Effect of Angustifolia Aqueous Extract on IL-6 Serum Levels in Mice Receiving Lipopolysaccharide of Brucella abortus

Document Type : Original Article


1 Department of Immunology, Baqiyatallah University of Medical Sciences, Tehran, Iran.

2 Department of Anatomy, Baqiyatallah University of Medical Sciences, Tehran, Iran.

3 Kaka 4-Department of Anatomy, Baqiyatallah University of Medical Sciences, Tehran, Iran

4 Department of Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

5 Department of Microbiology, Baqiyatallah University of Medical Sciences, Tehran, Iran.


Background and Objectives: The incidence of inflammatory  disease  is  increasing around  the world. Conventional therapies in patients with inflammatory diseases unable to cure completely. However, recent studies have shown anti-inflammatory  effects  of angustifolia aqueous  extract. The  IL-6  cytokine  level  is  increased  in many  inflammatory  diseases and is  recognized  as  a  marker  for  inflammatory  diseases. Therefore, this study aimed to evaluate the effect of  angustifolia  aqueous  extract  on  IL-6  serum levels  in  mice receiving lipopolysaccharide  of  Brucella  abortus.
Subjects and Methods: Twenty five mice were divided  into  five equal groups. The control group did not receive the extract. The  four  next  groups  intraperitoneally  received  the different  dose of 10, 20, 30 and 40 mg/kg of angustifolia  aqueous extract daily for four days. All groups intraperitoneally received 100 mg of  lipopolysaccharide of  Brucella  abortus  on  the  fourth  day. Blood samples were collected by cardiac puncture. The sera were separated and kept  frozen  at −80°C  and  thawed shortly before determination  of  IL-6  levels  by  the  ELISA  kit.
Results: Our  results  showed  that  serum  concentrations  of  IL -6 ( pg/ml) in groups receiving angustifolia aqueous  extract  at dose of 10 mg/kg (P<0.05), 20 mg/kg (P = 0.o14), 30 mg/kg (P =0.012) and 40 mg/kg (P=0.023) significantly decreased  when  compared  to  control group (p <0.05).


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