The Comparison of Preventive Effect of Apium Graveolens Hydroalcoholic and Aqueous Extract on Formalin-Induced Inflammation in Male Rat Hind Paw

Document Type : Original Article

Authors

1 Professor of Pharmacology and Toxicology.Department of Pharmacology and Toxicology, School of Pharmacy and Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Assistant Professor of Pharmacology,Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Pharmacy Student,School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background and Objective: Gastrointestinal complications caused by using nonsteroidal anti-inflammatory drugs (NSAIDs) motivated researchers to pay more attention to herbal medicines with anti-inflammatory effect. The aim of this study was to compare the anti-inflammatory effect of hydroalcoholic and aqueous extract of Apium graveolens with aspirin in treatment of formalin-induced inflammation in rat hind paw.
Subjects and Methods:Male Wistar rats (weighting 150-180 g) were used in this study. To prepare hydroalcoholic extract, ethanol (70%) and maceration method were used and to prepare aqueous extract distilled water and maceration method were used. Animals were divided into 11 groups (6 in each). Negative control group received 5ml/kg normal saline and positive control group received 300 mg/kg aspirin. The test groups received various increasing doses of the hydroalcoholic extract (100, 200, 400, 600 mg/kg) or 100, 200, 400, 600, 800 mg/kg of aqueous extract of Apium graveolens via intraperitoneal route. Thirty minutes later all groups received 100 µl formalin 2.5% subcutaneously at the hind paw. Then, changes in the volume of rat paw were measured hourly for 5 hrs using plethysmometer.
Results: All hydroalcoholic extract treated groups showed significant difference (p

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 15, Issue 5 - Serial Number 104
November and December 2016
Pages 497-506

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History

  • Receive Date: 21 June 2016
  • Revise Date: 25 October 2016
  • Accept Date: 01 November 2016

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