The frequency of Herpes simplex virus type 2 in vaginal and cervix samples in women of Ahvaz city.

Document Type : Original Article

Authors

1 department of biology faculty of science shahid chamran university of Ahvaz, Ahvaz, Iran

2 Department of Biology, Shahid Chamran University of Ahvaz,Ahvaz, Iran

3 Department of Biology, Faculty ofScience, Shahid ChamranUniversity ofAhvaz,Ahvaz,Iran

Abstract

Genital Herpes is one of the most common sexually transmitted disease in the world especially in women and has high prevalence in developed and developing countries. It has third Couse of sexually transmitted diseases after Gonoccocal and Chlamydia infections in the world. HSV-2 is the main couse of genital herpes . The aim of this study was assessment of the prevalence of HSV-2 in pap-smear samples were investigated from Ahvaz's married women whit PCR method.
Material and Methods: In this cross-sectional study, 276 pap-smear samples from women aged 16-72 years old that refferd to women clinics in 2017 and 2018 years prepared and after DNA extraction, virus identification in samples was performed using specific primers and PCR test.
Results: In this study, 24 samples (%8.7) from married women were contaminated by HSV-2. The highest frequency of infection was observed in the age group of 46-55 years old and in women with normal cytology and mild inflammation.
Conclusions: According to the gathered results it could be concluded that infection prevalence by Herpes virus is more in age group of women with high sexual activity. It could be said that with reference to relatively high infection preference by this virus in active sexually groups, frequency investigation of this virus at 5 years period and programming to increase awareness of general population about disease, its transfer and prevention methods, are necessary and essential to infection decrease in total population at massive level.

Keywords

References

1-Jones C,Cervical cancer: Is Herpes simplex virus 2 a cofactore?” Cli micro rev .1995  8 : 549-556
2-Kukhanova M. K., Korovina A. N, and Kochetkov S. N. Human Herpes Simplex Virus:Life Cycle and Development of Inhibitors.Biochemistry(Moscow).2014.vol:79,no:13,1635-1652
3-Fatahzade M. Schwartz, Robert A . Human Herpes Simpex Virus Infections;Epidemiology, Pathogenesis, Symptomatology, Diagnosis, and Management.. Journal of  the American Academy of Dermatology. 2007.57( 5): 737-763
4-Whitley R J, Kimberlin David W, Roizman Bernard . Herpes simplex virus, IDSA. 1998. 26 : 541-555
5-Whitley R J, Roizman B .Herpes simplex virus infections . The Lancet . 2001.357 :1513-1518.
6-Rathore S, Jamwal A, Gupta V. Herpes simplex virus type 2: Seroprevalence in antenatal women. Indian J Sex Transmited Disease. 2010.31: 11-15
7-Adelson ME, Feola M, Trama J, Tilton RC, Mordechai E. Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and Pyrosequencing. Journal of Clinical Vir.2005.33(1): 25-34
8-Weiss H. Epidemiology of Herpes Simplex Virus type 2 infection in the developing world, Herpes. J of IHME. 2004.11:24-35
9-Pereira V S S, MOIzeis R N C, Fernandes T A A M, Araujo J M G, Meissner R V, Fernandes J V.  Herpes simplex virus type 1 is the main cause of genital herpes in women of Natal, Brazil , European Journal of Obstetrics & Gynecology and Reproductive Biology . 2012.161 :190–193
10-Qutub M, Akhter J. Epidemiology of genital Herpes (HSV-2) among brothel based female sex workers in Bangladesh. European J Epidemiol. 2003. 18: 903-909.
11-Bogaerts J, Ahmed J, Akhter N, Begum N, Rahman M, Nahar S, et al. Sexually transmitted infections among married women in Dhaka, Bangladesh: unexpected high prevalence of Herpes simplex type 2 infection, Sex Transmited Infection. 2001. 77:114-119.
12-Kim I.D,H. Chang S, and Hwang K. J. Herpes simplex virus 2 infection rate and necessity of screening during pregnancy: a clinical and seroepidemiologic study. Yonsei Medical Journal. 2012. 53(2): 401–407.
13-Dolar N, Sedaroglu S  .Seroprevalence of Herpes simplex virus type 1 and 2 in Turkey. journal of European Academy of Dermatology and Venereology . 2006.20 : 1232-1236.
14-Samra Z, Scherf E, Dan M. Herpes simplex virus type 1 is the prevailing cause ofgenital herpes in the Tel Aviv area, Israel. Sex Transm Dis. 200330(10): 794–796.
15-Christopher M, Nikolas R, Catherine S, et al. Multiplex PCR testing detection of higher-than expected rates of cervical Mycoplasma, Ureaplasma, and Trichomonas and viral agent infections in sexually active Australian women. J Clinic Microbial. 2009. 47: 1358-63.
16-Arabzade A.M, Fekri A.R, Shamsadini S. The Prevalence of HSV-2 in Blood Donors in City of Kerman During the Year 2002. Journal of Kerman University of Medical Sciences, 2002; 10(1): 53-59.
18-Pourmand D. Janbakhsh A. Hamzehi K. Dinarvand F. Seroepide Miological Study of Herpes Simplex Virus in Pregnant Women Referring to Health and Care Center in Kermanshah. JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), 2008; 11(4): 462-469.
18-Mofidi M. Saeedi  M. Behnampoor N. Sero-epidemiologic investigation of Herpes Simplex virus type II in Gorgan. 2005. Medical Laboratory Journal. 2014;1 (2) :14-19
19-Ghasemi Dehkordi  P, Momtaz H, Rezaeian A, Yaghobi R,  Molecular study of frequency of Herpes simplex virus type 2in Esfahan and Chaharmahal-va-Bakhtiari provinces in year 1388. Iran J Med Microbiol. 2010; 3 (4) :46-52
20-Aletaha S M, Akya A, Salimi A, Ahmadi K. The Frequency of Herpes Simplex Type 2 Infection in Women with Cervicitis in Kermanshah city in 2011. J Adv Med Biomed Res. 2014; 22 (92) :108-115.
21-Patrick D.M, Dawar M, Darrel A, Krajden M, Rekart M . Antenatal Seroprevalence of Herpes Simplex Virus Type 2(HSV-2) in Canadian Women, Sexually Transmitted Diseases, 2001; 28(7) : 424-428
22-Rathore S, Jamwal A, Gupta V. Herpes simplex virus type 2: Seroprevalence in antenatal women. Indian J Sex Transmited Disease. 2010;31: 11-15
23-Sbouri Ghannad M. Roshanaei Gh. Jafari N. Omidi Z. Habibi H. Seroepidemiology of Herpes simplex virus-2 and affected factors among  females referred to Shahid Beheshti hospital of Hamadan during 2005-2009. Pajouhan Scientific Journal. 2015; 13(3): 23-30.
Jundishapur Scientific Medical Journal
Volume 18, Issue 6 - Serial Number 123
March and April 2020
Pages 631-637

Files

History

  • Receive Date: 09 July 2019
  • Revise Date: 25 January 2020
  • Accept Date: 05 February 2020

Share

How to cite

Statistics