Prevalence of Carbapenem-Resistant Klebsiella pneumoniae in Patients Admitted to Taleghani Hospital of Abadan During 2014-2015


1 Department of Microbiology, School of Medical, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Department of Microbiology, International Branch of Arvand, Ahvaz Jundishapur University of Medical Sciences, Abadan, Iran.


Background and Objectives:  Metallo-beta-lactamases (MBLs), are enzymes that hydrolyze penicillins, cephalosporins and carbapenems. Nowadays, the carbapenem-resistant Klebsiella pneumoniae is considered as one of the most important causes of nosocomial infections and has created various health problems in the world. The purpose of this study was to determine the prevalence of MBL producing Klebsiella pneumoniae and blakpc gene in clinical samples taken from patients referred to Taleghani hospital of Abadan in the period 2014-2015.
Subjects and Methods: Detection of Klebsiella pneumoniae colonies resistance to the common nine-antibiotics was performed by disk diffusion test, according to CLSI protocols. Identification of MBL was carried out using IPM- EDTA combined disk method. Finally, the blakpc gene produced by the isolated strains was identified using PCR method and specific primers.
Results: Among 144 cases of isolated Klebsiella bacteria, the highest and lowest frequencies of resistance in Klebsiella pneumoniae are related to antibiotic amoxicillin (127 cases, 88.1%) and amikacin (13 cases, 9.2%) respectively. The results of the combined disk method showed that 27 isolates (18.75%) of all samples are MBL producing, from which 14 cases (51.8%) showed the presence of blakpc gene. Most of the isolates were separated from urine samples of patients admitted to ICU and urology department.
Conclusion: The results of this study demonstrated that the emergence of MBLs and detection of the responsible gene in Klebsiella pneumoniae isolates among our patients is a warning finding and warrants to change the pattern of use of antibiotics in order to prevent the spread of such infections in hospitalized patients.


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