The Comparison of Effect of Chlorhexidine and Proanthocyanidin and Grape Seed Extract on Composite-Dentin Micro Tensile Bond Strength: an in Vitro Experimental Study

Document Type : Original Article

Authors

1 Assistant Professor of Odontología Operativa.-Department of Odontología Operativa, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Assistant Professor of Odontología Operativa.Department of Odontología Operativa, Faculty of Dentistry, University of Medical Sciences Birjand, Birjand, Iran.

3 Resident of Odontología Operativa.Department of Odontología Operativa, Faculty of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Assistant Professor of Farmacognosia.Department of Farmacognosia, Faculty of Farmaci, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background and Objective: Achieving a strong bond between composite resin and dentin remains a challenge in restorative dentistry. The aim of this study was to evaluate the effect of a 2% chlorhexidine (as a matrix metalloproteinase inhibitor), grape seed extract and proanthocyanidin (as a cross-linking agents to further stabilize collagen fibrils) on the dentin bond strength.
Subjects and Methods: The occlusal enamel was removed from 35 human sound premolars and dentin surfaces etched with phosphoric acid and washed with water. Teeth were allocated into seven groups: chlorhexidine, grape seed extract, proanthocyanidin, chlorhexidine plus grape seed extract, chlorhexidine plus proanthocyanidin, ethanol (the solvent of grape seed extract), and left unexposed to any solution (control). Then, a resin composite restoration was made on the dentin surface after using etch-and-rinse adhesive.
The micro tensile bond strength (µTBS) of the specimens was analyzed after three month of storage in distilled water and 1500 thermocycles.
Results: Multivariate analysis of variance and t-test showed that control group had a higher bond strength and chlorhexidine plus grape seed extract group had a lowest bond strength than another groups. According to variance analysis chlorhexidine and grape seed extract and proanthocyanidin had comprising effect on dentin bond strength.
Conclusion: Use of chlorhexidin, grape seed extract and proanthocyanidin did not increase the composite-dentine micro tensile bond strength.
 

