Jundishapur Scientific Medical Journal

Jundishapur Scientific Medical Journal

Regulation of increased expression of genes involved in mitochondrial dynamics of testicular tissue (PGC1-α and OPA1) in azoospermia model rats in interventions based on laser and physical activity

Document Type : Original Article

Authors
1 PhD student, Department of Physical Education & Sports Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
2 Assistant professor, Department of Physical Education & Sports Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
3 Associated professor, Department of Physical Education & Sports Sciences, Sari Branch, Islamic Azad University, Sari, Iran.
4 Department of Physical Education & Sports Sciences,, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, . Iran
10.32592/JSMJ.23.1.89
Abstract
Background: The underlying molecular mechanisms and effects of physical exercise on azoospermia are not well understood.The purpose of this research is to investigate the effect of cell therapy, laser and activity on the expression of genes involved in mitochondrial biogenesis in azoospermia model rats.

Methods: 40 8-week-old rats were randomly selected, and then the azoospermia model was induced with the drug busulfan with a dose of 40 mg, and after one month, each group of rats was divided into groups. One month after the creation of a one-time model, stem cells were transplanted in the vas deferens in the amount of one million cells for each mouse. After a week of cell transplantation, a laser with a wavelength of 632.8 nm And the power of 10 milliwatts and energy of 3 joules was applied three times during the entire study period with an interval of one week and after the healing of the wound of the cell transplant area on the abdomen, daily for 30 minutes, 5 days in They performed swimming exercises every week for 8 weeks.

Results: Induction of azoospermia model decreased the expression of PGC1α gene and increased the expression of OPA1 gene, which the implementation of each of the interventional methods of laser, exercise, laser-activity and cell-activity reversed these changes.

Conclusion: Therefore, it can be said that in azoospermia model rats, the simultaneous use of interventional methods of activity and laser therapy has the best effectiveness.
Keywords
Subjects

