Jundishapur Scientific Medical Journal

Jundishapur Scientific Medical Journal

The Effect of Eight Weeks of Endurance Training Supplemented by Pumpkin Seed Extract on Oxidative Stress Indicators and DNA Damage in Kidney Tissues of Male Rats Toxcicated with Hydrogen Peroxide

Document Type : Original Article

Authors
Fatemeh Zirrahian 1
Tahereh Bagherpoor 2
Nematollah Nemati 2
Vida Hojati 3
1 PhD Student, Department of Physical Education and Sports Science, Da.C., Islamic Azad University Damghan, Iran
2 Assistant professor, Department of Physical Education and Sports Science, Da.C., Islamic Azad University Damghan, Iran
3 Assistant professor, Department of Biology, Da.C., Islamic Azad University, Damghan, Iran
10.22118/jsmj.2025.508565.3840
Abstract
Background and Objectives Physical training and nutrition may have a protective role in the kidney through their anti-inflammatory effects. The aim of the present study was to investigate the effect of eight weeks of endurance training combined with pumpkin seed extract on DNA damage in the kidney tissue of male rats toxicated with hydrogen peroxide.
Subjects and Methods In the present experimental study, 48 male Wistar rats were divided into healthy control groups, hydrogen peroxide poisoned control, 1 g and 2 g pumpkin seed extract per kilogram of body weight, endurance training, and endurance training combined with 1 g and 2 g pumpkin seed extract per kilogram of body weight.
Results Hydrogen peroxide induction caused a significant decrease in adenosine triphosphate (ATP) (P=0.001) and a significant increase in 6-methylglutamine (P=0.001), malondialdehyde (MDA) (P=0.001) and peroxidase-antioxidant balance (PAB) (P=0.001) in kidney tissue. A net effect of exercise (P=0.001), a net effect of supplementation (P=0.001) and an interaction effect of exercise+supplementation (P=0.039) were observed on the concentration of ATP and PAB in kidney tissue. A net effect of exercise (P=0.001) and a net effect of supplementation (P=0.001) were observed on the concentration of 6-methylguanine and MDA in kidney tissue, but the interaction effect of exercise+ supplementation was not significant (P=0.391).
Conclusion Based on the findings of the present study, endurance training and pumpkin seed extract can provide renal protection by improving antioxidant status.
Keywords
Kidney
Oxidative stress
Endurance training
Pumpkin
Subjects
1.      Jafari M, Motamedi P, Khaledi N. The Effect of Chronic Kidney Disease Induction Upon Affective Factors on Muscle Apoptosis in Rats and the Role of Aerobic Training in Its Modulation. Jundishapur Scientific Medical Journal. 2021 Sep 23;20(4):382-9.
2.     Gyurászová M, Gurecká R, Bábíčková J, Tóthová Ľ. Oxidative stress in the pathophysiology of kidney disease: implications for noninvasive monitoring and identification of biomarkers. Oxidative medicine and cellular longevity. 2020;2020(1):5478708.[ 10.1155/2020/5478708 ][PMID]
3.     Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochimica et Biophysica Acta (BBA)-General Subjects. 2014 Sep 1;1840(9):2709-29.[ 10.1016/j.bbagen.2014.05.017 ][PMID]
4.     Foladi F, Azarbayjani MA, Peeri M, Ghazalian F. The effect of Purslane seed supplement and aerobic training on mitochondrial biogenesis markers and DNA damage in kidney tissue in rats toxicated by hydrogen peroxide.
5.     Madany J. Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract. Polish journal of veterinary sciences. 2016. [10.1515/pjvs-2016-0054 ][PMID]
6.     Asghari S, Azarbayjani MA, Maghsoud P, Homaee HM. The effect of pumpkin seeds hydroalcoholic extract and endurance training on mitochondrial biogenesis markers and DNA damage in ovarian tissue in rats toxicated by hydrogen peroxide.