References

1-Monticelli F, Toledano M, Silva AS, Osorio E, Osorio R. Sealing effectiveness of etch-and-rinse vs self-etching adhesives after water aging: influence of acid etching and NaOCl dentin pretreatment. J Adhes Dent. 2008;10(3):183–188.
2-De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G. Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res. 2003; 82: 136-140. 
3-Hashimoto M, Fujita S, Endo K, Ohno H. In vitro degradation of resin-dentin bonds with one-bottle self-etching adhesives. Eur J Oral Sci. 2009; 117: 611-617.  
4-van Dijken JW, Sunnegardh-Gronberg K, Lindberg A. Clinical longterm retention of etch-and-rinse and self-etch adhesive systems in non-carious cervical lesions. A 13 years evaluation. Dent Mater. 2007;23(9):1101–1107.
5-Tay FR, Pashley DH, Suh BI, Carvalho RM, Itthagarun A. Single-step adhesives are permeable membranes. J Dent. 2002;30(7–8):371–382.
6-Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin-dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials. 2003;24(21):3795–3803.
7-Malacarne J, Carvalho RM, de Goes MF, Svizero N, Pashley DH, Tay FR, Yiu CK, Carrilho MR. Water sorption/solubility of dental adhesive resins. Dent Mater. 2006;22(10):973–980.
8-Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83(3):216–221.
9-Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res. 2000;79(6):1385–1391.
10-Marshall GW Jr, Marshal SJ, Kinney JH, Balooch M. The dentin substrate: structure and properties related to bonding [review]. J Dent. 1997, 25:441–458. 
11-Yamauchi M, Shiiba M. Lysine hydroxylation and crosslinking of collagen. Methods Mol Biol. 2002, 194:277–290.  
12-William C. Treeseandeevanspharmacoegnosy. London: crc press.2008,  Chapter 8, page233-235.
13-Castellan  CS, Pereira  PN, Viana G, Chen SN, Pauli GF, Bedran-Russo AK. Solubility study of phytochemical cross-linking agents on dentin stiffness. J Dent. 2010;38(5):6.
14-Han B, Jaurequi  J,  Tang  BW, Nimni  ME. Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices. J Biomed Mater Res A. 2003;65(1):118–124.
15-Bedran-Russo AK,  Pashley DH,  Agee  K, Drummond JL, Miescke KJ. Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B ApplBiomater. 2008;86B(2):330–334.
16-Pashley  DH, Tay  FR, Yiu  C, Hashimoto  M, Breschi  L, Carvalho RM & Ito S. Collagen degradation by host-derived enzymes during aging Journal of Dental Research.2004, 83(3) 216-221.
17-Stanislawczuk  R, Amaral  RC,  Zander-Grande  C, Gagler  D, Reis A, &Loguercio AD . Chlorhexidine-containing acid conditioner preserves the longevity of resindentin bonds Operative Dentistry.2009, 34(4) 481-490.
18-Kim  J, Gu  L, Breschi  L, Tja¨derhane  L, Choi  KK, Pashley  DH &TayFR.Implication of ethanol wetbonding in hybrid layer remineralization Journal of Dental Research.2010, 89(6) 575-580.
19-Simo˜es, Basting RT, Amaral CP, Franc FGM. Influence of Chlorhexidine and/orEthanol Treatment on Bond Strength of an Etch-and- rinse Adhesive to Dentin: An In Vitro and In Situ Study. _Operative Dentistry, 2014, 39-1, 64-71
20-Carrilho MR,  Carvalho  RM,  De Goes  MF, Di Hipólito V, Geraldeli S, TayFR, et al. Chlorhexidine preserves dentin bond in vitro. J DentRes. 2007, 86:90-94.
21-Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De StefanoDorigo E. Dental adhesion review: aging and stability of thebonded interface. Dent Mater. 2008, 24:90-101.
22-Gendron  R,  Grenier  D,  Sorsa  T,  Mayrand  D. Inhibition of the activitiesof matrix metalloproteinases 2, 8, and 9 by chlorhexidine. ClinDiagn Lab Immunol.1999, 6:437-439.
23-Breschi L, Cammelli F, Visintini A, and et al: Influence of chlorhexidine concentration on the durability of etch-and-riinse dentin bonds: a 12-month in vitro study. J Dent Child. 2009 ,76(1):20-7
24-MontagnerA.F, Sarkis-OnofreR,  Pereira-Cenci T, Cenci M.S. Inhibitors on Dentin Stability: A Systematic Review and Meta-analysis. J DENT RES. 2014 93: 733.
25-Collares  FM, Rodrigues SB, Leitune VC, Celeste RK, Borba de Araújo F,Samuel SM. Chlorhexidine application in adhesive procedures:a meta-regression analysis. J Adhes Dent. 2013, 15:11-18.
26-De Munck J, Mine A, Poitevin A, Van Ende A, Cardoso MV, Van LanduytKL, et al. Meta-analytical review of parameters involved indentin bonding. J Dent Res.2012, 91:351-357.
27-Tezvergil-Mutluay A, Agee KA, Hoshika T, Carrilho M, Breschi L, Tjäderhane L, et al. The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices. Dent Mater. 2010, 26:1059-1067.
28-Carina S, Castellana B,  Bedran-Russob K,  Karolb S and PatríciaNóbrega Rodrigues Pereirac. Long-term stability of dentin matrix following treatment with v arious natural collagen cross-linkers. J MechBehav Biomed Mater. 2011 ; 4(7): 1343–1350. 
29-Castellan C, Pereira P, Grande R, and Bedran-Russo A. Mechanical characterization of proanthocyanidin-dentin matrix interaction. Dental Materials Journal. 2010, 26(10): 968–973.
30-Carina S,  CastellanA,  Bedran R,  Alberto A and Patricia N. R.  Effect of Dentin Biomodification Using Naturally Derived Collagen Cross-Linkers: One-Year Bond Strength Study. International Journal of Dentistry Volume 2013,  918010, 6 pages.
31-Nandakumar V, Singh T and Katiyar SK. Multi-targeted prevention and therapy of cancer by proanthocyanidins.  Cancer Letters Journal. 2008, 269( 2):378–387.
32-Matchett M,  MacKinnon SL,  Sweeney MI,  Gottschall-Pass KT, and  Hurta PA. Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells. Biochemistry and Cell Biology Journal. 2005, 83(5): 637–643.
33-Busenlehner LS  and  Armstrong RN. Insights into enzyme structure and dynamics elucidated by amide H/D exchange mass spectrometry. Archives of Biochemistry and Biophysics. 2005, 433(1): 34–46.
34-Weadock K, Olson RM and Silver FH. Evaluation of collagen crosslinking techniques. Biomaterials Medical Devices and Artificial Organs. 1983, 11(4): 293–318.
35-Al-Ammar A,  Drummond J and  Bedran-Russo AK. The use of collagen cross-linking agents to enhance dentin bond strength. Journal of Biomedical Materials Research. 2009, 91(1): 419–424.
Jundishapur Scientific Medical Journal
Volume 15, Issue 3 - Serial Number 102
July and August 2017
Pages 283-292

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History

  • Receive Date: 24 January 2016
  • Revise Date: 17 May 2016
  • Accept Date: 23 May 2016

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