Jundishapur Journal of Medical Sciences

Jundishapur Journal of Medical Sciences

The Effect of Eight Weeks of Resistance Training and Supplementation with Branched-Chain Amino Acids Nanoliposome on the Expression of Nuclear Respiratory Factor 1 and 2 Genes in the Cardiomyocytes of Male Rat Model of Myocardial Infarction

Document Type : Original Article

Authors
Javid Najafi 1
Elham Farhadfar 2
Mohammad Azimnezhad 3
Leila Momeni 2
1 Department of Sport Physiology , CT. C. , Islamic Azad University,Tehran, Iran.
2 Department of Physical Education and Sport Sciences, Dez.C., Islamic Azad University, Dezful, Iran
3 Department of Sport Physiology, CT. C. , Islamic Azad University,Tehran, Iran.
10.32592/jsmj.24.1.80
Abstract
Background and Objectives Restoring and maintaining mitochondrial function due to myocardial infarction is an important therapeutic strategy to protect the heart. The aim of the present study was to determine the effect of eight weeks of resistance training and supplementation with branched amino acid (BCAA) nanoliposome on the expression of nuclear respiratory factor 1 and 2 genes in the cardiomyocytes of male rat model of myocardial infarction.
Subjects and Methods In this experimental study, 32 male Wistar rats were divided into 4 groups of resistance training, supplement, combined (resistance training + supplement) and control after induction of myocardial infarction by intraperitoneal injection of isoproterenol. Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. In the supplement and combined groups, 5 days a week and for 8 weeks, BCAA nanoliposome supplement was received by gavage in the amount of 600 mg per kilogram of body weight. The expression of NRF-1 and NRF-2 genes was measured by Real-time PCR method.
Results The mean expression of NRF-1 and NRF2 genes in cardiomyocytes in exercise and combination groups was significantly higher than in control and supplement groups (P < 0.001). No significant difference was observed between the control and supplement groups (P<0.05).
Conclusion Based on the results of resistance training, by increasing NRF-1 and NRF-2, it improves the function of mitochondria in cardiomyocytes of heart attack model rats.
Keywords
Myocardial infarction
Resistance training
Branched-chain amino acids
Nuclear respiratory factor
Subjects

