Genetic Variation of Salmonella enteritidis Isolated from Kerman Health Centers by ERIC-PCR

Document Type : Original Article


1 Department of Science in Cellular and Molecular Biology, University of Tehran, Tehran, Iran

2 Department of Biology, Faculty of Science, Imam Hossain University, Tehran, Iran


Background and Objective: Salmonellosis is one the most important zoonotic infectious disease. Salmonella enteritidis is one of the most prevalent serotypes that cause gastroenteritis in human. Epidemiological studies and molecular typing of Salmonella strains could improve infectious disease prevention strategies and control measures. The purpose of the present study was evaluation of genetic diversity of Salmonellaenterica serovar enteritidis isolated from human samples by ERIC-PCR method.
Subjects and Methods: In this cross sectional study, Salmonella strainswere isolated from patients referred to health centers in Kerman. Enteritidis strains was identified by standard microbiological, biochemistry and serotyping methods. Molecular typing of strains was evaluated by ERIC-PCR method.
Results: Forty eight of isolated samples were identified as S. enteritidis. ERIC-PCR analysis differentiated strains into 13 clusters (E1-E13).  Eighteen strains (37.5%) with the same pattern were clustered in E9 and E13.
Conclusion: The results showed that Salmonella enteritidis strains are genetically heterogeneous and could be attributed to diverse ERIC clusters. The results demonstrated that ERIC-PCR is a proper approach for genotypic discrimination of  S. enteritidis isolates.
Please cite this paper as:
Moghadam A, Nazarian Sh.Evaluation of Genetic Variation of Salmonella enteritidis Isolated from Kerman Health Centers by ERIC-PCR
Jundishapur Sci Med J 2017;16(1):53-63


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