Regulation of increased expression of genes involved in mitochondrial dynamics of testicular tissue (PGC1-α and OPA1) in azoospermia model rats in interventions based on laser and physical activity

Document Type : Original Article

Authors

1 Department of Physical Education & Sports Sciences,, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, . Iran

2 Department of Physical Education & Sports Sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul , Iran

3 Department of Physical Education & Sports Sciences, Sari Branch, Islamic Azad University, Sari, Iran

Abstract

Background: The underlying molecular mechanisms and effects of physical exercise on azoospermia are not well understood.The purpose of this research is to investigate the effect of cell therapy, laser and activity on the expression of genes involved in mitochondrial biogenesis in azoospermia model rats.

Methods: 40 8-week-old rats were randomly selected, and then the azoospermia model was induced with the drug busulfan with a dose of 40 mg, and after one month, each group of rats was divided into groups. One month after the creation of a one-time model, stem cells were transplanted in the vas deferens in the amount of one million cells for each mouse. After a week of cell transplantation, a laser with a wavelength of 632.8 nm And the power of 10 milliwatts and energy of 3 joules was applied three times during the entire study period with an interval of one week and after the healing of the wound of the cell transplant area on the abdomen, daily for 30 minutes, 5 days in They performed swimming exercises every week for 8 weeks.

Results: Induction of azoospermia model decreased the expression of PGC1α gene and increased the expression of OPA1 gene, which the implementation of each of the interventional methods of laser, exercise, laser-activity and cell-activity reversed these changes.

Conclusion: Therefore, it can be said that in azoospermia model rats, the simultaneous use of interventional methods of activity and laser therapy has the best effectiveness.

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History

  • Receive Date: 17 September 2023
  • Revise Date: 01 February 2024
  • Accept Date: 06 February 2024

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