7.     Magherini F, Fiaschi T, Marzocchini R, Mannelli M, Gamberi T, Modesti PA, Modesti A. Oxidative stress in exercise training: The involvement of inflammation and peripheral signals. Free radical research. 2019 Dec 2;53(11-12):1155-65. [10.1080/10715762.2019.1697438 ][PMID]
8.     Siu PM, Pei XM, Teng BT, Benzie IF, Ying M, Wong SH. Habitual exercise increases resistance of lymphocytes to oxidant‐induced DNA damage by upregulating expression of antioxidant and DNA repairing enzymes. Experimental physiology. 2011 Sep 1;96(9):889-906. [10.1113/expphysiol.2011.058396 ][PMID]
9.     Fairus AM, Choudhary B, Hosahalli S, Kavitha N, Shatrah O. Dihydroorotate dehydrogenase (DHODH) inhibitors affect ATP depletion, endogenous ROS and mediate S-phase arrest in breast cancer cells. Biochimie. 2017 Apr 1;135:154-63. [10.1016/j.biochi.2017.02.003 ][PMID]
10.  Gökbel H. Acute exercise induced oxidative stress and antioxidant changes. European Journal of General Medicine. 2006;3(3):126-31. [][PMID]
11.  Kawamura T, Muraoka I. Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants. 2018 Sep 5;7(9):119. [10.3390/antiox7090119 ][PMID]
12.  Jafari M, GHALAVAND A, RAJABI H, KHALEDI N, MOTAMEDI P. A review of the effect of exercise training on neuromuscular junction in throughout life: A logical analysis of animal experimental studies.
13.  Ghalavand A, Fathi K, Ghobadi MR, Jafari M, Moslehi M, Mafakher L, Zeighami F. The role of physical activity in modulating COVID-19 neuropsychological complications: a narrative review.
14.  Mas-Bargues C, Escriva C, Dromant M, Borras C, Vina J. Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. Archives of biochemistry and biophysics. 2021 Sep 30;709:108941. [10.1016/j.abb.2021.108941 ][PMID]
15.  Algul S, Ugras S, Kara M. Comparative evaluation of MDA levels during aerobic exercise in young trained and sedentary male subjects. Eastern Journal of Medicine. 2018 Apr 1;23(2):98.
16.  Meng Q, Su CH. The impact of physical exercise on oxidative and nitrosative stress: balancing the benefits and risks. Antioxidants. 2024 May 7;13(5):573. [10.3390/antiox13050573 ][PMID]
17.  Ye B, Wang J, Zhou L, Yu X, Sui Q. Perfluoroalkyl acid precursors in agricultural soil-plant systems: Occurrence, uptake, and biotransformation. Science of The Total Environment. 2024 Feb 20;912:168974. [10.1016/j.scitotenv.2023.168974 ][PMID]
18.  Zirrahian F, Bagherpoor T, Nemati N, Hojati V. Investigate the role of supplements of pumpkin seeds and exercise activity on oxidative stress factors. EBNESINA. 2020 Jun 10;22(2):36-43. [][PMID]
19.  Shiri E, Rahnema M, Bigdeli MR. The effect of pumpkin seed oil (Cucurbita moschata) on the permeability of the blood-brain barrier and on brain edema in stroke animal model.
20.  Abou Seif HS. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-suef University Journal of basic and applied sciences. 2014 Sep 1;3(3):178-85.
21.  Kumar S, Srivastava N, Gomes J. The effect of lovastatin on oxidative stress and antioxidant enzymes in hydrogen peroxide intoxicated rat. Food and Chemical Toxicology. 2011 Apr 1;49(4):898-902. [10.1016/j.fct.2010.12.014 ][PMID]
22.  Sedigheh A, Jamal MS, Mahbubeh S, Somayeh K, Mahmoud RK, Azadeh A, Fatemeh S. Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. African Journal of Pharmacy and Pharmacology. 2011 Dec 22;5(23):2620-6.
23.  Mallikarjuna K, Shanmugam KR, Nishanth K, Wu MC, Hou CW, Kuo CH, Reddy KS. Alcohol-induced deterioration in primary antioxidant and glutathione family enzymes reversed by exercise training in the liver of old rats. Alcohol. 2010 Sep 1;44(6):523-9. [10.1016/j.alcohol.2010.07.004 ][PMID]
