Determination of pathotypes and antibiotic resistance patterns of diarrheagenic Escherichia coli strains isolated from children with diarrhea in Khorram Abaad; Lorestan; Iran

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor of Biology.Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 Associate Professor of Biology.Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

4 Assistant Professor of Pathobiology.Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Iran.

Abstract

Background and purpose
Escherichia coli pathogenic strains are considered as the main cause of intestinal and extra-intestinal diseases. This study is aimed to identify and evaluate the dominant pathotypes of diarrheagenic E. coli isolates.
Materials and method
In this current study, 578 diarrhea samples of the children less than 10 years old were collected in Khoram Abbad city. After culture and isolation, the pathogenic groups of diarrheagenic E. coli (DEC) isolates including four pathotypes of Enterotoxigenic E. coli, Enteropathogenic E. coli, Enteroinvasive E. coli, and Shiga toxigenic E. coli were investigated by PCR. Disk diffusion method (Kirby Bauer) was used for detection of antimicrobial sensitivity of the isolates.
Findings
Out of 578 stool samples collected from the children with diarrhea, 81 isolates were determined as DEC. The frequencies of the isolates were respectively reported as the following: enterotoxigenic E. coli (15.1%), enteropathogenic E. coli (14%), enteroinvasive E. coli (5%), and shiga toxigenic E. coli (9.7%). The highest antibiotic resistance levels were respectively reported to: amoxicilin (78%), tetracycline (74.1%), co-trimoxazole (64.2%), nalidixic acid (56.8%), streptomycin (46.9%), minocycline (38.3%), and ciprofloxacin (16.1%). Multiple drug resistance was found in 30.9% of the isolates.
Conclusion
Enterotoxigenic E. coli is one of the most common diarrheagenic agents affecting the children less than 10 years old in this region. So, such diseases can be prevented by controlling water and food hygiene especially in warm seasons.

Keywords

References

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Jundishapur Scientific Medical Journal
Volume 20, Issue 1 - Serial Number 130
March and April 2022
Pages 29-43

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History

  • Receive Date: 11 July 2020
  • Revise Date: 23 October 2020
  • Accept Date: 22 February 2021

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