Jundishapur Scientific Medical Journal

Jundishapur Scientific Medical Journal

The Effect of Eight Weeks of Resistance Training and Supplementation with Branched-Chain Amino Acids Nanoliposome on the Expression of Nuclear Respiratory Factor 1 and 2 Genes in the Cardiomyocytes of Male Rat Model of Myocardial Infarction

Document Type : Original Article

Authors
Javid Najafi 1
Elham Farhadfar 2
Mohammad Azimnezhad 3
Leila Momeni 2
1 Department of Sport Physiology , CT. C. , Islamic Azad University,Tehran, Iran.
2 Department of Physical Education and Sport Sciences, Dez.C., Islamic Azad University, Dezful, Iran
3 Department of Sport Physiology, CT. C. , Islamic Azad University,Tehran, Iran.
10.32592/jsmj.24.1.80
Abstract
Background and Objectives Restoring and maintaining mitochondrial function due to myocardial infarction is an important therapeutic strategy to protect the heart. The aim of the present study was to determine the effect of eight weeks of resistance training and supplementation with branched amino acid (BCAA) nanoliposome on the expression of nuclear respiratory factor 1 and 2 genes in the cardiomyocytes of male rat model of myocardial infarction.
Subjects and Methods In this experimental study, 32 male Wistar rats were divided into 4 groups of resistance training, supplement, combined (resistance training + supplement) and control after induction of myocardial infarction by intraperitoneal injection of isoproterenol. Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. In the supplement and combined groups, 5 days a week and for 8 weeks, BCAA nanoliposome supplement was received by gavage in the amount of 600 mg per kilogram of body weight. The expression of NRF-1 and NRF-2 genes was measured by Real-time PCR method.
Results The mean expression of NRF-1 and NRF2 genes in cardiomyocytes in exercise and combination groups was significantly higher than in control and supplement groups (P < 0.001). No significant difference was observed between the control and supplement groups (P<0.05).
Conclusion Based on the results of resistance training, by increasing NRF-1 and NRF-2, it improves the function of mitochondria in cardiomyocytes of heart attack model rats.
Keywords
Myocardial infarction
Resistance training
Branched-chain amino acids
Nuclear respiratory factor
Subjects

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  • Receive Date 03 August 2024
  • Revise Date 04 November 2024
  • Accept Date 16 November 2024