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    • Fathima SN. An update on myocardial infarction. Current Research and Trends in Medical Science and Technology. 2021;1:95.
    • Huang Y, Zhou B. Mitochondrial dysfunction in cardiac diseases and therapeutic strategies. Biomedicines. 2023 May 22;11(5):1500.
    • Guo Y, Guan T, Shafiq K, Yu Q, Jiao X, Na D, Li M, Zhang G, Kong J. Mitochondrial dysfunction in aging. Ageing research reviews. 2023 Jul 1;88:101955. [1016/j.arr.2023.101955 ] [PMID]
    • Scarpulla RC. Nuclear control of respiratory gene expression in mammalian cells. Journal of cellular biochemistry. 2006 Mar 1;97(4):673-83. [1016/j.arr.2023.101955 ] [PMID]
    • Ghalavand A, Shakeryan S, Nikbakht A, Mehdipour AM, Monazamnezhad A, Delaramnasab M. Effects of aerobic training on cardiorespiratory factors in men with type 2 diabetes.
    • Jokar M, Ghalavand A. Improving endothelial function following regular pyramid aerobic training in patients with type 2 diabetes.
    • Paluch AE, Boyer WR, Franklin BA, Laddu D, Lobelo F, Lee DC, McDermott MM, Swift DL, Webel AR, Lane A. Resistance exercise training in individuals with and without cardiovascular disease: 2023 update: a scientific statement from the American Heart Association. Circulation. 2024 Jan 16;149(3):e217-31. [1161/CIR.0000000000001189 ] [PMID]
    • Ko TH, Marquez JC, Kim HK, Jeong SH, Lee S, Youm JB, Song IS, Seo DY, Kim HJ, Won DN, Cho KI. Resistance exercise improves cardiac function and mitochondrial efficiency in diabetic rat hearts. Pflügers Archiv-European Journal of Physiology. 2018 Feb;470:263-75. [1007/s00424-017-2076-x ] [PMID]
    • Taheri Gandmani R, Demirchi A, Mirzaei B. The effect of high and moderate resistance training intensities on the gen expression of AMPK, PGC-1α, TFAM and cytochrome-C of cardiac myocytes in elderly Wistar rats. Iranian Journal of Physiology and Pharmacology. 2022 Mar 10;6:1-9.
    • McGarrah RW, White PJ. Branched-chain amino acids in cardiovascular disease. Nature Reviews Cardiology. 2023 Feb;20(2):77-89. [1038/s41569-022-00760-3 ] [PMID]
    • Ahmed RF, Abd Elbaset M, Farouk H, Shabana ME, Ahmed-Farid OA, Nasr M. Nanoliposomal amino acids counteracting protein malnutrition induced hematopoietic and hepatic complications. Journal of Drug Delivery Science and Technology. 2023 Nov 1;89:105051.
    • Hajighasem A, Ehsanimoghadam S. The effect of swimming training and liposomal BCAA supplementation on miR-133 and miR-206 gene expression in soleus muscle mitochondria of aged male rats. Feyz Medical Sciences Journal. 2024 Mar 10;28(1):10-6.
    • Jghef MM, Boukholda K, Chtourou Y, Fiebich BL, Kebieche M, Soulimani R, Chigr F, Fetoui H. Punicalagin attenuates myocardial oxidative damage, inflammation, and apoptosis in isoproterenol-induced myocardial infarction in rats: Biochemical, immunohistochemical, and in silico molecular docking studies. Chemico-Biological Interactions. 2023 Nov 1;385:110745. [1016/j.cbi.2023.110745 ] [PMID]
    • Teimoori A, Ruzbahani A, Karimimehr Z. The effect of eight weeks of increasing resistance training and BCAA nanoliposome supplementation on MiR-200a, HSP60, soleus muscle mitochondrial gene expression and serum IGF-1 in aged male rats. Metabolism and Exercise. 2023;13(2):101-14.
    • Nikoozadeh A, Po uzesh Jadidi R, Nourazar MA, Asgharpour Arshad M, Bashiri J. Interactive Effect of Aerobic Training and Curcumin Supplementation on Mitochondrial Cardiomyocyte Nrf1 and Nrf2 Gene Expression in Myocardial infraction Male Rat Model. Journal of Applied Health Studies in Sport Physiology. 2021 Sep 23;8(2):44-50.
    • Jia D, Hou L, Lv Y, Xi L, Tian Z. Postinfarction exercise training alleviates cardiac dysfunction and adverse remodeling via mitochondrial biogenesis and SIRT1/PGC‐1α/PI3K/Akt signaling. Journal of cellular physiology. 2019 Dec;234(12):23705-18. [1002/jcp.28939 ] [PMID]
    • Bruning RS, Sturek M. Benefits of exercise training on coronary blood flow in coronary artery disease patients. Progress in cardiovascular diseases. 2015;57(5):443-53. Bruning RS, Sturek M. Benefits of exercise training on coronary blood flow in coronary artery disease patients. Progress in cardiovascular diseases. 2015 Mar 1;57(5):443-53. [1016/j.pcad.2014.10.006 ] [PMID]
    • Steele J, Fisher J, McGuff D, Bruce-Low S, Smith D. Resistance training to momentary muscular failure improves cardiovascular fitness in humans: a review of acute physiological responses and chronic physiological adaptations. Journal of Exercise Physiology Online. 2012;15(3):53-80.
    • Tao L, Bei Y, Lin S, Zhang H, Zhou Y, Jiang J, Chen P, Shen S, Xiao J, Li X. Exercise training protects against acute myocardial infarction via improving myocardial energy metabolism and mitochondrial biogenesis. Cellular Physiology and Biochemistry. 2015 Aug 1;37(1):162-75. [1159/000430342 ] [PMID]
    • Ojuka EO. Role of calcium and AMP kinase in the regulation of mitochondrial biogenesis and GLUT4 levels in muscle. Proceedings of the Nutrition Society. 2004 May;63(2):275-8. [1079/PNS2004339 ] [PMID]
    • Gleyzer N, Vercauteren K, Scarpulla RC. Control of mitochondrial transcription specificity factors (TFB1M and TFB2M) by nuclear respiratory factors (NRF-1 and NRF-2) and PGC-1 family coactivators. Molecular and cellular biology. 2005 Feb 1;25(4):1354-66. [1128/MCB.25.4.1354-1366.2005 ] [PMID]
    • Lou H, Yao J, Zhang Y, Wu X, Sun L, Wang Y, Cong D. Potential effect of acupuncture on mitochondrial biogenesis, energy metabolism and oxidation stress in MCAO rat via PGC-1α/NRF1/TFAM pathway. Journal of Stroke and Cerebrovascular Diseases. 2024 Nov 1;33(11):107636. [1016/j.jstrokecerebrovasdis.2024.107636 ] [PMID]
    • Qi B, Song L, Hu L, Guo D, Ren G, Peng T, Liu M, Fang Y, Li C, Zhang M, Li Y. Cardiac-specific overexpression of Ndufs1 ameliorates cardiac dysfunction after myocardial infarction by alleviating mitochondrial dysfunction and apoptosis. Experimental & molecular medicine. 2022 Jul;54(7):946-60. [1038/s12276-022-00800-5 ] [PMID]
    • Ramachandra CJ, Hernandez-Resendiz S, Crespo-Avilan GE, Lin YH, Hausenloy DJ. Mitochondria in acute myocardial infarction and cardioprotection. EBioMedicine. 2020 Jul 1;57. [1016/j.ebiom.2020.102884 ] [PMID]
    • D'Antona G, Ragni M, Cardile A, Tedesco L, Dossena M, Bruttini F, Caliaro F, Corsetti G, Bottinelli R, Carruba MO, Valerio A. Branched-chain amino acid supplementation promotes survival and supports cardiac and skeletal muscle mitochondrial biogenesis in middle-aged mice. Cell metabolism. 2010 Oct 6;12(4):362-72. [1016/j.cmet.2010.08.016 ] [PMID]
    • Hinkle JS, Rivera CN, Vaughan RA. Branched‐Chain Amino Acids and Mitochondrial Biogenesis: An Overview and Mechanistic Summary. Molecular Nutrition & Food Research. 2022 Oct;66(20):2200109. [1002/mnfr.202200109 ] [PMID]

     

     

  • Receive Date 03 August 2024
  • Revise Date 04 November 2024
  • Accept Date 16 November 2024