Document Type : Original Article
Authors
1
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4
Department of Infectious Diseases, Razi Teaching Hospital, Ahvaz Jundishapur University of Medical Sciences Ahvaz, Iran.
10.22118/jsmj.2024.434673.3365
Abstract
Abstract
Introduction: Pyrazinamide is one of the effective drugs for the treatment of tuberculosis. The use of this drug in the treatment regimen shortens the duration of tuberculosis treatment. The presence of mutation in pncA gene causes loss of pyrazinamidase activity, which is the most important resistance mechanism in Mycobacterium tuberculosis isolates. Therefore, the purpose of this study is to investigate the phenotypic and genotypic resistance to pyrazinamide antibiotic in multidrug-resistant (MDR) Mycobacterium tuberculosis isolates in Khuzestan province in the period of 1395-1401.
Materials and methods: This study was conducted on 40 isolates of Mycobacterium tuberculosis in Ahvaz TB Regional Laboratory. Determining the drug sensitivity of the isolates was done by the relative method in the antibiotic pyrazinamide in the isolates. Then, the frequency of pncA gene mutations related to pyrazinamide resistance in MDR isolates was determined using sequencing.
Findings: In the results of the drug sensitivity test to rifampin and isoniazid antibiotics, there are 25 isolates, of which 16 strains are resistant to two drugs, isoniazid and rifampin (MDR-TB) and 9 single-drug resistant isolates (8 isolates resistant to rifampin and 1 isolate resistant to Isoniazid) was obtained. Of the 17 samples that had the pncA gene and were resistant to pyrazinamide, 13 isolates had mutations in the pncA gene and 4 isolates did not have any mutations. The most common mutation was a non-synonymous mutation in which the amino acid Val has changed to Phe.
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