Background and aim: Sleep is a reversible state in which the capacity for reaction and interaction with the environment is limited and consciousness is lost. 5HT3 receptors are mainly expressed in hippocampus CA1 regions, plays a vital role in sleep regulation and memory consolidation. The present study aims to investigate the effects of activation and inactivation of serotonin 5HT3 receptor by MCHL and Y25130 on acquired memory impairment induced by total sleep deprivation and REM sleep deprivation induction in mice, respectively.Method: Water box apparatus was used to induce total sleep deprivation, multi-platform apparatus was applied to induce RSD. Passive avoidance memory test was used to evaluate memory consolidation Results: Doses of 0.01 and 0.001 mg MCHL and 0.1 mg Y25130 dose significantly reduced the acquired memory (P <0.01). While MCHL at 0.1 and also doses of 0.1 and 0.01 Y25130 significantly increased motor activities (P <0.01), however, both drugs did not significantly affect the pain in all doses. TSD and RSD- induced amnesia improves by Y25130 with sub- threshold dose of significantly (P <0.01). While both drugs had no significant effect on motor activity in TSD and RSD conditions (P> 0.05). But, MCHL under RSD conditions and Y25130 under TSD conditions significantly increased analgesia (P <0.01(.
Conclusion: According to our findings, it seems that CA1 5HT3receptors play a critical role in cognitive and non-cognitive behaviors induced by TSD and RSD.