Comparison of the Effect of Six Weeks of Continuous and IntervalTraining on Some Indicators of Immune System in Healthy Non-Athlete Males

Document Type : Original Article


1 Department of Physical Education, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

2 Department Exercise Physiology, Faculty of Physical Education and Sport Sciences, ShahidChamran University, Ahvaz, Iran


Background and Objective: The immune system is one of the most important systems in the health setting that influenced by several factors. The purpose of this study was to compare the effect of six weeks of continuous and interval training on some indicators of immune system in healthy non-athlete males.
Subjects and Methods: Among all healthy non-athlete male students of Islamic Azad University of Ahvaz branch, 30 students were selected by randomly sampling style and divided into two groups of 15 persons (continuous and intervaltraining groups). Anthropometry indicators, body mass index, and aerobic capacity (Vo2max) were evaluated at the first session. Interval training group performed of five periods 3-minute exercises on a cycle ergometer with 80% VO2max of intensity and continuous training consisting of a 30-minute of continuous trainingon a cycle ergometer with 60% VO2max of intensity for 6 weeks(Three times a week) . Blood sample were taken before and 24 hr after training to analysis indicators of immune system.
Results: Data analysis indicated that a significant difference was found between number of neutrophils in two groups (P<0.05). However, no significant differences between number of leukocytes, lymphocytes, eosinophils, monocytes and basophils in continuous and interval training groups (P > 0.05).
Conclusion: According to this results, can be concluded that, Whereas significant decrease or increase the number of leukocytes may create risks of inflammation or infection, therefore the training can effect in health of immune and cardiovascular system by maintain the immune function indicators.
Please cite this paper as:
Boostani M, RezaeiNasab H. Comparison of the Effect of Six Weeks of Continuous and IntervalTraining on Some Indicators of Immune System in Healthy Non-Athlete Males.JundishapurSci Med J 2017;16(1):25-34


1-Gleeson M. Immune functions in sport and exercise. Journal of applied physiology. 2007; 103(2):693-9.
2-Wolach B, Gavrieli R, Ben-Dror SG, Zigel L, Eliakim A, Falk B. Transient decrease of neutrophil chemotaxis following aerobic exercise.Medicine and science in sports and exercise. 2005; 37(6):949-54.
3-Wolach B. Exercise and the immune system-Focusing on the effect of exercise on neutrophil functions: Intech Open Access Publisher; 2012.
4-Nieman D, Henson D, Gojanovich G, Davis J, Dumke C, Utter A. Immune changes: 2 h of continuous vs. intermittent cycling. International journal of sports medicine. 2007;28(07):625-30.
5-Henson DA, Nieman DC, Nehlsen-Cannarella SL, Fagoaga OR, Shannon M, Bolton MR. Influence of carbohydrate on cytokine and phagocytic responses to 2 h of rowing. Medicine and science in sports and exercise. 2000;32(8):1384-9.
6-Pedersen BK, Toft AD.Effects of exercise on lymphocytes and cytokines.British Journal of Sports Medicine. 2000;34(4):246-51.
7-Nieman DC.Immune response to heavy exertion.Journal of applied physiology. 1997;82(5):1385-94.
8-Denadai BS, Ortiz MJ, Greco CC, de Mello MT. Interval training at 95% and 100% of the velocity at VO2 max: effects on aerobic physiological indexes and running performance. Applied Physiology, Nutrition, and Metabolism. 2006;31(6):737-43.
9-Avloniti AA, Douda HT, Tokmakidis SP, Kortsaris AH, Papadopoulou EG, Spanoudakis EG. Acute effects of soccer training on white blood cell count in elite female players. International journal of sports physiology and performance. 2007 Sep;2(3):239-49.
10-Church TS, Finley C, Earnest CP, Kampert JB, Gibbons LW, Blair SN. Relative associations of fitness and fatness to fibrinogen, white blood cell count, uric acid and metabolic syndrome. International journal of obesity and related metabolic disorders: journal of the International Association for the Study of Obesity. 2002;26(6):805-13.
11-Shabkhiz F, Ravasi A, Hassan Z, Taghikhani M, Razavi TA.The Effect of Aerobic Continuous and Interval Training and Detraining on Some Indexes of the Cellular Immune System in Female Wistar Rats.World. 2008; 1(1):17-26.
12-Takahashi I, Umeda T, Mashiko T, Chinda D, Oyama T, Sugawara K, et al. Effects of rugby sevens matches on human neutrophil-related non-specific immunity. British journal of sports medicine. 2007;41(1):13-8.
13-Bonsignore M, Morici G, Vignola A, Riccobono L, Bonanno A, Profita M, et al. Increased airway inflammatory cells in endurance athletes: what do they mean? Clinical & Experimental Allergy. 2003;33(1):14-21.
14-Johannsen NM, Priest EL, Dixit VD, Earnest CP, Blair SN, Church TS.Association of white blood cell subfraction concentration with fitness and fatness.British journal of sports medicine.2008 Oct 16.
15-Azizbeigi K, Azarbayjani MA, Peeri M, Agha-Alinejad H, Stannard S. The effect of progressive resistance training on oxidative stress and antioxidant enzyme activity in erythrocytes in untrained men.Int J Sport NutrExercMetab. 2013;23(3):230-8.
16-Arazi H, Damirchi A, Asadi A. Comparison of acute hormonal responses to moderate intensity resistance exercise in young and middle-aged men.Razi Journal of Medical Sciences. 2014;21(118):64-72.
17-Nieman DC. Does Exercise Alter Immune Function and Respiratory Infections? President's Council on Physical Fitness and Sports Research Digest. 2001.
18-Terra R, Silva SAGd, Pinto VS, Dutra PML. Effect of exercise on immune system: response, adaptation and cell signaling. RevistaBrasileira de MedicinadoEsporte. 2012;18(3):208-14.
19-Rana JS, Boekholdt SM, Ridker PM, Jukema JW, Luben R, Bingham SA, Day NE, Wareham NJ, Kastelein JJ, Khaw KT. Differential leucocyte count and the risk of future coronary artery disease in healthy men and women: the Epic-Norfolk Prospective Population Study. Journal of internal medicine. 2007 Dec 1;262(6):678-89.
20-Johannsen NM, Swift DL, Johnson WD, Dixit VD, Earnest CP, Blair SN. Effect of different doses of aerobic exercise on total white blood cell (WBC) and WBC subfraction number in postmenopausal women: results from DREW. PloS one. 2012;7(2):e31319.
21-Deilami A, Sangari M, Shojaeimehr MT.The Effect of Two Intensive Aerobic and Anaerobic Tests on Immune System in Male Athletic Students in Iran.World Applied Sciences Journal. 2013;21(10):1521-9.
22-Mohr M, Draganidis D, Chatzinikolaou A, Barbero-Álvarez JC, Castagna C, Douroudos I, et al. Muscle damage, inflammatory, immune and performance responses to three football games in 1 week in competitive male players. European journal of applied physiology. 2016;116(1):179-93.