Evaluation of HSP60 and HSP70 Gene Expression Following High-Intensity Anaerobic Training after Ischemia-Reperfusion in Male Rats: Effect of Melatonin

Document Type : Original Article


Department Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran.


Background: The aim of the present study was to evaluate the HSP60 and HSP70 gene expression changes following high intensity anaerobic exercise in male rats after ischemia-reperfusion: the effect of melatonin supplementation.
Methods: For this purpose, 24 male Wistar rats weighing approximately 200-250 g were divided into 4 groups of 6 study groups including control, melatonin, anaerobic training, and anaerobic + melatonin training. The anaerobic exercise protocol was performed for one month, while the melatonin anaerobic training group underwent melatonin gavage during the exercise. At the end of one month, rats were injected with isoprenaline after two days of rest to induce ischemia-reperfusion. After two days the rats were dissected and their left ventricle removed. Then, the expression of HSP60 and HSP70 genes in rats was measured by RNA extraction and Real Time technique.
Results: Results showed that melatonin consumption decreased HSP60 and increased HSP70 apoptotic gene expression after anaerobic exercise (p ≤0.05).
Conclusion: The results of this study showed that intensive anaerobic exercise with melatonin reduced the volume of myocardial apoptosis after ischemia-reperfusion-induced ischemia-reperfusion.


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