Comparison of the Effect of Using Chlorhexidine Solutionon Microleakage of Two Pit and Fissure Sealants

Authors

1 Faculty of Dentistry, Jundishapur University of Medical Sciences, Ahwaz, Iran

2 Department of Pedodontics, Faculty of Dentistry, Jundishapur University of Medical Sciences, Ahwaz, Iran.

Abstract

Background and Objectives: Marginal seal of the materials is extremely important in sealant therapy. The aim of this study was to compare the effect of chlorhexidine onmicroleak age of two materials used as pit-and-fissure sealants.
Subjects and Methods: The occlusal surface of sixty extracted non-carious molars were cleaned, etched with 38% phosphoric acid, rinsed and dried. The teeth were randomly divided in 4 groups, Group A: Bonding agent (DenTASTIC UNO™)+flowable composite (FLOWS-RITE™ ), Group B: Bonding agent + fissure sealant (SEAL-RITE™), Group C: chlorhexidine 2% (Consepsis®) + bonding agent + Flowable composite, Group D: Chlorhexidine 2% + bonding agent + fissure sealant. Following sealant placement, the teeth were thermocycled,then coated with two layers of nail lac, leaving exposed a 1.5-mm window around the sealant margins. Finally the specimens were immersed in 2% buffered methylene blue for 4 hours, sectioned longitudinally in a buccolingual direction and analyzed for leakage using a stereomicroscope. A 4-criteria ranked scale was used to score dye penetration and data were analyzed statistically using the Kruskal-Wallis and Mann-Whitney tests.
Results: All materials exhibited dye penetration to some extension and no statistically significant difference was observed among the groups (P>0.05).
Conclusion: The findings of this study showed that flowable composite resin had similar marginal seal as the conventional sealant. In addition, using chlorhexidine 2% prior to sealant placement seems to have no adverse effect on sealing ability of pits and fissure materials; however, further in vitro and in vivo studies are necessary.
 

Keywords


 
1-Pardi V, Sinhoreti M, Pereira A, Ambrosano G, Meneghim M. In vitro evaluation of microleakage of different materials used as pit-and-fissure sealants. Brazilian dental journal 2006; 17(1): 49–52.
2-Welbury R, Raadal M, Lygidakis N. EAPD guidelines for the use of pit and fissure sealants [Serial Online] 2004; 3:179-184. Available from: http://www.eapd.gr/dat/D052751D/file.pdf
3-Dukić W, Dukić O, Milardović S. The influence of Healozone on microleakage and fissure penetration of different sealing materials. Collegium antropologicum 2009; 33(1): 157–62.
4-Simonsen R. Pit and fissure sealant: review of the literature.Pediatric dentistry 2002; 24(5): 393–414.
5-Beauchamp J, Caufield P, Crall J, Donly K, Feigal R, Gooch B, "et al". Evidence-based clinical recommendations for the use of pit-and-fissure sealants: a report of the American Dental Association Council on Scientific Affairs. Dental clinics of North America 2009;53(1):131–47, x.
6-HevingaM. Management of occlusal caries [Serial Online] 2011. Available from:
7-James P, Parnell C, Whelton H. The caries-preventive effect of chlorhexidine varnish in children and adolescents: a systematic review. Caries research 2010; 44(4): 333–40.
8-Campos E, Correr G, Leonardi D, Pizzatto E, Morais E. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Brazilian oral research 2009; 23(3): 340–5.
9-Hiraishi N, Yiu C, King N, Tay F. Effect of 2% chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements. Journal of dentistry 2009; 37(6): 440-8.
10-Vianna M, Gomes B, Berber V, Zaia A, Ferraz C, de Souza-Filho F. In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2004; 97(1): 79–84.
11-Autio-Gold J. The role of chlorhexidine in caries prevention. Operative dentistry 2008; 33(6): 710–6.
12-Zhang Q, Mulder J, Truin G-J, van PalensteinHelderman W. Effect of 40% chlorhexidine varnish on mutans streptococci counts in pits and fissures of permanent first molars. Journal of dentistry 2007; 35(7): 588–92.
13-Joharji R, Adenubi J. Prevention of pit and fissure caries using an antimicrobial varnish: 9 month clinical evaluation. Journal of dentistry 2001; 29(4): 247–54.
14-Carrilho M, Carvalho R, De Goes M, Di Hipolito V, Geraldeli S, Tay F, "et al". Chlorhexidine preserves dentin bond in vitro. Journal of dental research 2007;86(1):90–94.
15-Chitre S. Evaluation of two methods of fissure treatment before sealant placement on different caries levels [Serial Online] 2009. Available from: http://scholarworks.iupui.edu/bitstream/handle/1805/2078/view.pdf
16-Dukić W, Dukić O, Milardović S, Vindakijević Z. Clinical comparison of flowable composite to other fissure sealing materials--a 12 months study. Collegium antropologicum 2007; 31(4): 1019–24.
17-Toodeshkchooei D, Ahmadi M. In vitro Microleakage Comparison of Two Fissure Sealants and two Flowable Composite Resins. J Dent Shiraz Univ Med Sci 2012; 34(1): 391-7.
18-Zhang Q, van’t Hof M, Truin G, Bronkhorst E, van PalensteinHelderman W. Caries-inhibiting effect of chlorhexidine varnish in pits and fissures. Journal of dental research 2006; 85(5): 469–72.
19-Bahrololoomi Z, Soleymani A, Heydari Z. In vitro comparison of microleakage of two materials used as pit and fissure sealants. Journal of dental research, dental clinics, dental prospects 2011; 5(3): 83–6.

20-Shafiei F, Memarpour M, Khajeh F. The effect of Chlorhexine Disinfectant on Microleakage of Adhesive Systems in Composite Restorations. J Dent Shiraz Univ Med Sci 2010; 11(3): 228-34.

21-Owens B, Lim D, Arheart K. The effect of antimicrobial pre-treatments on the performance of resin composite restorations. Operative dentistry 2003; 28(6): 716–22.

22-Salari B, Shahabi S, Bagheri H, Yousefi M. The effect of three disinfectants (chlorhexidine, sodium hypochlorite and hydrogen peroxide) on the microleakage of 7th generation bonding agents. Journal of Dental Medicine-Tehran University of Medical Sciences 2014; 26(4): 321-27.
23-Kahnamoee M, EbrahimiChaharom M, Kimyai S, Bahari M, Badamchizadeh S. Effect of Cavity Disinfection with Chlorhexidine on Microleakage of Gingival Margin in Cl-V Composite Restorations Restored with One-step Self-etch Adhesive Resin: an in Vitro Study.Medical Journal of Tabriz University of Medical Science & Health Service 2011; 33(3): 47-51.
24-Memarpour M, Shafiei F. The Effect of Antibacterial Agents on Fissure Sealant Microleakage: A 6-month In Vitro Study. [Serial Online] 2014; 12(2): 149-158. Available from: http:// europepmc.org/ abstract/ MED/ 24624384
25-Javadinejad Sh, GhasemiToodeshkiD ,Salehi A. Sealant Microleakage Evaluation of Three Common Fissure Preparation Methods. J Dent Shiraz Univ Med Sci 2011; 12(2): 127-32.