Jundishapur Scientific Medical Journal

Jundishapur Scientific Medical Journal

The Effect of Six Weeks’ Melatonin Supplementation and Aerobic Training on Modulating Interleukin-6 gene Expression in Heart Tissue of Male Diabetic Model Rats (Interactive Effect of Aerobic Exercise and Melatonin Supplementation on Interleukin-6 in Diabetic Cardiomyopathy)

Document Type : Original Article

Authors
Sara Salavati 1
Marina Shariati 2
1 MSc in Sport Nutrition, Department of Physical Education and Sports Sciences, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
2 Department of Physical Education and Sports Sciences, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
10.32592/jsmj.23.6.560
Abstract
Background and Objectives The relationship between heart diseases and oxidative stress has been determined. The aim of this research was to determine the effect of aerobic training along with melatonin on the expression of interleukin-6 (IL-6) gene in the heart tissue of diabetic male rats.
Subjects and Methods In this experimental research, 64 eight-week-old male Wistar rats (weight range: 204 ± 11.3 grams) were randomly were placed into eight groups: diabetes control, diabetes+melatonin, diabetes+exercise,diabetes+melatonin+exercise.Healthy+melatonin,healthy+exercise,healthy+melatonin+exercise and healthy controls. Diabetes was induced by injecting streptozotocin 50 mg/kg of rat weight. Then, the exercise groups performed six weeks of continuous aerobic training on the treadmill. Also, melatonin was injected at a dose of 10 mg/kg of weight daily for 6 weeks. The level of IL-6 gene expression in heart tissue was measured by real time technique. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis and the significance level (P ≥ 0.05) was considered.
Results The results showed that the induction of diabetes caused a significant increase in IL-6 in the diabetic groups compared to the healthy groups (P<0.05). Also, there was a significant decrease in IL-6 gene expression in the diabetes+melatonin+exercise group compared to the control diabetes group (P<0.05).
Conclusion Based on the results of aerobic exercise along with melatonin supplementation can reduce inflammation in the heart tissue of diabetic rats by reducing IL-6 and thus prevent diabetic cardiomyopathy.
Keywords
Diabetes
Cardiomyopathy
Aerobic training
Melatonin
Interleukin-6
Subjects

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Jundishapur Scientific Medical Journal
Volume 23, Issue 6 - Serial Number 153
January and February 2025
Pages 560-572

  • Receive Date 12 July 2024
  • Revise Date 08 September 2024
  • Accept Date 08 September 2024