24.  Chang B, Yi XJ. The Effects of Acute Swimming on Lipid Peroxidation, ATP Synthesis Activity and Total Calcium of Rats' Kidney Mitochondria. In2009 ETP International Conference on Future Computer and Communication 2009 Jun 6 (pp. 258-261). IEEE.
25.  Kestenbaum B, Gamboa J, Liu S, Ali AS, Shankland E, Jue T, Giulivi C, Smith LR, Himmelfarb J, de Boer IH, Conley K. Impaired skeletal muscle mitochondrial bioenergetics and physical performance in chronic kidney disease. JCI insight. 2020 Mar 12;5(5):e133289. [10.1172/jci.insight.133289 ][PMID]
26.  Tamaki M, Miyashita K, Wakino S, Mitsuishi M, Hayashi K, Itoh H. Chronic kidney disease reduces muscle mitochondria and exercise endurance and its exacerbation by dietary protein through inactivation of pyruvate dehydrogenase. Kidney international. 2014 Jun 1;85(6):1330-9. [10.1038/ki.2013.473 ][PMID]
27.  Gastin PB. Energy system interaction and relative contribution during maximal exercise. Sports medicine. 2001 Aug;31(10):725-41. [10.2165/00007256-200131100-00003 ][PMID]
28.  Wang SY, Huang WC, Liu CC, Wang MF, Ho CS, Huang WP, Hou CC, Chuang HL, Huang CC. Pumpkin (Cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice. Molecules. 2012 Oct 9;17(10):11864-76. [10.3390/molecules171011864 ][PMID]
29.  Michalickova D, Minic R, Kotur-Stevuljevic J, Andjelkovic M, Dikic N, Kostic-Vucicevic M, Slanar O, Djordjevic B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter. The Journal of Strength & Conditioning Research. 2020 Oct 1;34(10):2965-73. [10.1519/JSC.0000000000002780 ][PMID]
30.  Mohazzab M, Matinhomaee H, Hosseini SA, Rahmati Ahmad Abad S. The Effect of Eight Weeks of Increasing Aerobic Training and Pumpkin Seed Supplementation on Oxidative Stress Indices of Lung Tissue in Rats Poisoned with Hydrogen Peroxide. Sport Physiology. 2022 Apr 21;14(53):202-177.
31.  Thirupathi A, Wang M, Lin JK, Fekete G, István B, Baker JS, Gu Y. Effect of different exercise modalities on oxidative stress: a systematic review. BioMed Research International. 2021;2021(1):1947928. [10.1155/2021/1947928 ][PMID]
32.  Heitkamp HC, Wegler S, Brehme U, Heinle H. Effect of an 8-week endurance training program on markers of antioxidant capacity in women. Journal of sports medicine and physical Fitness. 2008 Mar 1;48(1):113. [PMID]
33.  Soares JP, Silva AM, Oliveira MM, Peixoto F, Gaivão I, Mota MP. Effects of combined physical exercise training on DNA damage and repair capacity: role of oxidative stress changes. Age. 2015 Jun;37(3):61. [10.1007/s11357-015-9799-4 ][PMID]
34.  Brown LR, Deng J, Noll DM, Mori N, Clarke ND. Construction and overexpression of a synthetic gene for human DNA methylguanine methyltransferase: renaturation and rapid purification of the protein. Protein expression and purification. 1997 Apr 1;9(3):337-45. [10.1006/prep.1996.0719 ][PMID]
35.  Ogawa Y, Takahashi J, Sakuyama A, Xu L, Miura T, Muroya Y, Ito D, Kohzuki M, Ito O. Exercise training delays renal disorders with decreasing oxidative stress and increasing production of 20-hydroxyeicosatetraenoic acid in Dahl salt-sensitive rats. Journal of hypertension. 2020 Jul 1;38(7):1336-46. [10.1097/HJH.0000000000002409 ][PMID]
36.  Niture SK, Khatri R, Jaiswal AK. Regulation of Nrf2—an update. Free Radical Biology and Medicine. 2014 Jan 8;66:36-44. [10.1016/j.freeradbiomed.2013.02.008 ][PMID]
37.  Jafari M, Motamedi P, Khaledi N. The Effect of Chronic Kidney Disease Induction Upon Affective Factors on Muscle Apoptosis in Rats and the Role of Aerobic Training in Its Modulation. Jundishapur Scientific Medical Journal. 2021 Sep 23;20(4):382-9.

  • Receive Date 24 February 2025
  • Revise Date 22 April 2025
  • Accept Date 27 May 2025