Jundishapur Scientific Medical Journal

Jundishapur Scientific Medical Journal

The Effect of Oral Royal Jelly Supplementation and Exercise Training on Antioxidant Defense-Related Parameters in Healthy Active Girls

Document Type : Original Article

Authors
Saeede Kargaran ardakani 1
Pezhman Motamed, 2
Amani-Shalamzari Sadegh 3
1 PhD Student, Department of Exercise Physiology, Faculty of Sports Sciences, Kharazmi University, Tehran, Iran.
2 Assistant Professor, Department of Exercise Physiology, Faculty of Sports Sciences, Kharazmi University, Tehran, Iran.
3 Associate Professor, Department of Exercise Physiology, Faculty of Sports Sciences, Kharazmi University, Tehran, Iran.
10.32592/jsmj.24.2.141
Abstract
Background and Objectives Royal jelly is a highly nutritious natural product with significant potential as a health-promoting food. The aim of the present study was to determine the effect of six weeks of oral royal jelly supplementation and exercise training on total antioxidant capacity (TAC) and serum malondialdehyde (MDA) levels in healthy active young girls.
Subjects and Methods In this single-blind, semi-experimental study, 40 active women aged 20-25 years with a body mass index of 18-25 kg/m² were randomly divided into four equal groups (n=10 each): 1) Supplement, 2) Exercise+Placebo, 3) Exercise+Supplement, and 4) Placebo. The exercise protocol consisted of six weeks of combined training (high-intensity interval training and resistance training) performed three times per week. The supplement group received 1000 mg royal jelly capsules three times daily. Blood samples were collected after 12 hours of fasting at baseline and 72 hours after the intervention period.
Results A significant increase in TAC and decrease in MDA were observed in the Supplement and Exercise+Supplement groups compared to the Placebo group (p<0.01). Additionally, TAC was significantly higher in the Supplement group compared to the Exercise+Placebo group (p<0.05). No significant differences in MDA were found between the intervention groups (p>0.05).
Conclusion Based on the results, combined exercise training and royal jelly supplementation have synergistic effects in enhancing antioxidant defense and reducing lipid peroxidation in active young women.
Keywords
Oxidative stress
Antioxidant
Exercise training
Free radicals
Royal jelly
Subjects
  • Wahl P, Bloch W, Proschinger S. The molecular signature of high-intensity training in the human body. International journal of sports medicine. 2022 Mar;43(03):195-205. [1055/a-1551-9294 ] [PMID]
  • Ramos JS, Dalleck LC, Tjonna AE, Beetham KS, Coombes JS. The impact of high-intensity interval training versus moderate-intensity continuous training on vascular function: a systematic review and meta-analysis. Sports medicine. 2015 May;45:679-92. [1007/s40279-015-0321-z ] [PMID]
  • Magherini F, Fiaschi T, Marzocchini R, Mannelli M, Gamberi T, Modesti PA, Modesti A. Oxidative stress in exercise training: The involvement of inflammation and peripheral signals. Free radical research. 2019 Dec 2;53(11-12):1155-65. [1080/10715762.2019.1697438 ] [PMID]
  • Wang F, Wang X, Liu Y, Zhang Z. Effects of exercise‐induced ROS on the pathophysiological functions of skeletal muscle. Oxidative medicine and cellular longevity. 2021;2021(1):3846122. [1155/2021/3846122 ] [PMID]
  • Azizbeigi K, Stannard SR, Atashak S, Haghighi MM. Antioxidant enzymes and oxidative stress adaptation to exercise training: Comparison of endurance, resistance, and concurrent training in untrained males. Journal of exercise science & fitness. 2014 Jun 1;12(1):1-6.
  • Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of exercise rehabilitation. 2016 Apr 26;12(2):113. [12965/jer.1632598.299 ] [PMID]
  • Freitas DA, Soares BA, Nonato LF, Fonseca SR, Martins JB, Mendonça VA, Lacerda AC, Massensini AR, Poortamns JR, Meeusen R, Leite HR. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiology & behavior. 2018 Feb 1;184:6-11. [1016/j.physbeh.2017.10.027 ] [PMID]
  • Zwetsloot KA, Nieman DC, Knab A, John CS, Lomiwes DD, Hurst RD, Gillitt ND, Lila MA. Effect of 4 weeks of high‐intensity interval training on exercise performance and markers of inflammation and oxidative stress. The FASEB journal. 2017 Apr;31:839-1.
  • McQuilliam SJ, Clark DR, Erskine RM, Brownlee TE. Free-weight resistance training in youth athletes: a narrative review. Sports Medicine. 2020 Sep;50(9):1567-80. [1007/s40279-020-01307-7 ] [PMID]
  • Schoenfeld B, Fisher J, Grgic J, Haun C, Helms E, Phillips S, Steele J, Vigotsky A. Resistance training recommendations to maximize muscle hypertrophy in an athletic population: Position stand of the IUSCA. International Journal of Strength and Conditioning. 2021 Aug 16;1(1).
  • Sousa AC, Neiva HP, Izquierdo M, Cadore EL, Alves AR, Marinho DA. Concurrent training and detraining: brief review on the effect of exercise intensities. International journal of sports medicine. 2019 Nov;40(12):747-55. [1055/a-0975-9471 ] [PMID]
  • Da Silva MA, Baptista LC, Neves RS, De França E, Loureiro H, Lira FS, Caperuto EC, Veríssimo MT, Martins RA. The effects of concurrent training combining both resistance exercise and high-intensity interval training or moderate-intensity continuous training on metabolic syndrome. Frontiers in physiology. 2020 Jun 11;11:572. [3389/fphys.2020.00572 ] [PMID]
  • Schlegel P. CrossFit® training strategies from the perspective of concurrent training: A systematic review. Journal of sports science & medicine. 2020 Nov 19;19(4):670. [PMID]
