Jundishapur Journal of Medical Sciences

Jundishapur Journal of Medical Sciences

The effect of detraining and retraining on the gene expression of ATF4 and P53 in rat plantaris muscle after hindlimb suspension

Document Type : Original Article

Authors
Abdereza Abdereza Kazemi 1
Fatemeh Zain al-Dini Raviz 2
1 Associate Prof, Department of Sports Science, Faculty of Literature and Humanities, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
2 MSc in Exercise Physiology, Department of Sports Science, Faculty of Literature and Humanities, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
10.32592/JSMJ.23.4.273
Abstract
Background and objectives: Resistance training increases muscle mass and inhibits atrophy by changing the muscle mass and regulating the expression of muscle proteins. The purpose of this study was to investigate the effect of a detraining and retraining period on gene expression of ATF4 and P53 in the plantaris muscle of rats after hindlimb suspension.
Subjects and Methods: 32 male rats aged 4 to 6 months were randomly divided into four groups (N=8): 1- control (G1), 2- training-suspension-retraining (G2), 3- detraining-suspension-retraining (G3) and 4- training-suspension-detraining (G4). The duration of resistance training was four weeks and the duration of the suspension protocol was two weeks. At the end of the study, the animals were anesthetized using chloroform and dissected and their plantaris muscle was separated. The measurement of gene expression was done by RT-PCR. Anova and Tukey's test were used to determine the difference between variables.
Results: A significant difference was observed between the research groups in ATF4 and p53 gene expression (F=16.57) (p=0.0001) and (F=55.07) (p=0.0001). In addition, there was a significant decrease in ATF4 and p53 gene expression in G2, G3 and G4 compared to G1. Also, ATF4 gene expression was significantly decreased in G2 compared to G3 (p<0.05).
Conclusion: Overall, the findings of the present study showed that resistance training is a very effective tool in preventing and controlling atrophy caused by immobility in animal model.
Keywords
Resistance Training
P53
ATF4
Hindlimb suspension

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Jundishapur Journal of Medical Sciences
Volume 23, Issue 4 - Serial Number 151
September and October 2024
Pages 273-285

  • Receive Date 11 March 2024
  • Revise Date 16 April 2024
  • Accept Date 13 May 2024