[1] He H, Yu F, Shen W, Chen K, Zhang L, Lou S, Zhang Q, Chen S,
Yuan X, Jia X, Zhou Y. The novel key genes of non-obstructive
azoospermia affect spermatogenesis: transcriptomic analysis
based on RNA-Seq and scRNA-Seq data. Frontiers in Genetics.
2021 Feb 26;12:608629. [10.3389/fgene.2021.608629] [PMID]
[2] Kohn TP, Pastuszak AW. Non-obstructive azoospermia and
shortened leukocyte telomere length: further evidence linking
poor health and infertility. Fertility and Sterility. 2018 Sep
1;110(4):629-30. [10.1016/j.fertnstert.2018.06.013] [PMID]
[3] Abu Shelbayeh O, Arroum T, Morris S, Busch KB. PGC-1α is a
master regulator of mitochondrial lifecycle and ROS stress
response. Antioxidants. 2023 May 10;12(5):1075. [10.3390/
antiox12051075] [PMID].
[4] Halling JF, Pilegaard H. PGC-1α-mediated regulation of
mitochondrial function and physiological implications. Applied
Physiology, Nutrition, and Metabolism. 2020;45(9):927-36.
[10.1139/apnm-2020-0005] [PMID]
[5] Rius-Pérez S, Torres-Cuevas I, Millán I, Ortega ÁL, Pérez S. PGC-
1α, inflammation, and oxidative stress: an integrative view in
metabolism. Oxidative medicine and cellular longevity. 2020
Oct;2020. [10.1155/2020/1452696] [PMID]
[6] Khosrobakhsh F, Moloudi MR, Shoja K, Mohammadi S. Effect
of alpha-lipoic acid on pancreatic optic atrophy 1 (OPA1) gene
expression in male rat model of obstructive cholestasis and
cirrhosis. Scientific Journal of Kurdistan University of Medical
Sciences. 2019 Dec 10;24(5):120-34.
[7] Delettre C, Griffoin JM, Kaplan J, Dollfus H, Lorenz B, Faivre L,
Lenaers G, Belenguer P, Hamel CP. Mutation spectrum and
splicing variants in the OPA1 gene. Human genetics. 2001
Dec;109:584-91. [10.1007/s00439-001-0633-y] [PMID]
[8] Nikbin S, Derakhshideh A, Karimi Jafari S, Mirzahamedani A,
Moslehi A, Ourzamani S, Barati E, Amini F, Zolfaghari FS,
Azarbayjani MA. Investigating the protective effect of aerobic
exercise on oxidative stress and histological damages of testicular
tissue associated with chlorpyrifos in male rats. Andrologia. 2020
Mar;52(2):e13468. [10.1111/and.13468] [PMID]
[9] Dutra Gonçalves G, Antunes Vieira N, Rodrigues Vieira H, Dias
Valério A, Elóisa Munhoz de Lion Siervo G, Fernanda Felipe
Pinheiro P, Eduardo Martinez F, Alessandra Guarnier F,
Rampazzo Teixeira G, Scantamburlo Alves Fernandes G. R ole
of resistance physical exercise in preventing testicular damage
caused by chronic ethanol consumption in UChB rats.
Microscopy Research and Technique. 2017 Apr;80(4):378-86.
[10.1002/jemt.22806] [PMID]
[10] Khosravi Sadr M, Nasiri E, Khalili M. The effect of resistance
training on testicular function and spermatogenesis process
and sperm parameters of adult male Wistar rats. Daneshvar
Medicine. 2021 Jan 3;28(5):11-22.
[11] Thompson M, Mei SH, Wolfe D, Champagne J, Fergusson D,
Stewart DJ, Sullivan KJ, Doxtator E, Lalu M, English SW, Granton
J. Cell therapy with intravascular administration of mesenchymal
stromal cells continues to appear safe: an updated systematic
review and meta-analysis. EClinicalMedicine. 2020 Feb 1;19.
[10.1016/j.eclinm.2019.100249] [PMID]
[12] Kim HJ, Park JS. Usage of human mesenchymal stem cells in
cell-based therapy: advantages and disadvantages. Development
& reproduction. 2017 Mar;21(1):1. [10.12717/DR.2017.21.1.
001] [PMID]
[13] Zhankina R, Baghban N, Askarov M, Saipiyeva D, Ibragimov A,
Kadirova B, Khoradmehr A, Nabipour I, Shirazi R,
Zhanbyrbekuly U, Tamadon A. Mesenchymal stromal/stem
cells and their exosomes for restoration of spermatogenesis in
non-obstructive azoospermia: a systemic review. Stem Cell
Research & Therapy. 2021 Dec;12:1-2. [10.1186/s13287-021-
02295-9] [PMID]
[14] Hasan P, SA R, Purnomo S, Kainama H. The possible application
of low reactive-level laser therapy (LLLT) in the treatment of
male infertility: a preliminary report. Laser therapy. 2004;
14(0_Pilot_Issue_2):0_65-6.
[15] Ziaeipour S, Norouzian M, Abbaszadeh HA, Aliaghaei A,
Nazarian H, Karamian A, Tabeie F, Naserzadeh P, Abdi S,
Abdollahifar MA, Paktinat S. Photobiomodulation therapy
reverses spermatogenesis arrest in hyperthermia-induced
azoospermia mouse model. Lasers in Medical Science. 2023
Apr 27;38(1):114. [10.1007/s10103-023-03780-8] [PMID]
[16] Jafari M, GHALAVAND A, RAJABI H, KHALEDI N, MOTAMEDI P.
A review of the effect of exercise training on neuromuscular
junction in throughout life: A logical analysis of animal
experimental studies.
[17] Ghalavand A, Ghobadi MR. Effect of Exercise and Insulin
Signaling on Glucose Transporter Type 4 in Skeletal Muscles: A
narrative review. Journal of Shahid Sadoughi University of
Medical Sciences. 2023 Mar 27.
[18] Roberts FL, Markby GR. New insights into molecular
mechanisms mediating adaptation to exercise; A review
focusing on mitochondrial biogenesis, mitochondrial function,
mitophagy and autophagy. Cells. 2021 Oct 2;10(10):2639.
[10.3390/cells10102639] [PMID]
