Evaluation of SLC30A8 gene polymorphism (rs13266634) distribution in patients with diabetes type 2 in west Mazandaran - Iran.

Shokrzadeh, Mohammad; Mohammad Pour, Abbas; Gerami, Mahyar; Daryaei, Mostafa; Abbasi Roshan, Mehdi; Rajabi Sahne Saraee, Mehran

doi:10.22118/jsmj.2018.106381.1206

Evaluation of SLC30A8 gene polymorphism (rs13266634) distribution in patients with diabetes type 2 in west Mazandaran - Iran.

Document Type : Original Article

Authors

mohammad shokrzadeh ¹

abbas mohammad pour ²

mahyar gerami ³

mostafa daryaei ⁴

mehdi abbasi roshan ⁵

mehran rajabi sahne saraee ⁵

¹ Associate Professor, Pharmacutical Research Center,Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

² Assistant Professor, Pharmacutical Research Center,Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

³ Assistant Professor of Plant Physiology - University of sana- mazandaran-sari –iran .

⁴ M.S – Genetics - University of sana- mazandaran-sari –iran .

⁵ Graduate Student of Genetics , Sana non-profit institution, Sari, Iran.

10.22118/jsmj.2018.106381.1206

Abstract

Background and Purpose: type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion.The SLC30A8 gene is considered as one of the most important candidate genes of type 2 diabetes.
analysis method:In this case-control study, 135 patients and 76 healthy subjects were contributed. 5cc peripheral blood of diabetics and healthy subjects (controls) was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and polymorphism genotype was investigated using PCR-RFLP method.
Results:Genotype and allele frequencies of the rs13266634 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0/0003 and P = 0/0664 , respectively). The frequency of C allele was 89% in type 2 diabetes group and 67% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 10/0, 95% CI 1/9124 – 591/3058 , 2/924 – 924/239)
Conclusion:Our results confirm the association between the rs13266634 polymorphism of the SLC30A8 gene and the increased risk of type 2 diabetes in the west Mazandaran papulation.

Keywords

Type 2 diabete

rs13266634 polymorphism

SLC30A8 gene

PCR-RFLP