Comparison of the Effect of Using Chlorhexidine Solutionon Microleakage of Two Pit and Fissure Sealants


1 Faculty of Dentistry, Jundishapur University of Medical Sciences, Ahwaz, Iran

2 Department of Pedodontics, Faculty of Dentistry, Jundishapur University of Medical Sciences, Ahwaz, Iran.


Background and Objectives: Marginal seal of the materials is extremely important in sealant therapy. The aim of this study was to compare the effect of chlorhexidine onmicroleak age of two materials used as pit-and-fissure sealants.
Subjects and Methods: The occlusal surface of sixty extracted non-carious molars were cleaned, etched with 38% phosphoric acid, rinsed and dried. The teeth were randomly divided in 4 groups, Group A: Bonding agent (DenTASTIC UNO™)+flowable composite (FLOWS-RITE™ ), Group B: Bonding agent + fissure sealant (SEAL-RITE™), Group C: chlorhexidine 2% (Consepsis®) + bonding agent + Flowable composite, Group D: Chlorhexidine 2% + bonding agent + fissure sealant. Following sealant placement, the teeth were thermocycled,then coated with two layers of nail lac, leaving exposed a 1.5-mm window around the sealant margins. Finally the specimens were immersed in 2% buffered methylene blue for 4 hours, sectioned longitudinally in a buccolingual direction and analyzed for leakage using a stereomicroscope. A 4-criteria ranked scale was used to score dye penetration and data were analyzed statistically using the Kruskal-Wallis and Mann-Whitney tests.
Results: All materials exhibited dye penetration to some extension and no statistically significant difference was observed among the groups (P>0.05).
Conclusion: The findings of this study showed that flowable composite resin had similar marginal seal as the conventional sealant. In addition, using chlorhexidine 2% prior to sealant placement seems to have no adverse effect on sealing ability of pits and fissure materials; however, further in vitro and in vivo studies are necessary.


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