Molecular separation and cloning of the ATP-binding Cassette (ABC) from Trichophyton Rubru

Document Type : Original Article

Authors

1 1- Department of Microbiology, North Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Science and research Branch, Islamic Azad University, Tehran, Iran

3 Department of Biology, North Branch, Islamic Azad University, Tehran. Iran

Abstract

Background and Objectives: The ABC family of transporters, in addition to their role in cell division and storage of cell volume in fungi, they are reported to play a role in natural metabolism and drug resistance. The purpose of this study is to investigate the molecular separation and cloning of the ATP-binding Cassette (ABC) from Trichophyton rubrum.
Methods: Sixty samples of dermatophytosis were isolated from the patients. Isolates were identified by chemical and molecular tests. Afterward, PCR was performed using specific primers for the ABC gene. PCR product was cloned in the PTG19 vector. Real-Time PCR was performed to evaluate the expression level of ABC gene.
Results: A total of 12 isolates of Trichophyton Rubrum were identified. Six isolates were positive for ABC gene. The expression of the ABC gene cloned in E. coli XL1blue was confirmed by Real time PCR.
Conclusion: Expression of efflux pumps considered a way of resistance to antibiotics in microorganisms. In this regard, the researchers have developed the efflux pump inhibitor compounds and used them in combination with antibiotics to combat infections. However, because of the toxicity of most of these compounds in humans, their extensive use is prohibited. Therefore, finding new inhibitory compounds with little or no toxicity is challenging.

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 19, Issue 4 - Serial Number 127
September and October 2020
Pages 347-355

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History

  • Receive Date: 04 January 2020
  • Revise Date: 20 July 2020
  • Accept Date: 17 August 2020

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