  • Ezzati A, Atashak S, Bonab RR. The effect of concurrent training order on oxidative stress biomarkers in sedentary obese women.
  • Ranjbar N, Marandi SM, Namayandeh SM, Mirhosseini J, Ghanbery M. Comparison of Different Time Course of Concurrent Training on Lipid Profile and Total Antioxidant in Cardiac Patients. SSU_Journals. 2022 Dec 15;30(9):5236-47.
  • Radovanovic D, Bratic M, Nurkic M, Cvetkovic T, Ignjatovic A, Aleksandrovic M. Oxidative stress biomarker response to concurrent strength and endurance training. Gen Physiol Biophys. 2009 Jan 1;28(Special Issue):205-11.[PMID]
  • Cordiano R, Di Gioacchino M, Mangifesta R, Panzera C, Gangemi S, Minciullo PL. Malondialdehyde as a potential oxidative stress marker for allergy-oriented diseases: an update. Molecules. 2023 Aug 9;28(16):5979. [3390/molecules28165979 ] [PMID]
  • Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, metabolism and cardiovascular diseases. 2005 Aug 1;15(4):316-28. [1016/j.numecd.2005.05.003] [PMID]
  • Demirci-Çekiç S, Özkan G, Avan AN, Uzunboy S, Çapanoğlu E, Apak R. Biomarkers of oxidative stress and antioxidant defense. Journal of pharmaceutical and biomedical analysis. 2022 Feb 5;209:114477. [1016/j.jpba.2021.114477] [PMID]
  • Halliwell B. Understanding mechanisms of antioxidant action in health and disease. Nature Reviews Molecular Cell Biology. 2024 Jan;25(1):13-33. [1038/s41580-023-00645-4 ] [PMID]
  • Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, Calina D, Sharifi-Rad J. Oxidative stress, free radicals and antioxidants: Potential crosstalk in the pathophysiology of human diseases. Frontiers in chemistry. 2023 May 10;11:1158198. [3389/fchem.2023.1158198] [PMID]
  • Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, Valko M. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic diseases and aging. Archives of toxicology. 2023 Oct;97(10):2499-574. [1007/s00204-023-03562-9] [PMID]
  • Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 2003 Jul 15;189(1-2):41-54. [1016/s0300-483x(03)00151-3 ] [PMID]
  • Lee JS, Hong EK. Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris. International immunopharmacology. 2011 Sep 1;11(9):1226-33. [1016/j.intimp.2011.04.001] [PMID]
  • Ali AM, Kunugi H. Apitherapy for age-related skeletal muscle dysfunction (sarcopenia): A review on the effects of royal jelly, propolis, and bee pollen. Foods. 2020 Sep 25;9(10):1362. [3390/foods9101362] [PMID]
  • Qiu W, Chen X, Tian Y, Wu D, Du M, Wang S. Protection against oxidative stress and anti-aging effect in Drosophila of royal jelly-collagen peptide. Food and Chemical Toxicology. 2020 Jan 1;135:110881. [1016/j.fct.2019.110881 ] [PMID]
  • Ali AM, Kunugi H. The effects of royal jelly acid, 10-hydroxy-trans-2-decenoic acid, on neuroinflammation and oxidative stress in astrocytes stimulated with lipopolysaccharide and hydrogen peroxide. Immuno. 2021 Jul 12;1(3):212-22.
  • Anugrah SM, Kusnanik NW, Wahjuni ES, Ayubi N, Mulyawan R. Effect of royal jelly on performance and inflammatory response to muscle damage: A systematic review. Biointerface Research in Applied Chemistry. 2023;13(5):6-13.
  • Ovchinnikov AN, Paoli A, Seleznev VV, Deryugina AV. Royal jelly plus coenzyme Q10 supplementation improves high-intensity interval exercise performance via changes in plasmatic and salivary biomarkers of oxidative stress and muscle damage in swimmers: a randomized, double-blind, placebo-controlled pilot trial. Journal of the International Society of Sports Nutrition. 2022 Dec 31;19(1):239-57. [1080/15502783.2022.2086015 ] [PMID]
  • Seipp D, Quittmann OJ, Fasold F, Klatt S. Concurrent training in team sports: A systematic review. International Journal of
  • Sports Science & Coaching. 2023 Aug;18(4):1342-64.
  • Collazo N, Carpena M, Nuñez-Estevez B, Otero P, Simal-Gandara J, Prieto MA. Health promoting properties of bee royal jelly: Food of the queens. Nutrients. 2021 Feb 7;13(2):543. [3390/nu13020543 ] [PMID]
  • Khoshpey B, Djazayeri S, Amiri F, Malek M, Hosseini AF, Hosseini S, Shidfar S, Shidfar F. Effect of royal jelly intake on serum glucose, apolipoprotein AI (ApoA-I), apolipoprotein B (ApoB) and ApoB/ApoA-I ratios in patients with type 2 diabetes: a randomized, double-blind clinical trial study. Canadian journal of diabetes. 2016 Aug 1;40(4):324-8. [1016/j.jcjd.2016.01.003 ] [PMID]
  • Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or foe?. Journal of sport and health science. 2020 Sep 1;9(5):415-25. [1016/j.jshs.2020.04.001 ] [PMID]
  • Thirupathi A, Pinho RA. Effects of reactive oxygen species and interplay of antioxidants during physical exercise in skeletal muscles. Journal of Physiology and Biochemistry. 2018 Aug;74:359-67. [1007/s13105-018-0633-1] [PMID]
  • Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of exercise rehabilitation. 2016 Apr 26;12(2):113. [12965/jer.1632598.299] [PMID]
  • Paes L, Lima D, Matsuura C, de Souza MD, Cyrino F, Barbosa C, Ferrão F, Bottino D, Bouskela E, Farinatti P. Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats. PloS one. 2020 Feb 7;15(2):e0218228. [1371/journal.pone.0218228] [PMID]
  • Schneider CD, Barp J, Ribeiro JL, Belló-Klein A, Oliveira AR. Oxidative stress after three different intensities of running. Canadian journal of applied physiology. 2005 Dec 1;30(6):723-34. [1139/h05-151 ] [PMID]
  • Chi X, Liu Z, Wang H, Wang Y, Wei W, Xu B. Royal jelly enhanced the antioxidant activities and modulated the gut microbiota in healthy mice. Journal of food biochemistry. 2021 May;45(5):e13701.

