The Comparison of Effect of Chlorhexidine and Proanthocyanidin and Grape Seed Extract on Composite-Dentin Micro Tensile Bond Strength: an in Vitro Experimental Study

Document Type : Original Article


1 Assistant Professor of Odontología Operativa.-Department of Odontología Operativa, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Assistant Professor of Odontología Operativa.Department of Odontología Operativa, Faculty of Dentistry, University of Medical Sciences Birjand, Birjand, Iran.

3 Resident of Odontología Operativa.Department of Odontología Operativa, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Assistant Professor of Farmacognosia.Department of Farmacognosia, Faculty of Farmaci, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.


Background and Objective: Achieving a strong bond between composite resin and dentin remains a challenge in restorative dentistry. The aim of this study was to evaluate the effect of a 2% chlorhexidine (as a matrix metalloproteinase inhibitor), grape seed extract and proanthocyanidin (as a cross-linking agents to further stabilize collagen fibrils) on the dentin bond strength.
Subjects and Methods: The occlusal enamel was removed from 35 human sound premolars and dentin surfaces etched with phosphoric acid and washed with water. Teeth were allocated into seven groups: chlorhexidine, grape seed extract, proanthocyanidin, chlorhexidine plus grape seed extract, chlorhexidine plus proanthocyanidin, ethanol (the solvent of grape seed extract), and left unexposed to any solution (control). Then, a resin composite restoration was made on the dentin surface after using etch-and-rinse adhesive.
The micro tensile bond strength (µTBS) of the specimens was analyzed after three month of storage in distilled water and 1500 thermocycles.
Results: Multivariate analysis of variance and t-test showed that control group had a higher bond strength and chlorhexidine plus grape seed extract group had a lowest bond strength than another groups. According to variance analysis chlorhexidine and grape seed extract and proanthocyanidin had comprising effect on dentin bond strength.
Conclusion: Use of chlorhexidin, grape seed extract and proanthocyanidin did not increase the composite-dentine micro tensile bond strength.

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