Investigation of MDM2 gene promoter polymorphism (SNP309) in patients with acute myeloid leukemia in Khuzestan province

Rostami, Elham; Mohamadi, Mohamad; Bagheri, Shahram

doi:10.22118/jsmj.2018.108567.1220

Investigation of MDM2 gene promoter polymorphism (SNP309) in patients with acute myeloid leukemia in Khuzestan province

Document Type : Original Article

Authors

elham Rostami ¹

mohamad mohamadi ²

shahram bagheri ³

¹ Chamran

² Shahid Chamran University

³ Jundishapur University of Medical Sciences

10.22118/jsmj.2018.108567.1220

Abstract

Acute myeloid disease is a genetic disorder resulting from a change in the natural mechanisms of the blood precursor cells such as proliferation and differentiation. Mouse Double Minute 2 (MDM2) is an oncoprotein with E3 ubiquitin ligase activity that acts as a potent negative regulator for P53. The most well-known MDM2 gene polymorphism is SNP309 polymorphism. Substitution of G nucleotide instead of the T at 309 position increases the expression level of the MDM2 gene. Increasing the MDM2 level decrease the activity of p53 which can lead to cancer.
method:
This study was performed on 91 AML blood samples and 115 control blood samples in Khuzestan province. Different genotypes of 309 position of MDM2 genes were determined using PCR-RFLP and Sequeing method.
Results and Conclusion:
The results show that there is statistically significant association between MDM2 SNP309 polymorphism and AML. In this study the frequency of TT, TG, and GG genotypes was 15%, 41% and 43% in AML group and 62%, 22% and 14% in control group respectively. The genotypic distribution of this polymorphism is associated with AML.
Key words: Leukemia, Polymorphism, MDM2 gene

Keywords

lukemia"

"polymorfism"

"gene"