[40] 

 

[41]               

Authors retain the copyright and full publishing rights.

Published by ahvaz jundishapur university of medical science. This article is an open access article licensed under the Creative Commons Attribution-NonCommercial 4.0 (https://creativecommons.org/licenses/by-nc/4.0/).

 
  • Wen D, Xie J, Yuan Y, Shen L, Yang Y, Chen W. The endogenous antioxidant ability of royal jelly in Drosophila is independent of Keap1/Nrf2 by activating oxidoreductase activity. Insect science. 2024 Apr;31(2):503-23.
  • Karadeniz A, Simsek N, Karakus E, Yildirim S, Kara A, Can I, Kisa F, Emre H, Turkeli M. Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin. Oxidative medicine and cellular longevity. 2011;2011(1):981793. [1155/2011/981793 ] [PMID]
  • Anugrah SM, Kusnanik NW, Wahjuni ES, Resmana D, Zubaida I, Aqobah QJ, Nuryadin A, Rahayu A. Evaluating the impact of three dosages of royal jelly supplement on malondialdehyde levels and pain intensity following high-intensity weight training. Fizjoterapia Polska. 2024 Mar 1(2).
  • Poorshamohammad C, Hashemnia M, Nikoosafat Z. Gastroprotective and Antioxidant Effects of Royal Jelly on Ethanol-Induced Gastric Ulcer in Rats. Journal of Mazandaran University of Medical Sciences. 2024;34(231):47-53.

 

  • Receive Date 14 September 2024
  • Revise Date 31 December 2024
  • Accept Date 01 January 2025