1
Diabetes Research Center,Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2
medical chemistry department, factuly of pharmacy, ahvaz jundishapur university medical sciences, iran
3
medical genetic department, factuly of Medicine, ahvaz jundishapur universy medical sciences, ahvaz, iran
10.22118/jsmj.2018.118676.1281
Abstract
abstract Background and Objective: In this study, a high-performance liquid chromatography method was developed to determine the concentration of gliclazide in plasma. The Photodiode Array (PDA) detector allows for the determination of purity. Subjects and Methods: The mobile phase used in this study was phosphate buffer with pH = 3.5 and acetonitrile ( HPLC grade) at a ratio of 1: 1 was run isocraticaly through a C18 analytical column. The UV detection was done at 253 nm. Analytical run time was less than 10 min Results: In the optimum conditions, the calibration curve in the area of 50 to 400 ng / ml, linear with the equation y = 28.029x + 212.74 and 0.998 = R2. Gliclazide is detected within 3.6 minutes by photodiode detector. The limit of detection (LOD) and the limit of quantitative (LOQ) in this method are calculated as 23 ng / ml and 71 ng / ml, respectively. Conclusion: The evaluation of the accuracy and precision of method showed that this method has the ability to determine the amount of gliclazide in plasma, and the method is reliable and fast.
saberi,M. , ramezani,Z. , saberi,A. and rashidi,H. (2018). Identification and quantification of gliclazide in human plasma by HPLC-PDA. (e237178). Jundishapur Scientific Medical Journal, 17(2), e237178 doi: 10.22118/jsmj.2018.118676.1281
MLA
saberi,M. , , ramezani,Z. , , saberi,A. , and rashidi,H. . "Identification and quantification of gliclazide in human plasma by HPLC-PDA" .e237178 , Jundishapur Scientific Medical Journal, 17, 2, 2018, e237178. doi: 10.22118/jsmj.2018.118676.1281
HARVARD
saberi M., ramezani Z., saberi A., rashidi H. (2018). 'Identification and quantification of gliclazide in human plasma by HPLC-PDA', Jundishapur Scientific Medical Journal, 17(2), e237178. doi: 10.22118/jsmj.2018.118676.1281
CHICAGO
M. saberi, Z. ramezani, A. saberi and H. rashidi, "Identification and quantification of gliclazide in human plasma by HPLC-PDA," Jundishapur Scientific Medical Journal, 17 2 (2018): e237178, doi: 10.22118/jsmj.2018.118676.1281
VANCOUVER
saberi M., ramezani Z., saberi A., rashidi H. Identification and quantification of gliclazide in human plasma by HPLC-PDA. Jundishapur Scientific Medical Journal, 2018; 17(2): e237178. doi: 10.22118/jsmj.2018.118676.1281
