically significant (P>0.05).
Conclusion: The findings of this study demonstrated that the level of melatonin was decreased in patients with Licben planus, and supports the notion that more attention to treatment of this much neglected disease need to be made. These findings also suggest that the low level of this antioxidant may lead to more destructive injury and put the patients at risk of development of oral cancer..  


AghaHosseini F, MirzaiiDizgah I, Mikaili S, Abdollahi M. Increased salivary lipid peroxidation in human subjects with oral lichen planus. Int J Dent Hyg 2009 Nov; 7(4): 246-50.
2-Sezer E, Ozugurlu F, Ozyurt H, Sahin S,  Etikan I. Lipid peroxidation and antioxidant status in lichen planus. Clin Exp Dermatol 2007Jul; 32(4): 430-4.
3-Greenberg MS, Glick M, Ship JA. Burket oral medicine diagnosis and treatment. 11th ed. Hamilton: BC Decker; 2008. P. 104-10.
4-Scully C, Carrozzo M. Oral mucosal disease: Lichen planus. Br J Oral Maxillofac Surg 2008Jan; 46(1):15-21.
5- Eisen D, Carrozzo M, Bagan Sebastian J-V, Thongprasom K. Oral lichen planus: clinical features and management. Oral Diseases 2005Nov; 11(6): 338-49.
6-Neville BE, Damm DD, Allen CM, Bouqot JE. Oral maxillofacial pathology. 3rd ed. Phiadelphia: WB Saunders; 2009. P. 782-5.
7-Regezi JA, Sciubba JJ, Jordan RCK. Oral pathology: clinical pathologic correlation. 5th ed. Philadelphia: WB sanders, 2008. P. 90-1.
8-Vitorino R, Lobo MJ, Ferrer-Correira AJ, Dubin JR, Tomer KB, Domingues PM, "et al". Identification of human whole saliva protein components using proteomics. Proteomics 2004Apr; 4(4): 1109 -15.
 9-Dawes C. Salivary flow patterns and the health of hard and soft oral tissues. J Am Dent Assoc 2008 May; 139 (Suppl1): 18s -24.
10-Pedersen AM, Bardow A, Beier Jensen S, Nauntofte B. Saliva and gastrointestinal functions of taste, mastication, swallowing and digestion. Oral Diseases 2002 May; 8(3): 117-29.
11-Mese H,  Mastuo R. Salivary secretion, taste and hyposalivation. J Oral Rehabil 2007; 34(10): 711–23.
12-De Almedia PVD, Gregio AMT, Machado MAN, De Lima AAS, Azevedo LR. Saliva composition and functions. J Contemp Dent Pract 2008 Mar; (9)3: 72-80.
13- Llena-Puy C. The rôle of saliva in maintaining oral health and as an aid to diagnosis. Med Oral Patol Oral Cir Bucal 2006 Aug; 11(5): 449-55.
14-Pfaffe T, Cooper-White J,  Beyerlein P, Kostner K. Diagnostic Potential of Saliva: Current State and Future Applications. Clin Chem 2011May; 57(5): 675-87.
15-Bowman J, Miller C, Campbell C, Bailey A, Floriano PN, Christodoulides N, "et al". Translational research: next generation salivary biomarker cardiovascular devices at point of care. 87th ed. Miami, Florida: International Association of Dental Research/American Association of Dental Research/Canadian Association of Dental Research; 2009. P. 25-50.
16-Hofman F. Human Saliva as a Diagnostic Specimen.  J Nutr 2001May; 131(5): 1621-5.
17-Jung B, Ahmad N. Melatonin in Cancer Management.Cancer Res 2006Oct; 66(20): 9789-93.
18-Czesnikiewicz-Guzik M, Konturek SJ, Loster B, Wisniewska G, Majewski S. Melatonin and its role in oxidative stress related diseases of oral cavity. J Physiol Pharmacol 2007Aug; 58(Supl3): 5-19.
19-Barikbin B, Yousefi M, Rahimi H, Hedayati M, Razavi SM, Lotfi S. Antioxidant status in patients with lichen planus. Clin Exp Dermatol 2011Dec; 36(8): 851-4.
20-Cutando A, Galindo P, Gómez-Moreno G, Arana C, Bolaños J, Acuña-Castroviejo D, "et al". Relationship between salivary  melatonin and severity of periodontal disease. J periodontal 2006 Sep; 77(9): 1533-7.
21-Battino M, Bullon P, Wilson M, Newman H. Oxidative injury and inflammatory periodontal diseases: the challenge of antioxidants tofree radicals and reactive oxygen species. Crit Rev Oral Biol Med1999; 10(4): 458-476.
22-Upadhyay RB, Carnelio S, Shenoy RP, Gyawali P, Mukherjee M. Oxidative stress and antioxidant defense in oral lichen planus and oral lichenoid reaction. Scand J Clin Lab Invest 2010 Jul; 70(4): 225-8.