[19] Sorriento D, Di Vaia E, Iaccarino G. Physical exercise: a novel
tool to protect mitochondrial health. Frontiers in physiology.
2021 Apr 27;12:660068. [10.3389/fphys.2021.660068] [PMID]
[20] Zohrabi Karani L, Farzanegi P, Azarbayjani MA. The Effect of 8-
Weeks of Low-Intensity Swimming Training on Promyelocytic
Leukemia Zinc Finger Protein and Spermatid Transition Nuclear
Protein Gene Expression in Azoospermic Rats Model. Internal
Medicine Today. 2020 Sep 10;26(4):332-47.
[21] Koochaki S, Abbaszadeh H, FARZANEGI P. Evaluation of SIRT1
and PGC-1α, gene expression in testicular tissue of
azoospermic rats treated with busulfan after a period of
swimming training.
[22] Gao X, Feng B, Du C, Hou C, Jin S, Tang D, Zhu J.
Characterization, expression dynamics, and potential function
of OPA1 for regulation of mitochondrial morphology during
spermiogenesis in Phascolosoma esculenta. Journal of
Oceanology and Limnology. 2024 Jan 11:1-4.
[23] Yang W, Zhang G, Jiang F, Zeng Y, Zou P, An H, Chen Q, Ling X,
Han F, Liu W, Yang H. BPDE and B [a] P induce mitochondrial
compromise by ROS-mediated suppression of the
SIRT1/TERT/PGC-1α pathway in spermatogenic cells both in
vitro and in vivo. Toxicology and Applied Pharmacology. 2019
Aug 1;376:17-37.
102
Shapouri A, et al. Interactive effect of exercise training and laser therapy in azoospermia. JSMJ. 2024; 23(1):89-102
Jundishapur
Scientific Medical Journal
March & April 2024. Vol 23. No 1
[24] Torma F, Koltai E, Nagy E, Ziaaldini MM, Posa A, Koch LG,
Britton SL, Boldogh I, Radak Z. Exercise increases markers of
spermatogenesis in rats selectively bred for low running
capacity. PloS one. 2014 Dec 10;9(12):e114075. [10.1371/
journal.pone.0114075] [PMID]
[25] Samadian Z, Tofighi A, Razi M, Tolouei Azar J, Ghaderi Pakdel
F. Moderate‐intensity exercise training ameliorates the
diabetes‐suppressed spermatogenesis and improves sperm
parameters: Insole and simultaneous with insulin. Andrologia.
2019 Dec;51(11):e13457. [10.1111/and.13457] [PMID]
[26] Jokar M, Ghalavand A. Improving endothelial function
following regular pyramid aerobic training in patients with
type 2 diabetes. Razi Journal of Medical Sciences. 2021 Sep
10;28(6):60-9.
[27] Mruk DD, Sarkar O, Mathur PP. Nitric oxide-cGMP signaling: its
role in cell junction dynamics during spermatogenesis.
Immunology, Endocrine & Metabolic Agents in Medicinal
Chemistry (Formerly Current Medicinal Chemistry-Immunology,
Endocrine and Metabolic Agents). 2008 Mar 1;8(1):28-35.
[28] MacVicar T, Langer T. OPA1 processing in cell death and
disease–the long and short of it. Journal of cell science. 2016
Jun 15;129(12):2297-306. [10.1242/jcs.159186] [PMID]
[29] Von der Malsburg A, Sapp GM, Zuccaro KE, von Appen A, Moss
III FR, Kalia R, Bennett JA, Abriata LA, Dal Peraro M, van der
Laan M, Frost A. Structural mechanism of mitochondrial
membrane remodelling by human OPA1. Nature. 2023 Aug
31;620(7976):1101-8. [10.1038/s41586-023-06441-6] [PMID]
[30] Frezza C, Cipolat S, De Brito OM, Micaroni M, Beznoussenko
GV, Rudka T, Bartoli D, Polishuck RS, Danial NN, De Strooper B,
Scorrano L. OPA1 controls apoptotic cristae remodeling
independently from mitochondrial fusion. Cell. 2006 Jul
14;126(1):177-89. [10.1016/j.cell.2006.06.025] [PMID]
[31] Singh SK, Chakravarty S. Antispermatogenic and antifertility
effects of 20, 25-diazacholesterol dihydrochloride in mice.
Reproductive Toxicology. 2003 Jan 1;17(1):37-44. [10.1016/
s0890-6238(02)00075-8] [PMID]
[32] Deihimi M, Azornia M, Takzare N. Effect of red and infrared
spectrum low level of laser rays on Rat Seminiferous tubules.
Journal of Gorgan University of Medical Sciences. 2010 Oct
10;12(3):10-7.
[33] Mozafar A, Mehranani D, Vahdati A, Hosseini SE, Forouzanfar M.
The Effect of Bone Marrow Mesenchymal Stem Cell Culture in the
Treatment of Azoospermic Infertility induced by Busulfan Balb/C
mice. Armaghane Danesh. 2017 Aug 10;22(3):295-310.
[34] Karimaghai N, Tamadon A, Rahmanifar F, Mehrabani D,
Jahromi AR, Zare S, Khodabandeh Z, Jahromi IR, Koohi-
Hoseinabadi O, Dianatpour M. Spermatogenesis after
transplantation of adipose tissue-derived mesenchymal stem
cells in busulfan-induced azoospermic hamster. Iranian journal
of basic medical sciences. 2018 Jul;21(7):660. [10.22038/
IJBMS.2018.29040.7010] [PMID]
[35] Kamardi MT, Pourgholaminejad A, Eslaminejad MB, Sotoodeh
nejadnematalahi F. Mesenchymal stem cells and their
application in autoimmune disease treatment. Tehran
University Medical Journal. 2014 Sep 1;72(6).
[36] Hoang DM, Pham PT, Bach TQ, Ngo AT, Nguyen QT, Phan TT,
Nguyen GH, Le PT, Hoang VT, Forsyth NR, Heke M. Stem cellbased
therapy for human diseases. Signal transduction and
Volume 23, Issue 1 - Serial Number 148
March and April 2024
Pages 89-102

  • Receive Date 17 September 2023
  • Revise Date 01 February 2024
  • Accept Date 06 February 2024